The files provided in this U.S. Geological Survey (USGS) data release (Kellogg and Voelschow, 2021) are the raw DNA sequence files referenced in the associated journal article (Kellogg and Pratte, 2021) entitled, “Unexpected diversity of Endozoicomonas in deep-sea corals.”. This dataset, PRJNA699458_16S-V3V4_raw_data_1.zip, represents the 16S rRNA gene amplicon surveys of 28 samples of deep-sea corals, including Acanthogorgia aspera (n=5), Acanthogorgia spissa (n=4), Desmophyllum dianthus (n=7), and ...

This portion of the data release presents aquatic invertebrate abundance data from samples collected in the Elwha River estuary, Washington, in 2007 and 2013 (no associated USGS Field Activities numbers because data were collected predominantly by biologists from the Lower Elwha Klallam Tribe). Replicate benthic samples were collected at 18 locations throughout the estuary complex using a petite Ponar grab sampler (appx. 2400 mL sample) and sorted through a 500-micron sieve. Samples were fixed in 10 percent ...

This portion of the data release presents fish abundance data from samples collected in the Elwha River estuary, Washington, in 2006, 2007, 2013, and 2014 (no associated USGS Field Activities numbers because data were collected predominantly by biologists from the Lower Elwha Klallam Tribe). We used the Puget Sound beach seining protocol (Simenstad and others, 1991) to sample fish populations in the Elwha River estuary complex. The beach seine was 38 m long x 2 m deep, with a 2 m x 2 m bag in the center of ...

This portion of the data release presents fish diet data from Chinook and coho salmon collected in the Elwha River estuary, Washington, in 2006, 2007, 2013, and 2014 (there are no associated USGS Field Activities numbers because data were collected predominantly by biologists from the Lower Elwha Klallam Tribe). Fish were collected using a beach seine at six locations throughout the estuary. Fish were transferred to buckets containing aerated ambient water and kept cool until handling. We anesthetized fish ...

This portion of the data release presents terrestrial invertebrate abundance data from samples collected in emergent and shrub vegetation along the edge of the Elwha River estuary, Washington, in 2007 and 2013 (no associated USGS Field Activities numbers because data were collected predominantly by biologists from the Lower Elwha Klallam Tribe). We deployed terrestrial insect fallout traps at ten locations in the east estuary, five replicates each in shrub and emergent (littoral) vegetation habitats. Clear, ...

Due to the recognized proliferation and spread of antibiotic resistance genes by anthropogenic use of antibiotics for human, agriculture and aquaculture purposes, antibiotic resistance genes have been defined as an emerging contaminant (Laxminarayan and others, 2013; Rodriguez-Rojas and others, 2013; Niu and others, 2016). The presence and spread of these genes in non-clinical and non-agricultural environments has created the need for background investigations to enhance our understanding of the magnitude ...

The prevalence of antibiotic resistance genes in microbial communities from sewage wastewater streams and from offshore marine sediments in the vicinity of sewage wastewater outfalls in Southeast Florida was investigated from June 2018 to March 2019. Sediment and wastewater samples were analyzed for 15 different antibiotic resistant gene targets via polymerase chain reaction (PCR) presence/absence assays in Southeast Florida coral reef environments. Data collected from five sites (Broward North Wastewater ...

This data set includes: (1) allele sizes of 11 previously published microsatellites (Kenkel and others, 2013; Shearer and Coffroth, 2004) for 39 individuals of mustard hill coral, Porites astreoides (mustard hill coralP. astreoides) collected in the Spring of 2017 from four locations in the Florida Keys Reef Tract (FKRT): Fowey Rocks, Crocker Reef, Sombrero Reef and Pulaski Shoal and (2) the deoxyribonucleic acid (DNA) concentration of the extracted DNA prior to polymerase chain reaction (PCR) reactions for ...

Due to the recognized proliferation and spread of antibiotic resistance genes by anthropogenic use of antibiotics for human, agriculture and aquaculture purposes, antibiotic resistance genes have been defined as an emerging contaminant (Laxminarayan and others, 2013; Rodriguez-Rojas and others, 2013; Niu and others, 2016). The presence and spread of these genes in non-clinical and non-agricultural environments has created the need for background investigations to enhance our understanding of the magnitude ...