{
    "tag": 12963,
    "title": "Cold-water coral microbiomes (Acanthogorgia spp. Desmophyllum dianthus, and Lophelia pertusa) from the Gulf of Mexico and Atlantic Ocean off the southeast coast of the United States: raw sequencing data",
    "pubdate": "20210830",
    "sername": null,
    "series_name": null,
    "issue": null,
    "publish": null,
    "publisher_name": null,
    "onlink": "https:\/\/cmgds.marine.usgs.gov\/catalog\/spcmsc\/PRJNA699458_FGDC_metadata.faq.html",
    "format": null,
    "email": null,
    "descript": "The files provided in this U.S. Geological Survey (USGS) data release (Kellogg and Voelschow, 2021) are the raw DNA sequence files referenced in the associated journal article (Kellogg and Pratte, 2021) entitled, \u201cUnexpected diversity of Endozoicomonas in deep-sea corals.\u201d. This dataset, PRJNA699458_16S-V3V4_raw_data_1.zip, represents the 16S rRNA gene amplicon surveys of 28 samples of deep-sea corals, including Acanthogorgia aspera (n=5), Acanthogorgia spissa (n=4), Desmophyllum dianthus (n=7), and Lophelia pertusa [Desmophyllum pertusum] (n=12), plus a kit extraction control blank. The sequencing targeted the V3-V4 variable region (primers 341F\/806R) and was completed using an Illumina MiSeq sequencing system with version 2 chemistry to obtain paired-end reads.",
    "lang": null,
    "journal": null,
    "pwid": null,
    "originator": [
        {
            "name": "Kellogg, Christina A.",
            "role": "Author"
        },
        {
            "name": "Voelschow, Julie J.",
            "role": "Author"
        }
    ],
    "index_term": [
        {
            "thcode": 2,
            "code": "76",
            "name": "bacteria",
            "scope": "Single celled microorganisms, beneficial or pathogenic, without a nuclear membrane."
        },
        {
            "thcode": 2,
            "code": "92",
            "name": "biodiversity",
            "scope": "The variety in form, genetics, and ecological roles of organisms within a specific geographic area."
        },
        {
            "thcode": 2,
            "code": "311",
            "name": "ecology",
            "scope": "Study of the relations between living plants and animals and their environment."
        },
        {
            "thcode": 2,
            "code": "379",
            "name": "field sampling",
            "scope": "Collecting pieces or specimens or making measurements or observations at determined intervals or areas.  Results may be used as representative of the whole research area or population."
        },
        {
            "thcode": 2,
            "code": "619",
            "name": "laboratory methods",
            "scope": "Techniques to analyze and test samples in a place equipped and designed for the work."
        },
        {
            "thcode": 2,
            "code": "702",
            "name": "marine biology",
            "scope": "Branch of biology concerned with the organisms and habitats of the seas and oceans."
        },
        {
            "thcode": 2,
            "code": "735",
            "name": "microbiology",
            "scope": "Branch of biology dealing with organisms too small to be seen without the use of a microscope."
        },
        {
            "thcode": 15,
            "code": "002",
            "name": "biota",
            "scope": "Flora or fauna in natural environment, for example wildlife, vegetation, biological sciences, ecology, wilderness, sea life, wetlands, habitat, biological resources"
        }
    ],
    "place_term": [],
    "image": [],
    "fan": [
        "13DCM01",
        "2017-381-DD",
        "2018-042-FA",
        "2019-314-FA"
    ]
}