Hope L. Ianiri Nancy G. Prouty 20250218 comma-delimited text data release DOI:10.5066/P13XVXQC Pacific Coastal and Marine Science Center, Santa Cruz, California U.S. Geological Survey https://doi.org/10.5066/P13XVXQC Hope L. Ianiri Nancy G. Prouty Pamela L. Campbell Amy Gartman Katlin B. Adamczyk Jaycee J. Favela 2025 data release DOI:10.5066/P13XVXQC Pacific Coastal and Marine Science Center, Santa Cruz, CA U.S. Geological Survey Suggested Citation: Ianiri, H.L., Prouty, N.G., Campbell, P.L., Gartman, A., Adamczyk, K.B., and Favela, J.J., 2025, Sedimentary organic geochemistry data from Escanaba Trough, off the coast of northern California, collected May-June 2022: U.S. Geological Survey data release, https://doi.org/10.5066/P13XVXQC. https://doi.org/10.5066/P13XVXQC Stable carbon isotope ratios (d13C), stable nitrogen isotope ratios (d15N), and molar percentage data of amino acids were determined on one to five cm subsamples of sediment push cores collected from the Escanaba Trough during May to June 2022. Data were obtained to assess organic carbon storage, reactivity, and cycling within sediments of the Escanaba Trough, a hydrothermal spreading center which contains critical minerals. This work aids our understanding of the geochemistry of marine mineral host sediments and hydrothermal vent processes throughout the global ocean. Additional information about the field activity or activities from which these data were derived is available online at: https:// https://cmgds.marine.usgs.gov/fan_info.php?fan=2022-621-FA Escanaba Trough: Exploring the Seafloor and Oceanic Footprints is an interagency effort involving the National Oceanic and Atmospheric Administration, the U.S. Geological Survey, and the Bureau of Ocean Energy Management. Funding is acknowledged from NOAA Ocean Exploration via its Ocean Exploration Fiscal Year 2019 Funding Opportunity and through the National Oceanographic Partnership Program (NOPP), from the USGS Coastal and Marine Hazards and Resources Enhanced Funding Opportunities, and from the Bureau of Ocean Energy Management through Interagency Agreement M19PG00021 with the United States Geological Survey. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. 20220531 20220611 Ground condition at time data were collected. Complete None planned -127.53618710 -127.44714953 41.08222461 40.69569198 ISO 19115 Topic Category environment oceans geoscientificInformation Data Categories for Marine Planning Nonliving resources Mineral resources Physical/Chemical features Substrate USGS Thesaurus push coring light stable isotope analysis subsampling geochemistry marine geology gas chromatography Marine Realms Information Bank (MRIB) keywords petroleum resources carbon cycle seep and vent ecosystems chemical analysis sediment geochemistry stable isotope analysis None U.S. Geological Survey USGS Coastal and Marine Hazards and Resources Program CMHRP Pacific Coastal and Marine Science Center PCMSC USGS Metadata Identifier USGS:6696d767d34ecb78f609f700 Geographic Names Information System (GNIS) Pacific Ocean NGA GEOnet Names Server (GNS) Escanaba Trough No access constraints USGS-authored or produced data and information are in the public domain from the U.S. Government and are freely redistributable with proper metadata and source attribution. Please recognize and acknowledge the U.S. Geological Survey as the originator(s) of the dataset and in products derived from these data. U.S. Geological Survey, Pacific Coastal and Marine Science Center PCMSC Science Data Coordinator mailing and physical

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Santa Cruz CA 95060 831-427-4747 pcmsc_data@usgs.gov Stephanie Christensen of the University of California, Santa Cruz, assisted with isotope ratio mass spectrometry. Geochemical data are tabulated in comma separated variable (CSV) format. Hope L. Ianiri Pamela L. Campbell Amy Gartman Nancy G. Prouty 2025 Ianiri, H.L., Campbell, P., Gartman, A., and Prouty, N.G., 2025, Characterizing sedimentary organic carbon in a hydrothermal spreading center, the Escanaba Trough: Chemical Geology, https://doi.org/10.1016/j.chemgeo.2025.122679. https://doi.org/10.1016/j.chemgeo.2025.122679 J. L. Morton Robert A. Zierenberg Carol A. Reiss 1994 Morton, J.L., Zierenberg, R.A., Reiss, C.A., 1994. Geologic, hydrothermal, and biologic studies at Escanaba Trough: an introduction, in: Geologic, Hydrothermal, and Biologic Studies at Escanaba Trough, Gorda Ridge, Offshore Northern California. U.S. Geological Survey. David. A. Clague Robert A. Zierenberg Jennifer B. Paduan David W. Caress Brian L. Cousens Brian M. Dreyer Alice S. Davis James McClain Stephanie L. Ross 2022 Clague, D.A., Zierenberg, R.A., Paduan, J.B., Caress, D.W., Cousens, B.L., Dreyer, B.M., Davis, A.S., McClain, J., Ross, S.L., 2022. Emplacement and impacts of lava flows and intrusions on the sediment-buried Escanaba Segment of the Gorda mid-ocean ridge. Journal of Volcanology and Geothermal Research 432, 107701. https://www.sciencedirect.com/science/article/pii/S0377027322002323 Matthew D. McCarthy Ronald Benner Cindy Lee John I. Hedges Marilyn L. Fogel 2004 McCarthy, M.D., Benner, R., Lee, C., Hedges, J.I., Fogel, M.L., 2004. Amino acid carbon isotopic fractionation patterns in oceanic dissolved organic matter: an unaltered photoautotrophic source for dissolved organic nitrogen in the ocean? Mar. Chem. 92, 123-134. https://doi.org/10.1016/j.marchem.2004.06.021 Matthew D. McCarthy Jennifer Lehman Raphael Kudela 2013 McCarthy, M.D., Lehman, J., Kudela, R., 2013. Compound-specific amino acid d 15N patterns in marine algae: Tracer potential for cyanobacterial vs. eukaryotic organic nitrogen sources in the ocean. Geochim. Cosmochim. Acta 103, 104-120. https://doi.org/10.1016/j.gca.2012.10.037 J. A. Silfer M. H. Engel S. A. Macko E. J. Jumeau 1991 Silfer, J.A., Engel, M.H., Macko, S.A., Jumeau, E.J., 1991. Stable carbon isotope analysis of amino acid enantiomers by conventional isotope ratio mass spectrometry and combined gas chromatography/isotope ratio mass spectrometry. Anal. Chem. 63, 370-374. https://doi.org/10.1021/ac00004a014 Yoshinori Takano Yuichiro Kashiyama Nanako O. Ogawa Yoshito Chikaraishi Naohiko Ohkouchi 2010 Takano, Y., Kashiyama, Y., Ogawa, N.O., Chikaraishi, Y., Ohkouchi, N., 2010. Isolation and desalting with cation-exchange chromatography for compound-specific nitrogen isotope analysis of amino acids: application to biogeochemical samples. Rapid Commun. Mass Spectrom. 24, 2317-2323. https://doi.org/10.1002/rcm.4651 Kimio Ueda Stephen L. Morgan Alvin Fox James Gilbart Anders Sonesson Lennart Larsson Goran Odham 1989 Ueda, Kimio., Morgan, S.L., Fox, Alvin., Gilbart, James., Sonesson, Anders., Larsson, Lennart., Odham, Goran., 1989. D-Alanine as a chemical marker for the determination of streptococcal cell wall levels in mammalian tissues by gas chromatography/negative ion chemical ionization mass spectrometry. Anal. Chem. 61, 265-270. https://doi.org/10.1021/ac00178a015 Instrument performance was monitored using both external and internal amino acid standards and directly measured d15N-amino acid and d13C-amino acid values were corrected based on bracketing external standards (McCarthy and others, 2004). The amino acid d13C values were determined from the measured values of the amino acid derivatives following the approach of Silfer and others (1991) with corrections based on an amino acid mixture standard for which isotopic values had been independently determined by offline elemental analyzer analysis. The directly measured amino acid d15N values were also corrected based on bracketing external standards as described in McCarthy and others, 2013. -8888 indicates peak was below the detection limit of 300 mV for carbon and 60 mV for nitrogen. The standard deviation of each isotope and molar percentage value was calculated from triplicate injections. Molar percentage data was checked to ensure the sum of the percentage of all individual amino acids was equivalent to one hundred percent. Dataset is considered complete for the information presented as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. The horizontal accuracy for each sample was obtained from the ROV's Ultra Short Baseline Positioning System (USBL) navigation after calibration to the CASIUS (Calibration of Attitude Sensors In USBL Systems) calibration system. Accuracy for this depth is approximately 10 to 20 m. The vertical accuracy was determined using multiple DVLs (Doppler Velocity Loggers) on the ROV, which are accurate to less than 1 m. SEDIMENT COLLECTION. Samples were collected aboard the R/V Thomas Thompson via push coring using the ROV Jason. Once recovered shipboard, pore waters were extracted via rhizon samplers, cores were subsectioned into one to five cm slices, the exterior sediment which was in contact with the core liner was removed, and remaining sediment was stored frozen in pre-combusted glass jars. 20220611 COMPOUND SPECIFIC ISOTOPE ANALYSIS OF AMINO ACIDS: Amino acids were purified prior to compound specific isotope analysis of amino acids (CSI-AA) according to protocols of the McCarthy lab at the University of California, Santa Cruz (McCarthy and others, 2004). Sediments were subject to acid hydrolysis by adding 6N HCl to each sample, evacuating the headspace with N2 gas, and heating at 110 degrees Celsius for 20 hours. Samples were then mixed, centrifuged at 2200 revolutions per minute for five minutes (min), and supernatant pipetted off to new vial. This process (mixing, centrifuging, and removal of supernatant) was repeated with 1 mL 6N HCl and four 1 milliliter rinses of MilliQ H2O. The supernatant from all HCl and MilliQ H2O rinses were combined and dried under N2 gas at 80 degrees Celsius. Samples were redissolved in 0.1N HCl and a known quantity of Norleucine internal standard was added to each sample. Samples were filtered with pre-combusted glass wool followed by 1.0 micrometer glass fiber filters to remove large particles and then AA were purified using cation-exchange chromatography with Bio-Rad AG50WX8 resin (200-400 mesh) according to Takano and others (2010). Samples were dried under N2 gas at 80 degrees Celsius, redissolved in 0.2N HCl, flushed with N2 gas, and then heated at 110 degrees Celsius for 5 min to re-protonate the amino acids. Trifluoroacetyl isopropyl ester derivatives were prepared after Silfer and others (1991). A final purification step was completed via liquid-liquid extraction using chloroform and a phosphate buffer following Ueda and others (1989). Samples were dried under N2 gas at room temperature, rederivatized, and then stored at 20 degrees Celsius until analysis. Derivatized samples were analyzed for d13C-amino acid and d15N-amino acid isotope values by a Thermo Trace 1310 gas chromatograph coupled to an IsolinkII (Thermo combustion reactor 1000 degrees Celsius)/ConfloIV and Thermo Delta V Plus isotope ratio mass spectrometer. Samples were dried under N2 gas, redissolved in ethyl acetate, and injected in triplicate unless otherwise noted. Amino acids were separated for d15N analyses using a BPX5 column (60 meter x 0.32 millimeter, 1 micrometer film thickness; SGE Analytical Science, Trajan, Austin, TX, USA) and for d13C analyses using a DB-5 column (50 meter x 0.32 millimeter, 0.52 micrometer film thickness; Agilent Technologies, Santa Clara, CA, USA). The injector temperature was 250 degrees Celsius with He column flow rate of 2 mL/min. The GC temperature program for nitrogen isotope analysis was: initial temp = 70 degrees Celsius hold for 1 min; ramp 1=10 degrees Celsius/min to 185 degrees Celsius, hold for 2 min; ramp 2 = 2 degrees Celsius/min to 200 degrees Celsius, hold for 10 min; ramp 3 = 30 degrees Celsius/min to 300 degrees Celsius, hold for 6 min. The GC temperature program for carbon isotope analysis was: initial temp = 75 degrees Celsius hold for 2 min; ramp 1 = 4 degrees Celsius/min to 90 degrees Celsius, hold for 4 min; ramp 2 = 4 degrees Celsius/min to 185 degrees Celsius, hold for 5 min; ramp 3 = 10 degrees Celsius/min to 250 degrees Celsius, hold for 2 min; ramp 4 = 20 degrees Celsius/min to 300 degrees Celsius, hold for 5 min. 20240411 point point 12 0.00000001 0.0000001 Decimal degrees D_WGS_1984 WGS84 6378137.0000 298.2572 EscanabaTrough_CSIAA.csv Table of d13C, d15N, and molar percentage data of amino acids data for subsamples of sediment push cores. producer defined Cruise_ID Lists the alphanumeric cruise identifier for the expedition during which the sample was collected. producer defined TN403 Cruise identification affiliated with Field Activity 2022-621-FA. producer defined Sample_ID Name of sample producer defined The sample names include a dive number (J2, denoting Jason2, followed by a number), push core number (PC, denoting push core, followed by a number) and the sediment depth horizon (X-Y). Latitude Latitude of sample in decimal degrees producer defined 40.69572818 41.08224004 decimal degrees 0.00000001 Longitude Longitude of sample in decimal degrees producer defined -127.5282233 -127.4470967 decimal degrees 0.0000001 Water_depth_m Water depth in meters at which the sample was collected. producer defined 3223 3235 meters 1 Location Location where the sample was collected producer defined Central Hill Text descriptor of sampling site as defined in the literature Morton and others (1994), Clague and others (2022) Edifice Rex East Text descriptor of sampling site as defined in the literature Morton and others (1994), Clague and others (2022) SESCA S3A Text descriptor of sampling site as defined in the literature Morton and others (1994), Clague and others (2022) sediment_depth_top_cm top depth of core subsample. For example, a sediment core slice from 0-1 cm would have a top depth of 0 centimeters and a bottom depth of 1 centimeter. producer defined 0 18 centimeters 1 sediment_depth_bottom_cm bottom depth of core subsample. For example, a sediment core slice from 0-1 cm would have a top depth of 0 centimeters and a bottom depth of 1 centimeter. producer defined 1 20 centimeters 1 Ala_d13C ratio of stable carbon 13/12 isotopes of alanine producer defined -28.9 -19.7 per mil 0.1 Gly_d13C ratio of stable carbon 13/12 isotopes of glycine producer defined -18.9 -10.3 per mil 0.1 Thr_d13C ratio of stable carbon 13/12 isotopes of threonine producer defined -25.2 -10.1 per mil 0.1 Ser_d13C ratio of stable carbon 13/12 isotopes of serine. -9999 indicates not reported due to co-elution with an additional peak. producer defined -9999 -9999 per mil Val_d13C ratio of stable carbon 13/12 isotopes of valine. producer defined -41.6 -24.8 per mil 0.1 Leu_d13C ratio of stable carbon 13/12 isotopes of leucine. producer defined -44.9 -25.7 per mil 0.1 Ile_d13C ratio of stable carbon 13/12 isotopes of isoleucine. producer defined -40.0 -21.0 per mil 0.1 Pro_d13C ratio of stable carbon 13/12 isotopes of proline. producer defined -47.1 -16.2 per mil 0.1 Asp_d13C ratio of stable carbon 13/12 isotopes of aspartic acid and asparagine. producer defined -23.5 -10.0 per mil 0.1 Glu_d13C ratio of stable carbon 13/12 isotopes of glutamic acid and glutamine. producer defined -34.4 -15.7 per mil 0.1 Phe_d13C ratio of stable carbon 13/12 isotopes of phenylalanine. -8888 indicates peak was below detection limit. producer defined -41.2 -24.3 per mil 0.1 Tyr_d13C ratio of stable carbon 13/12 isotopes of tyrosine. -8888 indicates peak was below detection limit. producer defined -41.6 -28.2 per mil 0.1 Lys_d13C ratio of stable carbon 13/12 isotopes of lysine. producer defined -31.4 -13.1 per mil 0.1 Ala_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of alanine measured by triplicate injections. producer defined 0.1 0.5 per mil 0.1 Gly_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of glycine measured by triplicate injections. producer defined 0.1 0.6 per mil 0.1 Thr_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of threonine measured by triplicate injections. producer defined 0.1 1.9 per mil 0.1 Ser_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of serine measured by triplicate injections -9999 indicates not reported due to co-elution with an additional peak. producer defined -9999 -9999 per mil Val_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of valine measured by triplicate injections. producer defined 0.2 3.2 per mil 0.1 Leu_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of leucine measured by triplicate injections. producer defined 0.1 1.3 per mil 0.1 Ile_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of isoleucine measured by triplicate injections. producer defined 0.1 1.4 per mil 0.1 Pro_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of proline measured by triplicate injections. producer defined 0.1 3.8 per mil 0.1 Asp_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of aspartic acid and asparagine measured by triplicate injections. producer defined 0.1 1.7 per mil 0.1 Glu_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of glutamic acid and glutamine measured by triplicate injections. producer defined 0.2 0.9 per mil 0.1 Phe_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of phenylalanine measured by triplicate injections. -8888 indicates peak was below detection limit. producer defined 0.2 2.2 per mil 0.1 Tyr_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of tyrosine measured by triplicate injections. -8888 indicates peak was below detection limit. producer defined 0.3 4.0 per mil 0.1 Lys_SD_d13C standard deviation of the ratio of stable carbon 13/12 isotopes of lysine measured by triplicate injections. producer defined 0.1 4.3 per mil 0.1 Ala_molC molar percentage of alanine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 10.0 18.2 percent 0.1 Gly_molC molar percentage of glycine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 21.2 38.8 percent 0.1 Thr_molC molar percentage of threonine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 3.2 7.4 percent 0.1 Ser_molC molar percentage of serine as calculated from d13C isotope ratio mass spectrometry runs. -9999 indicates not reported due to co-elution with an additional peak. producer defined -9999 -9999 percent Val_molC molar percentage of valine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 3.6 8.2 percent 0.1 Leu_molC molar percentage of leucine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 3.9 9.0 percent 0.1 Ile_molC molar percentage of isoleucine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 1.6 4.9 percent 0.1 Pro_molC molar percentage of proline as calculated from d13C isotope ratio mass spectrometry runs. producer defined 6.2 12.3 percent 0.1 Asp_molC molar percentage of aspartic acid and asparagine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 6.3 15.2 percent 0.1 Glu_molC molar percentage of glutamic acid and glutamine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 4.5 9.2 percent 0.1 Phe_molC molar percentage of phenylalanine as calculated from d13C isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. producer defined 1.1 3.6 percent 0.1 Tyr_molC molar percentage of tyrosine as calculated from d13C isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. producer defined 0.6 1.6 percent 0.1 Lys_molC molar percentage of lysine as calculated from d13C isotope ratio mass spectrometry runs. producer defined 2.9 7.3 percent 0.1 Ala_d15N ratio of stable nitrogen 15/14 isotopes of alanine. producer defined 8.2 23.8 per mil 0.1 Gly_d15N ratio of stable nitrogen 15/14 isotopes of glycine. producer defined -1.0 11.3 per mil 0.1 Thr_d15N ratio of stable nitrogen 15/14 isotopes of threonine. producer defined -2.1 8.2 per mil 0.1 Ser_d15N ratio of stable nitrogen 15/14 isotopes of serine. producer defined -2.8 8.6 per mil 0.1 Val_d15N ratio of stable nitrogen 15/14 isotopes of valine. producer defined 6.2 20.8 per mil 0.1 Leu_d15N ratio of stable nitrogen 15/14 isotopes of leucine. producer defined -2.4 14.4 per mil 0.1 Ile_d15N ratio of stable nitrogen 15/14 isotopes of isoleucine. producer defined -1.3 16.4 per mil 0.1 Pro_d15N ratio of stable nitrogen 15/14 isotopes of proline. producer defined 4.0 16.6 per mil 0.1 Asp_d15N ratio of stable nitrogen 15/14 isotopes of aspartic acid and asparagine. producer defined 5.2 17.7 per mil 0.1 Glu_d15N ratio of stable nitrogen 15/14 isotopes of glutamic acid and glutamine. producer defined 7.0 19.7 per mil 0.1 Phe_d15N ratio of stable nitrogen 15/14 isotopes of phenylalanine. -8888 indicates peak was below detection limit. producer defined -1.7 11.7 per mil 0.1 Tyr_d15N ratio of stable nitrogen 15/14 isotopes of tyrosine. -8888 indicates peak was below detection limit. producer defined -6.1 3.4 per mil 0.1 Lys_d15N ratio of stable nitrogen 15/14 isotopes of lysine. producer defined -4.9 9.1 per mil 0.1 Ala_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of alanine measured by triplicate injections. producer defined 0.3 1.0 per mil 0.1 Gly_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of glycine measured by triplicate injections. producer defined 0.0 0.7 per mil 0.1 Thr_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of threonine measured by triplicate injections. producer defined 0.1 0.4 per mil 0.1 Ser_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of serine measured by triplicate injections. producer defined 0.1 0.6 per mil 0.1 Val_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of valine measured by triplicate injections. producer defined 0.0 0.7 per mil 0.1 Leu_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of leucine measured by triplicate injections. producer defined 0.1 1.8 per mil 0.1 Ile_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of isoleucine measured by triplicate injections. producer defined 0.2 2.0 per mil 0.1 Pro_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of proline measured by triplicate injections. producer defined 0.1 0.5 per mil 0.1 Asp_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of aspartic acid and asparagine measured by triplicate injections. producer defined 0.1 1.1 per mil 0.1 Glu_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of glutamic acid and glutamine measured by triplicate injections. producer defined 0.1 0.5 per mil 0.1 Phe_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of phenylalanine measured by triplicate injections. -8888 indicates peak was below detection limit. producer defined 0.1 1.1 per mil 0.1 Tyr_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of tyrosine measured by triplicate injections. -8888 indicates peak was below detection limit. producer defined 0.4 1.9 per mil 0.1 Lys_SD_d15N standard deviation of the ratio of stable nitrogen 15/14 isotopes of lysine measured by triplicate injections. producer defined 0.1 0.6 per mil 0.1 Ala_molN molar percentage of alanine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 11.4 18.9 percent 0.1 Gly_molN molar percentage of glycine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 19.9 36.0 percent 0.1 Thr_molN molar percentage of threonine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 3.7 6.3 percent 0.1 Ser_molN molar percentage of serine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 2.5 8.9 percent 0.1 Val_molN molar percentage of valine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 2.6 6.5 percent 0.1 Leu_molN molar percentage of leucine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 3.4 8.7 percent 0.1 Ile_molN molar percentage of isoleucine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 1.4 4.2 percent 0.1 Pro_molN molar percentage of proline as calculated from d15N isotope ratio mass spectrometry runs. producer defined 5.8 11.8 percent 0.1 Asp_molN molar percentage of aspartic acid and asparagine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 5.8 13.6 percent 0.1 Glu_molN molar percentage of glutamic acid and glutamine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 6.0 9.5 percent 0.1 Phe_molN molar percentage of phenylalanine as calculated from d15N isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. producer defined 0.6 3.6 percent 0.1 Tyr_molN molar percentage of tyrosine as calculated from d15N isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. producer defined 0.6 1.4 percent 0.1 Lys_molN molar percentage of lysine as calculated from d15N isotope ratio mass spectrometry runs. producer defined 3.2 9.2 percent 0.1 d13C_THAA mole percent weighted average ratio of stable carbon 13/12 isotopes of all amino acids. d13C_THAA = Sum(d13C_AA x molar percent AA/100). producer defined -27.9 -16.3 per mil 0.1 d13C_THAA_SD propagated error of mole percent weighted average ratio of stable carbon 13/12 isotopes of all amino acids. producer defined 0.1 0.5 per mil 0.1 d15N_THAA mole percent weighted average ratio of stable carbon 15/14 isotopes of all amino acids. d15N_THAA = Sum(d15N_AA x molar percent AA). producer defined 1.7 15.0 per mil 0.1 d15N_THAA_SD propagated error of mole percent weighted average ratio of stable nitrogen 15/14 isotopes of all amino acids. producer defined 0.1 0.4 per mil 0.1 OC-AA_percent percentage of total sedimentary organic carbon which is amino acids. producer defined 1.3 8.1 percent 0.1 N-AA_percent percentage of total sedimentary nitrogen which is amino acids. producer defined 4.1 20.4 percent 0.1 The first line of the csv file is a header line. U.S. Geological Survey U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov These data are available in a single csv file (EscanabaTrough_organicgeochemistry_CSIAA.csv). Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. comma-delimited text Microsoft Excel Version 2308 Build 16731.20716 Click-to-Run csv file No compression applied 0.0714 https://www.sciencebase.gov/catalog/file/get/6696d767d34ecb78f609f700 https://www.sciencebase.gov/catalog/item/6696d767d34ecb78f609f700 https://doi.org/10.5066/P13XVXQC Data and metadata can be downloaded using the Network_Resource_Name links. The first link is a direct link to download a zipped file of data and metadata. The second link points to a landing page with metadata and data. The third link points to the landing page for the entire data release. None. These data can be viewed with Microsoft Excel or any text reading software. 20250218 U.S. Geological Survey, Pacific Coastal and Marine Science Center PCMSC Science Data Coordinator mailing and physical

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Santa Cruz CA 95060 831-427-4747 pcmsc_data@usgs.gov Content Standard for Digital Geospatial Metadata FGDC-STD-001-1998