Metadata: Identification_Information: Citation: Citation_Information: Originator: Dale Griffin Originator: Kenneth Banks Originator: Kurtis Gregg Originator: Sarah Shedler Originator: Brian Walker Publication_Date: 20200218 Title: Southeast Florida and Florida Keys Antibiotic Resistance Study Geospatial_Data_Presentation_Form: Tabular Digital Data Series_Information: Series_Name: U.S. Geological Survey Data Release Issue_Identification: doi:10.5066/P98KQWDN Publication_Information: Publication_Place: Reston, VA Publisher: U.S. Geological Survey Online_Linkage: https://doi.org/10.5066/P98KQWDN Description: Abstract: The prevalence of antibiotic resistance genes in microbial communities from sewage wastewater streams and from offshore marine sediments in the vicinity of sewage wastewater outfalls in Southeast Florida was investigated from June 2018 to March 2019. Sediment and wastewater samples were analyzed for 15 different antibiotic resistant gene targets via polymerase chain reaction (PCR) presence/absence assays in Southeast Florida coral reef environments. Data collected from five sites (Broward North Wastewater Treatment Plant (WWTP), Broward North WWTP Outfall, Haµlover (Miami-Dade North) Outfall, Hollywood Outfall, Hollywood WWTP, and Miami-Dade North WWTP) illustrated widespread prevalence of antibiotic resistance genes in these microbial communities with the highest concentrations occurring in the sewage wastewater stream and in close proximity to the outfall pipe and outfall. Data indicated seasonal (wet versus dry season) trends and potential public and ecosystem health risks. Additionally, a reef in the Florida Keys was evaluated during the wet season using the same tools and approach prior to and after diseased corals were treated with amoxicillin. Resistance to amoxicillin was only observed in the post-treatment sample set. Purpose: The objective of this project was to determine the prevalence of 15 antibiotic resistant genes (tetB, tetG, tetL, tetM, tetO, tetQ, tetW, ampC, vanA, ermB, mecA, blaSHV, blaPSE, floR, and aadA2) in bacterial popµlations in impacted (close to and alongside outfall pipes and within the wastewater stream) and control (along transects extending away from the outfalls) sediment samples around the Hollywood, FL wastewater treatment plantÕs ocean outfall. A total of 38 wet season and 42 dry season samples were collected by the Florida Department of Environmental Protection, Broward County and National Oceanic and Atmospheric Administration (NOAA) research teams and shipped to and analyzed by scientists at the U.S. Geological Survey (USGS) St. Petersburg Environmental and Public Health Microbiology Laboratory. Time_Period_of_Content: Time_Period_Information: Range_of_Dates/Times: Beginning_Date: 20180611 Ending_Date: 20190311 Currentness_Reference: ground condition Status: Progress: Complete Maintenance_and_Update_Frequency: Not planned Spatial_Domain: Bounding_Coordinates: West_Bounding_Coordinate: -79.9 East_Bounding_Coordinate: -82.5 North_Bounding_Coordinate: 26.5 South_Bounding_Coordinate: 24.3 Keywords: Theme: Theme_Keyword_Thesaurus: USGS Metadata Identifier Theme_Keyword: USGS:e0a6aa24-88d6-46c6-a4e8-df501aacffc0 Theme: Theme_Keyword_Thesaurus: ISO 19115 Topic Category Theme_Keyword: environment Theme_Keyword: biota Theme_Keyword: health Theme: Theme_Keyword_Thesaurus: USGS Thesaurus Theme_Keyword: field monitoring stations Theme_Keyword: contamination and pollution Theme_Keyword: microbiology Theme_Keyword: genetic diversity Theme: Theme_Keyword_Thesaurus: None Theme_Keyword: sewage outfalls Theme_Keyword: environmental health (human and ecosystem) Theme_Keyword: coral reef sediment Theme_Keyword: antibiotic resistance Place: Place_Keyword_Thesaurus: Geographic Names Information System Place_Keyword: Florida Keys Place_Keyword: Miami-Dade County Place_Keyword: Broward County Stratum: Stratum_Keyword_Thesaurus: Oregon GEO Stratum Keywords Stratum_Keyword: Sediment Temporal: Temporal_Keyword_Thesaurus: Oregon GEO temporal Keywords Temporal_Keyword: Modern Access_Constraints: None Use_Constraints: Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey requests to be acknowledged as originator of these data in future products or derivative research. Point_of_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Dale W. Griffin Contact_Organization: U.S. Geological Survey, Southeast Region Contact_Position: Environmental and Public Health Microbiologist Contact_Address: Address_Type: mailing address Address: 600 4Th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: USA Contact_Voice_Telephone: 727-502-8075 Contact_Facsimile_Telephone: 727-502-8182 Contact_Electronic_Mail_Address: dgriffin@usgs.gov Native_Data_Set_Environment: Environment as of Metadata Creation: Microsoft Windows 7 Version 6.1 (Build 7601) Service Pack 1; Esri ArcGIS 10.3.1 (Build 4959) Service Pack N/A (Build N/A) Cross_Reference: Citation_Information: Originator: Dale W. Griffin Originator: Kenneth Banks Originator: Kurtis Gregg Originator: Sarah Shedler Originator: Brian K. Walker Publication_Date: 20200311 Title: Antibiotic Resistance in Marine Microbial Communities Proximal to a Florida Sewage Outfall System Series_Information: Series_Name: Antibiotics Issue_Identification: 9(3):118 Online_Linkage: https://doi.org/10.3390/antibiotics9030118 Cross_Reference: Citation_Information: Originator: Shawn C. Fisher Originator: Timothy J. Reilly Originator: Daniel K. Jones Originator: William M. Benzel Originator: Dale W. Griffin Originator: Keith A. Loftin Originator: Luke R. Iwanowicz Originator: Jonathan A. Cohl Publication_Date: 20151217 Title: Standard operating procedure for collection of soil and sediment samples for the Sediment-bound Contaminant Resiliency and Response (SCoRR) strategy pilot study Series_Information: Series_Name: U.S. Geological Survey Open-File Report Issue_Identification: 2015-1188b Online_Linkage: https://doi.org/10.3133/ofr20151188B Data_Quality_Information: Attribute_Accuracy: Attribute_Accuracy_Report: No formal attribute accuracy tests were conducted. Logical_Consistency_Report: No formal logical accuracy tests were conducted. Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefµlly for additional details. Positional_Accuracy: Horizontal_Positional_Accuracy: Horizontal_Positional_Accuracy_Report: A formal accuracy assessment of the horizontal positional information in the dataset has not been conducted. Vertical_Positional_Accuracy: Vertical_Positional_Accuracy_Report: A formal accuracy assessment of the vertical positional information in the dataset has either not been conducted or is not applicable. Lineage: Process_Step: Process_Description: Sediment, coral mucus and water samples acquired from Southeast Florida between 2018 and 2019 were analyzed for 15 different antibiotic resistant gene targets via PCR presence/absence assays. The SCoRR standard operating procedure (SOP) (Fisher and others, 2015) details a related study using similar collection methods to those utilized for this project with the exception of the coral mucus samples, which were collected by aspiration, using a sterile 60 cubic centimeter plastic syringe. The SOP provides step-by-step instructions for site preparation, sample processing, quality assurance, and shipping of soil and surficial bed sediment. Process_Date: 20180601 Process_Step: Process_Description: DNA extraction was performed by first calibrating the Mettler scale with a 50-gram (g) certified weight and then documenting that value on the Mettler QA/QC sheet. Using sterile-techniques, approximately 0.25 g of sediment or 200 microliters (µl) of water from each sample was weighted out or pipetted and transferred to a MoBio PowerSoil bead beating tube. Sample weight or volume was logged in the project electronic laboratory notebook (ELN). The MoBio PowerSoil kit protocol was used to elute DNA to a volume of 100 µl using Qiagens AE buffer recipe (10 millimolar (mM) Tris-HCL, 0.5 mM EDTA, pH 9.0, filter sterilized and autoclaved). Eluted DNA was stored at -70 degrees Celsius (¡C) until use. Process_Date: 20180706 Process_Step: Process_Description: The following recipes and plate(s) layouts were used to set up quantitative polymerase chain reaction (qPCR) reactions. All reactions were run in duplicate and positives were those reactions where amplification was noted in both reactions that reached a normalized reporter signal (Rn) value of 0.1 (with an exponential trend). All duplicate positives were screened with digital PCR. Master Mix recipes (volumes per reaction) are as follows: Single probe reactions Ð 20 µl reactions - row 1 Internal Positive Control (IPC): 2x TaqMan Fast Universal Master Mix (10 µl); Template (2 µl); Primer/probe (10 micrometers (µm)/5 µm working stock) (1 µl each); 10x Exo Internal Positive Control (2 µl); 50x EXo IPC DNA (0.4 µl); PCR grade water (4.6 µl). IPC control reactions - row 8: 2x TaqMan Fast Universal Master Mix (10 µl); Template (2 µl); 10x Exo Internal Positive Control (2 µl); 50x EXo IPC DNA (0.4 µl); PCR grade water (5.6 µl). Mµltiplex (two primer and probe sets) Ð rows 2 through 7: 2x TaqMan Fast Universal Master Mix (10 µl); Template (2 µl); primer/probe (10 µm/5 µm working stock) (1 µl each); PCR grade water (6.0 µl). PCR amplification profile Master details are as follows: Pre PCR read Ð 60 ¡C for 0.5 minutes (min); Holding stage Ð 50 ¡C for 2 min then 95 ¡C for 5 min; Cycling stage Ð 45 cycles of 95 ¡C for 0.25 min then 60 ¡C for 1 min; Post PCR read Ð 60 ¡C for 0.5 min. Ninety-six well PCR plate 1 layout - row 1 = tetB/IPC, row 2 = tetL/M, row 3 = tetO/W, row 4 = ampC/vanA, row 5 = ermB/mecA, row 6 = blaSHV/blaPSE, row 7 = floR/aadA2, row 8 = controls (PCR-grade water for template Ð columns 9 and 10) and IPC negative control (2 µl of IPC block Ð columns 11 and 12). Ninety-six well PCR plate 2 layout - row 1-7 = tetG/tetQ for all the samples and the same layout of controls in row 8 as described for plate 1. Two dyes, FAM and VIC, were used to monitor amplification in the real-time PCR reaction assays. FAM-labeled probes are tetB, tetL, tetO, ampC, ermB, blaSHV, floR, and tetG. VIC-labeled probes are the IPC, TetM, TetW, vanA, mecA, blaPSE, aadA2, and tetQ. Process_Date: 20180709 Process_Step: Process_Description: Added keywords section with USGS persistent identifier as theme keyword. Process_Date: 20201013 Process_Contact: Contact_Information: Contact_Organization_Primary: Contact_Organization: U.S. Geological Survey Contact_Person: VeeAnn A. Cross Contact_Position: Marine Geologist Contact_Address: Address_Type: Mailing and Physical Address: 384 Woods Hole Road City: Woods Hole State_or_Province: MA Postal_Code: 02543-1598 Contact_Voice_Telephone: 508-548-8700 x2251 Contact_Facsimile_Telephone: 508-457-2310 Contact_Electronic_Mail_Address: vatnipp@usgs.gov Entity_and_Attribute_Information: Detailed_Description: Entity_Type: Entity_Type_Label: WetSeason_PCR_data_sheet.csv Entity_Type_Definition: Table containing attribute information associated with the dataset. Presence/absence qPCR data are provided in comma separated values format. Entity_Type_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: Tube # Attribute_Definition: Laboratory-assigned tube number. Keys 1-24 contain samples collected from the Florida Keys and Broward 1-38 includes those acquired from Broward County. Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: Sample identifier Attribute: Attribute_Label: Sample type Attribute_Definition: Type of sample (sediment, water, or coral mucus) collected at each sampling location. Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: Samples obtained from Coffins Patch, a shallow coral reef located within the Florida Keys National Marine Sanctuary, were collected prior to and after diseased corals were treated with amoxicillin. Theses coral specimens had Ò -pretreatmentÓ or Ò- post treatmentÓ appended to their sample type. For example, sediment - post treatment, mucus - pretreatment, etc. Attribute: Attribute_Label: Sample site Attribute_Definition: Sample site location Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: A total of 80 samples were collected from mµltiple sites by FDEP, Broward County and NOAA staff. Sample sites included: Broward North WWTP, Broward North WWTP Outfall, Haµlover (Miami-Dade North) Outfall, Hollywood Outfall, Hollywood WWTP, and Miami-Dade North WWTP. Florida Keys sample sites 1 and 2 were located on Coffins Patch Reef. Attribute: Attribute_Label: tetB Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetL Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetM Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetO Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetW Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: ampC Attribute_Definition: Presence/absence of cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: vanA Attribute_Definition: Presence/absence of Vancomyucin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: ermB Attribute_Definition: Presence/absence of Erythromycin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: mecA Attribute_Definition: Presence/absence of Methicillin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: blaSHV Attribute_Definition: Presence/absence of Cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: blaPSE Attribute_Definition: Presence/absence of Cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: floR Attribute_Definition: Presence/absence of Chloramphenenicol resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: aadA2 Attribute_Definition: Presence/absence of Streptomycin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetG Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetQ Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Detailed_Description: Entity_Type: Entity_Type_Label: DrySeason_PCR_data_sheet.csv Entity_Type_Definition: Table containing attribute information associated with the dataset. Presence/absence qPCR data are provided in comma separated values format. Entity_Type_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: Sample type Attribute_Definition: Type of sample (sediment or water) collected at each sampling location. Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: Water samples were obtained from either the water column or the wastewater treatment plant tap water effluent. Attribute: Attribute_Label: Sample site Attribute_Definition: Sample site location Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: Sample sites included: Broward North WWTP, Broward North WWTP Outfall, Haµlover Outfall (Miami-Dade North), Hollywood Outfall, Hollywood WWTP, and Miami-Dade North WWTP. Attribute: Attribute_Label: tetB Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetL Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetM Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetO Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetW Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: ampC Attribute_Definition: Presence/absence of Cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: vanA Attribute_Definition: Presence/absence of Vancomyucin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: ermB Attribute_Definition: Presence/absence of Erythromycin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: mecA Attribute_Definition: Presence/absence of Methicillin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: blaSHV Attribute_Definition: Presence/absence of Cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: blaPSE Attribute_Definition: Presence/absence of Cephalosporin (ampicillin, penicillin, etc.) resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: floR Attribute_Definition: Presence/absence of Chloramphenenicol resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: aadA2 Attribute_Definition: Presence/absence of Streptomycin resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetG Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: tetQ Attribute_Definition: Presence/absence of Tetracycline resistance genetic target Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: {Null Value / Empty Field Entry} Enumerated_Domain_Value_Definition: Not detected Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: (+/+) Enumerated_Domain_Value_Definition: Present in two of two PCR reactions Enumerated_Domain_Value_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: Notes Attribute_Definition: Sample notes Attribute_Definition_Source: U.S. Geological Survey Attribute_Domain_Values: Unrepresentable_Domain: sed## = sediment sample, distance from outfall pipe in meters. (+/+) = two positive PCR reactions. IPC normal reaction was set at a Rn (normalized reporter) value of 0.1 with a CT (threshold cycle) of 28 plus or minus 1. Target reactions were judged positive if they breached the Rn of 0.1 with an exponential type signal in both reactions. {Null Value / Empty Field Entry} means no notes were recorded. Detailed_Description: Entity_Type: Entity_Type_Label: FLDEPSampleCollectionLog_DrySeason.csv, FLDEPSample CollectionLog_WetSeason.csv Entity_Type_Definition: Table containing attribute information associated with the dataset. Presence/absence qPCR data are provided in comma separated values format. Entity_Type_Definition_Source: U.S. Geological Survey Attribute: Attribute_Label: Collection Date (month/day/year) Attribute_Definition: Date samples were collected Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Sample dates in month/day/year format Attribute: Attribute_Label: Site Attribute_Definition: Sample site location Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Site location description Attribute: Attribute_Label: Sample/Site ID Attribute_Definition: Sample site identification Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Site identifier Attribute: Attribute_Label: Depth Attribute_Definition: Sample site depth, in feet Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Site depth Attribute: Attribute_Label: Water Temp. (F) Attribute_Definition: Sample site water temperature, in Fahrenheit Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Collection site water temperature Attribute: Attribute_Label: Weather Attribute_Definition: Sample site weather Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Site descriptions of weather conditions include: seas in feet, atmospheric condition, and wind speed in knots or miles per hour. Attribute: Attribute_Label: Notes Attribute_Definition: Sample site Attribute_Definition_Source: FLDEP Attribute_Domain_Values: Unrepresentable_Domain: Other metadata Overview_Description: Entity_and_Attribute_Overview: The entity and attribute information provided here describes the tabular data associated with the dataset. Please review the detailed descriptions that are provided (the individual attribute descriptions) for information on the values that appear as fields/table entries of the dataset. Entity_and_Attribute_Detail_Citation: The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information. Distribution_Information: Distributor: Contact_Information: Contact_Organization_Primary: Contact_Organization: U.S. Geological Survey Contact_Address: Address_Type: Physical and Mailing Address: 600 4th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: USA Contact_Voice_Telephone: 727-502-8000 Contact_Electronic_Mail_Address: dgriffin@usgs.gov Distribution_Liability: Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Standard_Order_Process: Digital_Form: Digital_Transfer_Information: Format_Name: comma-delimited text Format_Version_Number: None Digital_Transfer_Option: Online_Option: Computer_Contact_Information: Network_Address: Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P98KQWDN/data/WetSeason_PCR_data_sheet.zip Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P98KQWDN/data/DrySeason_PCR_data_sheet.zip Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P98KQWDN/data/FLDEPSampleCollectionLog_WetSeason.zip Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P98KQWDN/data/FLDEPSampleCollectionLog_DrySeason.zip Fees: None Metadata_Reference_Information: Metadata_Date: 20201013 Metadata_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Dale W. Griffin Contact_Organization: U.S. Geological Survey, Southeast Region Contact_Position: Environmental and Public Health Microbiologist Contact_Address: Address_Type: mailing address Address: 600 4Th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: USA Contact_Voice_Telephone: 727-502-8075 Contact_Facsimile_Telephone: 727-502-8182 Contact_Electronic_Mail_Address: dgriffin@usgs.gov Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata Metadata_Standard_Version: FGDC-STD-001-1998