Microbial Processes Contributing to the Clogging of Aquifer Storage and Recovery (ASR) Wells in South Florida

Metadata also available as - [Outline] - [Parseable text] - [XML]

Frequently anticipated questions:

What does this data set describe?

Title:
Microbial Processes Contributing to the Clogging of Aquifer Storage and Recovery (ASR) Wells in South Florida
Abstract:
This metadata record describes data collected from laboratory experiments designed to characterize the microbial processes that contribute to clogging (i.e., bioclogging) of wells used for recharge (i.e., injection) of fresh surface water into specific aquifer zones (Upper Floridan Aquifer [UFA] and Avon Park Permeable Zone [APPZ]) as part of water storage technology of aquifer storage and recovery (ASR). Solid rock core samples were collected from three wells (ASRC38S, ASRL63S and ASRC59; abbreviated to C38S, L63S, and C59 henceforth) near Lake Okeechobee, Florida, that have been designated as injection well sites for ASR wells as part of the Comprehensive Everglades Restoration Plan, on July 22, 2022 (C38S/UFA), May 22, 2023 (C38S/APPZ), July 7, 2023 (L63S/UFA), July 13, 2023 (L63S/APPZ) and November 15, 2023 (C59/UFA and C59/APPZ). The cores were crushed and sieved to a specific size, and this sized material was used to pack four columns: two columns for each well and aquifer storage zone (e.g., C38S/UFA and C38S/APPZ) (ATSM International, 2020; Rinck-Pfeiffer and others, 2000). Within each set of paired columns, one column was allowed to be colonized by native groundwater microbial communities (i.e., C38S/UFA Biofilm Positive column) by being connected to a continuously flowing source of native groundwater, either the UFA or APPZ, depending on the source of the packed core materials, for between two and four months. Prior to starting each experiment, pressure sensors were inserted into each column (Rinck-Pfeiffer and others, 2000). Each experiment was initiated by respectively pumping native groundwater from the UFA or APPZ into the UFA/Biofilm Positive and Negative and APPZ/Biofilm Positive and Negative columns for one week to geochemically condition the columns before starting the recharge phases of the experiments. Water collected from the Kissimmee River confluence with Lake Okeechobee was used as the recharge source water and was pumped through both the UFA Biofilm Positive and Negative and APPZ Biofilm Positive and Negative columns. During each experiment, pressure data in kilopascals (kPa) were recorded at specific locations along the vertical axis of all columns (Rinck-Pfeiffer and others, 2000). Additionally, pump rates into the columns were closely maintained at 5.00 milliliters per minute (mL/min). These data were used to calculate the hydraulic conductivity (meters/day) for all experiments (Sanchez-Vila and others, 2006). In addition to column pressure and flow rate data being collected, samples were also collected from native groundwater sources, Kissimmee River and from the column inputs, discharges and porewater after a static storage period for geochemistry, nutrients, dissolved gases, and organic carbon substrates to characterize microbial community preferential growth rates on selected organic carbon substrates.
Supplemental_Information:
Following U.S. Geological Survey St. Petersburg Coastal and Marine Science Center (USGS SPCMSC) data management protocols, these data were assigned USGS field activity number (FAN), 2024-314-FA. Additional survey and data details are available on the Coastal and Marine Geoscience Data System (CMGDS) at https://cmgds.marine.usgs.gov/fan_info.php?fan=2024-314-FA.
  1. How might this data set be cited?
    Lisle, John T., and Williams, Breanna N., 20250311, Microbial Processes Contributing to the Clogging of Aquifer Storage and Recovery (ASR) Wells in South Florida:.

    This is part of the following larger work.

    Lisle, John T., and Williams, Breanna N., 20250311, Microbial Processes Contributing to the Clogging of Aquifer Storage and Recovery (ASR) Wells in South Florida: U.S. Geological Survey data release doi:10.5066/P1GN7EN6, U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center, St. Petersburg, Florida.

    Online Links:

  2. What geographic area does the data set cover?
    West_Bounding_Coordinate: -80.875267
    East_Bounding_Coordinate: -80.604618
    North_Bounding_Coordinate: 27.193770
    South_Bounding_Coordinate: 26.990679
  3. What does it look like?
  4. Does the data set describe conditions during a particular time period?
    Beginning_Date: 22-Jul-2022
    Ending_Date: 31-Jan-2025
    Currentness_Reference:
    ground condition
  5. What is the general form of this data set?
    Geospatial_Data_Presentation_Form: tabular digital data
  6. How does the data set represent geographic features?
    1. How are geographic features stored in the data set?
    2. What coordinate system is used to represent geographic features?
  7. How does the data set describe geographic features?
    Entity_and_Attribute_Overview:
    Bioclogging_Geochemistry.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of the geochemical analyses on samples collected from the native UFA, APPZ, and Kissimmee River samples and column inputs, discharges, and post-storage porewaters. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
    Entity_and_Attribute_Overview:
    Bioclogging_Nutrients.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of the nutrient analyses on samples collected from the native UFA, APPZ, and Kissimmee River samples and column inputs, discharges, and post-storage porewaters. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
    Entity_and_Attribute_Overview:
    Bioclogging_Dissolved_Gases.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of the dissolved gases in samples collected from the native UFA, APPZ, and Kissimmee River samples and column inputs, discharges, and post-storage porewaters. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
    Entity_and_Attribute_Overview:
    Bioclogging_BDOC.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of the dissolved organic carbon (DOC) from samples collected during biodegradable organic carbon (BDOC) experiments. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
    Entity_and_Attribute_Overview:
    Bioclogging_Hydraulic_Conductivity.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of the hydraulic conductivity within the respective columns for each experiment. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
    Entity_and_Attribute_Overview:
    Bioclogging_Substrate_Growth.zip: Zipped file containing the comma-separated values (.csv) and Microsoft Excel (.xlsx) data files of absorbance and substrate diversity data from the EcoPlate™ analyses at specific incubation time points. Detailed attribute descriptions for these files are included a data dictionary, Bioclogging_Data_Dictionary.docx. These metadata are not complete without this file.
    Entity_and_Attribute_Detail_Citation:
    The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.

Who produced the data set?

  1. Who are the originators of the data set? (may include formal authors, digital compilers, and editors)
    • John T. Lisle
    • Breanna N. Williams
  2. Who also contributed to the data set?
    Data collection was made possible by collaborative efforts between the U.S. Geological Survey St. Petersburg Coastal and Marine Science Center and the South Florida Water Management District (SFWMD). Sample analyses were completed by Eurofins Environment Testing Tampa, University of Tampa's Analytical & Marine Chemistry Laboratory, Stratum Reservoir/Isotech Laboratories, Inc., and the Florida International University's Kominoski Laboratory for Ecosystem Ecology.
  3. To whom should users address questions about the data?
    John T. Lisle
    U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center
    Research Microbiologist
    600 4th Street South
    St. Petersburg, Florida
    United States

    727-502-8140 (voice)
    jlisle@usgs.gov

Why was the data set created?

Data were collected to determine the rates at which specific microbial processes contribute to the clogging of recharge (i.e., injection) wells that receive surface water as part of an ASR facility.

How was the data set created?

  1. From what previous works were the data drawn?
  2. How were the data generated, processed, and modified?
    Date: 15-Nov-2023 (process 1 of 7)
    Solid rock cores (2.5 inches in diameter) were collected near Lake Okeechobee, Florida, on July 22, 2022 (C38S/UFA), May 22, 2023 (C38S/APPZ), July 7, 2023 (L63S/UFA), July 13, 2023 (L63S/APPZ) and November 15, 2023 (C59/UFA and C59/APPZ) by private drilling companies contracted by SFWMD (3301 Gun Club Road, West Palm Beach, Florida 33406). The cores were drilled and recovered from three wells from specific aquifer zones, which had been identified as recharge zones for ASR, using the wireline drilling system. These wells are approximately located at: C38S (27.147958, -80.873387), L63S (27.157525, -80.677441), and C59 (27.19377, -80.760747) (see https://apps.sfwmd.gov/dbhydroInsights/ for additional information on these ASR well sites).
    Date: 23-Nov-2023 (process 2 of 7)
    Lengths of core from specific depths were selected and packaged by a SFWMD contracted engineering company (Stantec), then shipped to a vendor (Core Laboratories) for crushing and sizing each core sample. Person who carried out this activity:
    Stantec
    2056 Vista Parkway
    West Palm Beach, Florida
    United States

    561-686-7707 (voice)
    askstantec@stantec.com
    Date: 2023 (process 3 of 7)
    The selected lengths of core were shipped to a vendor (Core Laboratories) that specializes in the crushing and sizing of rock cores to specific sizes. All cores were dried and then crushed to a #20 sieve size (850 micrometers [μm]) (ATSM International, 2020). This material was used to pack a series of columns, to be used in the bioclogging experiments, as described in the following sections. Person who carried out this activity:
    Core Laboratories
    6316 Windfern Road
    Houston, Texas
    United States

    713-328-2673 (voice)
    Date: 31-Jan-2025 (process 4 of 7)
    From 2022 to 2025, after the cores were crushed and sieved to a specific size, this sized material was used to pack four columns: two columns for each well and aquifer storage zone (e.g., C38S/UFA and C38S/APPZ) (Rinck-Pfeiffer and others, 2000). Within each set of paired columns, one column was allowed to be colonized by native groundwater microbial communities (i.e., C38S/UFA Biofilm Positive column) by being connected to a continuously flowing source of native groundwater, either the UFA (27.154792, -80.874273) or APPZ (26.990679, -80.604618), depending on the source of the packed core materials, for between two and four months. Prior to starting each experiment, pressure sensors were inserted into each column. Each experiment was initiated by respectively pumping native groundwater from the UFA or APPZ into the UFA/Biofilm Positive and Negative and APPZ/Biofilm Positive and Negative columns at a rate of 5.0 mL/min for one week to geochemically condition the columns before starting the recharge phases of the experiments (referred as the "Conditioning Phase"). Water collected from the Kissimmee River confluence with Lake Okeechobee (27.154451, -80.875267) was used as the recharge source water and was pumped through both the UFA Biofilm Positive and Negative and APPZ Biofilm Positive and Negative columns. The "Conditioning Phase" was immediately followed by the "1st Recharge Phase", during which collected surface water from the Kissimmee River was pumped at the same rate through all columns for 4-6 weeks. At the end of the "1st Recharge Phase", the pumping of Kissimmee River water was terminated, and all columns were valved off to contain the water in all columns in a static phase for 4-5 weeks. This experimental phase was called the "Storage Phase". Following the "Storage Phase", the "2nd Recharge Phase" was initiated by pumping Kissimmee River water through all columns at the same rate for 2-3 weeks. Person who carried out this activity:
    John T. Lisle
    U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center
    Research Microbiologist
    600 4th Street South
    St. Petersburg, Florida
    United States

    727-502-8140 (voice)
    jlisle@usgs.gov
    Date: 31-Jan-2025 (process 5 of 7)
    During the "1st Recharge Phase" to the end of the "2nd Recharge Phase", pressure within each column was collected using digital pressure sensors. Pump rates of water being pumped into each column were recorded daily. Pressures within each column were collected using pressure transducers (CirrusSense™ TDWLB Wireless Pressure Transducer), 4 per column, that reported the respective pressures in kPa (Rinck-Pfeiffer and others, 2000). The hydraulic conductivities listed in this data release are the calculated values using the pump rates and column pressures (Sanchez-Vila and others, 2006). Person who carried out this activity:
    John T. Lisle
    U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center
    Research Microbiologist
    600 4th Street South
    St. Petersburg, Florida
    United States

    727-502-8140 (voice)
    jlisle@usgs.gov
    Data sources produced in this process:
    • Bioclogging_Hydraulic_Conductivity.csv
    • Bioclogging_Hydraulic_Conductivity.xlsx
    Date: 31-Jan-2025 (process 6 of 7)
    A set of experiments to determine the rates at which native microbial communities from the UFA and APPZ utilize the carbon substrates (biodegradable organic carbon; BDOC) in the Kissimmee River was conducted and did not require access to water being pumped into or stored in the "Biofilm Positive" or "Biofilm Negative" columns. Samples were analyzed for dissolved organic carbon by the Florida International University's Kominoski Laboratory for Ecosystem Ecology (Anderson and others 2024). Person who carried out this activity:
    Florida International University's Kominoski Laboratory for Ecosystem Ecology
    11200 SW 8th Street, OE 148
    Miami, Florida
    United States

    305-348-7117 (voice)
    jkominos@fiu.edu
    Data sources produced in this process:
    • Bioclogging_BDOC.csv
    • Bioclogging_BDOC.xlsx
    Date: 31-Jan-2025 (process 7 of 7)
    Water samples were collected during the "Conditioning Phase" and "1st Recharge Phase", after the completion of the "Storage Phase" (i.e., "Post Storage"). Native UFA, APPZ, and Kissimmee River water samples were used for the analysis of geochemistry, nutrients, dissolved gases, and carbon substrate growth rates from Biolog® EcoPlates™ that were scanned at 590 nanometers (nm) wavelength. The carbon substrate growth rate data analyses were completed by the USGS SPCMSC using the Biolog® MicroStation™ system with the Biolog® MicroLog 3 (release 4.20) software (Weber and Legge, 2010). Geochemical analyses were completed by the Eurofins Environment Testing Tampa (6712 Benjamin Road, Office #100, Tampa, Florida 33634). Nutrient analyses were completed by the University of Tampa's Analytical & Marine Chemistry Laboratory (401 West Kennedy Boulevard, Tampa, Florida 33606) (Lisle, 2020). Dissolved gases analyses were completed by Stratum Reservoir/Isotech Laboratories, Inc. (1308 Parkland Court, Champaign, Illinois 61821) (Vigneron, and others, 2017). Data sources produced in this process:
    • Bioclogging_Substrate_Growth.csv
    • Bioclogging_Substrate_Growth.xlsx
    • Bioclogging_Nutrients.csv
    • Bioclogging_Nutrients.xlsx
    • Bioclogging_Geochemistry.csv
    • Bioclogging_Geochemistry.xlsx
    • Bioclogging_Dissolved_Gases.csv
    • Bioclogging_Dissolved_Gases.xlsx
  3. What similar or related data should the user be aware of?
    International, ATSM, 20200122, ASTM C136/C136M-19: Standard test method for sieve analysis of fine and coarse aggregates: ASTM International, West Conshohocken, PA.

    Online Links:

    Rinck-Pfeiffer, Stéphanie, Ragusa, Santo, Sztajnbok, Pascale, and Vandevelde, Thierry, 20000221, Interrelationships between biological, chemical, and physical processes as an analog to clogging in aquifer storage and recovery (ASR) wells: Water Research Volume 34, Issue 7, Elsevier, Online.

    Online Links:

    Other_Citation_Details: Pages 2110-2118
    Sanchez-Vila, Xavier, Guadagnini, Alberto, and Carrera, Jesus, 20060923, Representative hydraulic conductivities in saturated groundwater flow: Reviews of Geophysics Volume 44, Issue 3, Wiley, Online.

    Online Links:

    Other_Citation_Details: 46 pages
    Anderson, Kenneth J., Kominoski, John S., Osburn, Christopher L., and Smith, Matthew A., 20240629, Shifting sources and fates of carbon with increasing hydrologic presses and pulses in coastal wetlands: JGR Biogeosciences Volume 129, Issue 7, Wiley, Online.

    Online Links:

    Other_Citation_Details: 17 pages
    Weber, Kela P., and Legge, Raymond L., 2010, Community-level physiological profiling: Bioremediation Volume 599, Humana Press, Online.

    Online Links:

    Other_Citation_Details: Pages 263-281
    Lisle, John T., 20200728, Nutrient removal and uptake by native planktonic and biofilm bacterial communities in an anaerobic aquifer: Frontiers in Microbiology Volume 11, Article 1765, Frontiers, Online.

    Online Links:

    Other_Citation_Details: 13 pages
    Vigneron, Adrien, Bishop, Andrew, Alsop, Eric B., Hull, Kellie, Rhodes, Ileana, Hendricks, Robert, Head, Ian M., and Tsesmetzis, Nicolas, 20170404, Microbial and isotopic evidence for methane cycling in hydrocarbon-containing groundwater from the Pennsylvania region: Frontiers Microbiology Volume 8, Article 593, Frontiers, Online.

    Online Links:

    Other_Citation_Details: 12 pages

How reliable are the data; what problems remain in the data set?

  1. How well have the observations been checked?
    No formal attribute accuracy tests were conducted.
  2. How accurate are the geographic locations?
  3. How accurate are the heights or depths?
  4. Where are the gaps in the data? What is missing?
    Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
  5. How consistent are the relationships among the observations, including topology?
    No formal logical accuracy tests were conducted.

How can someone get a copy of the data set?

Are there legal restrictions on access or use of the data?
Access_Constraints No access constraints. Please see 'Distribution Information' for details.
Use_Constraints These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints.
  1. Who distributes the data set? (Distributor 1 of 1)
    U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
    Attn: USGS SPCMSC Data Management
    600 4th Street South
    Saint Petersburg, Florida
    United States

    727-502-8000 (voice)
    gs-g-spcmsc_data_inquiries@usgs.gov
  2. What's the catalog number I need to order this data set? Bioclogging_Hydraulic_Conductivity.csv, Bioclogging_Hydraulic_Conductivity.xlsx, Bioclogging_BDOC.csv, Bioclogging_BDOC.xlsx, Bioclogging_Substrate_Growth.csv, Bioclogging_Substrate_Growth.xlsx, Bioclogging_Nutrients.csv, Bioclogging_Nutrients.xlsx, Bioclogging_Geochemistry.csv, Bioclogging_Geochemistry.xlsx, Bioclogging_Dissolved_Gases.csv, Bioclogging_Dissolved_Gases.xlsx, and Bioclogging_Data_Dictionary.docx.
  3. What legal disclaimers am I supposed to read?
    Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
  4. How can I download or order the data?

Who wrote the metadata?

Dates:
Last modified: 11-Mar-2025
Metadata author:
U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
Attn: USGS SPCMSC Data Management
600 4th Street South
Saint Petersburg, Florida
United States

727-502-8000 (voice)
gs-g-spcmsc_data_inquiries@usgs.gov
Metadata standard:
Content Standard for Digital Geospatial Metadata (FGDC-STD-001-1998)
This page is <https://cmgds.marine.usgs.gov/catalog/spcmsc/Bioclogging_metadata.faq.html>
Generated by mp version 2.9.51 on Tue Mar 11 10:54:35 2025