Microbial and environmental dataset from Crocker Reef, Florida Keys, 2014-2015

Frequently anticipated questions:

• What does this data set describe?
  1. How might this data set be cited?
  2. What geographic area does the data set cover?
  3. What does it look like?
  4. Does the data set describe conditions during a particular time period?
  5. What is the general form of this data set?
  6. How does the data set represent geographic features?
  7. How does the data set describe geographic features?
• Who produced the data set?
  1. Who are the originators of the data set?
  2. Who also contributed to the data set?
  3. To whom should users address questions about the data?
• Why was the data set created?
• How was the data set created?
  1. From what previous works were the data drawn?
  2. How were the data generated, processed, and modified?
  3. What similar or related data should the user be aware of?
• How reliable are the data; what problems remain in the data set?
  1. How well have the observations been checked?
  2. How accurate are the geographic locations?
  3. How accurate are the heights or depths?
  4. Where are the gaps in the data? What is missing?
  5. How consistent are the relationships among the data, including topology?
• How can someone get a copy of the data set?
  1. Are there legal restrictions on access or use of the data?
  2. Who distributes the data?
  3. What's the catalog number I need to order this data set?
  4. What legal disclaimers am I supposed to read?
  5. How can I download or order the data?
• Who wrote the metadata?

What does this data set describe?

1. How might this data set be cited?
   Kellogg, Christina A., 20151105, Microbial and environmental dataset from Crocker Reef, Florida Keys, 2014-2015: U.S. Geological Survey Data Release doi:10.5066/F74Q7S25, U.S. Geological Survey, St. Petersburg, FL.

   Online Links:

   • https://doi.org/10.5066/F74Q7S25

   This is part of the following larger work.
   Kellogg, Christina A., Yates, Kimberly K., Lawler, Stephanie N., Moore, Christopher S., and Smiley, Nathan A., 20151104, Seasonal microbial and environmental parameters at Crocker Reef, Florida Keys, 2014-2015: U.S. Geological Survey Open-File Report 2015-1203, U.S. Geological Survey, Reston, VA.

   Online Links:

   • https://doi.org/10.3133/ofr20151203

2. What geographic area does the data set cover?

   West_Bounding_Coordinate: -80.52775
   East_Bounding_Coordinate: -80.52644
   North_Bounding_Coordinate: 24.91083
   South_Bounding_Coordinate: 24.90897
   

3. What does it look like?
4. Does the data set describe conditions during a particular time period?

   Beginning_Date: 08-Jul-2014

   Ending_Date: 05-Feb-2015

   Currentness_Reference:

   ground condition

5. What is the general form of this data set?

   Geospatial_Data_Presentation_Form: Tabular Digital Data
   

6. How does the data set represent geographic features?
   1. How are geographic features stored in the data set?
      This is a Point data set. It contains the following vector data types (SDTS terminology):
      • Entity point (94)
   2. What coordinate system is used to represent geographic features?
      Horizontal positions are specified in geographic coordinates, that is, latitude and longitude. Latitudes are given to the nearest 0.0197885540. Longitudes are given to the nearest 0.0227288676. Latitude and longitude values are specified in Decimal degrees. The ellipsoid used is WGS 1984.
      The semi-major axis of the ellipsoid used is 6378137.0.
      The flattening of the ellipsoid used is 1/298.257223563.
      
7. How does the data set describe geographic features?

   Crocker_datarelease_2014-2015.csv

   Microbial and environmental water column data from Crocker Reef, Florida, collected in 2014 and 2015. (Source: USGS)

   Sample_ID

   Sample identification code, includes site, date, and time sample was collected (Source: USGS) Sample identification code, includes site, date, and time sample was collected

   Year

   Year of data collection. (Source: USGS)

   Range of values
   Minimum:	2014
   Maximum:	2015

   Date

   Date of sample collection. (Source: USGS)

   Value	Definition
   10-Jul	Calendar date using day, month format
   11-Jul	Calendar date using day, month format
   12-Jul	Calendar date using day, month format
   13-Jul	Calendar date using day, month format
   2-Feb	Calendar date using day, month format
   3-Feb	Calendar date using day, month format
   30-Jan	Calendar date using day, month format
   31-Jan	Calendar date using day, month format
   4-Feb	Calendar date using day, month format
   8-Jul	Calendar date using day, month format
   9-Jul	Calendar date using day, month format

   Local_Time

   Time of sample collection, in local time. (Source: USGS)

   Value	Definition
   11:30	Local time using 24-hour clock convention
   15:30	Local time using 24-hour clock convention
   19:30	Local time using 24-hour clock convention
   23:30	Local time using 24-hour clock convention
   3:30	Local time using 24-hour clock convention
   7:30	Local time using 24-hour clock convention

   Time_Zone

   Time zone used during data collection. (Source: USGS)

   Value	Definition
   EDT	Eastern Daylight Time (EDT)
   EST	Eastern Standard Time (EST)

   Latitude

   Latitude of the sample location in decimal degrees (WGS84) (Source: USGS)

   Value	Definition
   24.90897 N	World Geodetic System 1984 Geographic Coordinate System
   24.90913 N	World Geodetic System 1984 Geographic Coordinate System
   24.91082 N	World Geodetic System 1984 Geographic Coordinate System
   24.91083 N	World Geodetic System 1984 Geographic Coordinate System

   Longitude

   Longitude of the sample location in decimal degrees (WGS84) (Source: USGS)

   Value	Definition
   80.52644 W	World Geodetic System 1984 Geographic Coordinate System
   80.52658 W	World Geodetic System 1984 Geographic Coordinate System
   80.52758 W	World Geodetic System 1984 Geographic Coordinate System
   80.52775 W	World Geodetic System 1984 Geographic Coordinate System

   Approximate Depth (m)

   Water depth at site in meters. Approximate because the water is shallow and affected by tides. (Source: USGS)

   Range of values
   Minimum:	3.7
   Maximum:	4.3

   Virus/mL

   Viral particles enumerated by flow cytometry per milliliter of seawater. (Source: USGS)

   Range of values
   Minimum:	1950000
   Maximum:	23300000

   Bacteria/mL

   Bacterial particles enumerated by flow cytometry per milliliter of seawater. (Source: USGS)

   Range of values
   Minimum:	241000
   Maximum:	826000

   DO

   Dissolved Oxygen (Source: USGS)

   Range of values
   Minimum:	5.25
   Maximum:	6.9

   pH

   Sample pH (Source: USGS)

   Range of values
   Minimum:	0
   Maximum:	8.12

   Temperature (°C)

   Sample temperature, in degrees Celsius (Source: USGS)

   Range of values
   Minimum:	22.2
   Maximum:	31.9

   Salinity

   Sample salinity (Source: USGS)

   Range of values
   Minimum:	36.30
   Maximum:	37.57

   Vibrio CFU/mL

   Agar plate counts of Vibrio bacterial colony-forming units per milliliter of seawater. (Source: USGS) Detectable count values ranged between 3.3 and 3060 CFU/mL. All other values were listed as: BLD - Below Limit of Detection, ND - Not determined or TNTC Too Numerous To Count.

   Entity_and_Attribute_Overview:

   The entity and attribute information provided here describes the tabular data associated with the dataset. Please review the detailed descriptions that are provided (the individual attribute descriptions) for information on the values that appear as fields/table entries of the dataset.

   Entity_and_Attribute_Detail_Citation:

   The entity and attribute information was generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.

Who produced the data set?

1. Who are the originators of the data set? (may include formal authors, digital compilers, and editors)
   • Christina A. Kellogg
2. Who also contributed to the data set?
3. To whom should users address questions about the data?
   Christina Kellogg

   U.S. Geological Survey

   600 4th Street S.

   St. Petersburg, Florida
   

   USA

   727-502-8128 (voice)

   ckellogg@usgs.gov

Why was the data set created?

Data were collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Data were acquired during USGS field activity number (FAN) 2014-315-FA and 2015-303-FA; additional survey details are available at https://cmgds.marine.usgs.gov/fan_info.php?fan=2014-315-FA and https://cmgds.marine.usgs.gov/fan_info.php?fan=2015-303-FA. This dataset, Crocker_datarelease_2014-2015.csv, is included in a USGS data release that can be accessed online at https://doi.org/10.5066/F74Q7S25.

How was the data set created?

1. From what previous works were the data drawn?
2. How were the data generated, processed, and modified?

   Date: 01-Mar-2015 (process 1 of 3)

   Data collected in the field were either analyzed in-situ or brought back to the St. Petersburg Coastal and Marine Science Center for subsequent lab analyses.

   Date: 29-May-2018 (process 2 of 3)

   Keywords section of metadata optimized by adding theme keyword thesauri and associated keywords. Person who carried out this activity:
   

   U.S. Geological Survey

   Attn: Arnell S. Forde

   Geologist

   600 4th Street South

   St. Petersburg, FL
   

   727-502-8000 (voice)

   aforde@usgs.gov

   Date: 13-Oct-2020 (process 3 of 3)

   Added keywords section with USGS persistent identifier as theme keyword. Person who carried out this activity:
   

   U.S. Geological Survey

   Attn: VeeAnn A. Cross

   Marine Geologist

   384 Woods Hole Road

   Woods Hole, MA
   

   508-548-8700 x2251 (voice)

   508-457-2310 (FAX)

   vatnipp@usgs.gov

3. What similar or related data should the user be aware of?

How reliable are the data; what problems remain in the data set?

1. How well have the observations been checked?
   A YSI multimeter was used to measure temperature (+/-0.01 C), salinity (+/-0.01) and dissolved oxygen (+/-0.1 mg l-1). Samples were analyzed for total pH (pHT; +/-0.005) using an Ocean Optics spectrometer and thymol blue indicator dye. Bacterial and viral counts are averages of triplicate sample counts made on flow cytometry. Vibrio counts are averages of triplicate TCBS agar plate counts.
2. How accurate are the geographic locations?
   No formal positional accuracy tests were conducted.
3. How accurate are the heights or depths?
   A formal accuracy assessment of the vertical positional information in the dataset has either not been conducted, or is not applicable.
4. Where are the gaps in the data? What is missing?
   Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
5. How consistent are the relationships among the observations, including topology?
   No formal logical accuracy tests were conducted.

How can someone get a copy of the data set?

Are there legal restrictions on access or use of the data?

Access_Constraints: none
Use_Constraints:

The U.S. Geological Survey requests to be acknowledged as originators of the data in future products or derivative research.

1. Who distributes the data set? (Distributor 1 of 1)
   
   Christina Kellogg

   U.S. Geological Survey

   600 4th Street S.

   St. Petersburg, Florida
   

   USA

   727-502-8128 (voice)

   ckellogg@usgs.gov
2. What's the catalog number I need to order this data set?
3. What legal disclaimers am I supposed to read?

   Although these data have been processed successfully on a computer system at the USGS, no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described and/or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

4. How can I download or order the data?
   • Availability in digital form:

     Data format:	CSV (Comma-Separated Values
     Network links:	https://coastal.er.usgs.gov/data-release/doi-F74Q7S25/data/Crocker_datarelease_2014-2015.zip

   • Cost to order the data: None. No fees are applicable for obtaining the dataset.

Who wrote the metadata?

Dates:

Last modified: 13-Oct-2020

Metadata author:

Christina Kellogg

U.S. Geological Survey

600 4th Street S.

St. Petersburg, Florida

USA

727-502-8128 (voice)

ckellogg@usgs.gov

Metadata standard:

Content Standard for Digital Geospatial Metadata (FGDC-STD-001-1998)