Metadata: Identification_Information: Citation: Citation_Information: Originator: Leila Chapron Originator: Ilsa B. Kuffner Originator: Elise F. Keister Originator: Lucy A. Bartlett Originator: Andrea G. Grottoli Publication_Date: 20230717 Title: Experimental coral-physiology data for Acropora palmata in Florida, U.S.A. Geospatial_Data_Presentation_Form: tabular digital data Larger_Work_Citation: Citation_Information: Originator: Leila Chapron Originator: Ilsa B. Kuffner Originator: Dustin W. Kemp Originator: Ann M. Hulver Originator: Elise F. Keister Originator: Anastasios Stathakopoulos Originator: Lucy A. Bartlett Originator: Erin O. Lyons Originator: Andrea G. Grottoli Publication_Date: 20230717 Title: Experimental Coral-Physiology Data for Acropora palmata in Florida, USA Series_Information: Series_Name: U.S. Geological Survey data release Issue_Identification: doi:10.5066/P9FIBAKX Publication_Information: Publication_Place: St. Petersburg, Florida Publisher: U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center Other_Citation_Details: 2023a Online_Linkage: https://doi.org/10.5066/P9FIBAKX Description: Abstract: The U.S. Geological Survey (USGS) Coral Reef Ecosystems Studies (CREST) project (https://coastal.er.usgs.gov/crest/) provides science that helps Department of Interior and other resource managers tasked with the stewardship of coral reef resources. This data release contains data on coral physiology of the elkhorn coral, Acropora palmata, grown at five sites along the Florida outer reef tract including in Biscayne National Park, the Florida Keys National Marine Sanctuary, and Dry Tortugas National Park, from summer 2017 to autumn 2020. The data will be used to inform resource managers of the capacity for restoration and growth of this important, habitat-forming species of coral within U.S. waters. Some datasets included here were interpreted in Chapron and others (2023b). Chapron, L., Kuffner, I.B., Kemp, D.W., Hulver, A.M., Keister, E.F., Stathakopoulos, A., Bartlett, L.A., Lyons, E.O., and Grottoli, A.G., 2023, Heterotrophy, microbiome, and location effects on restoration efficacy of the threatened coral Acropora palmata: Communications Earth and Environment, vol. 4, art. 233, https://doi.org/10.1038/s43247-023-00888-1. Purpose: These data were obtained to directly quantify a suite of coral physiological variables in surviving Acropora palmata corals from Kuffner and others (2020a) on the Florida Keys reef tract and Dry Tortugas National Park. The data will be used to inform resource managers of the capacity for future restoration and growth of this species on Florida Keys reefs to guide coral-reef ecosystem and species-recovery plans. Supplemental_Information: Scientific research permits: The data herein were collected with permission from the National Park Service (NPS) and the Florida Keys National Marine Sanctuary (FKNMS) under permit numbers BISC-2018-SCI-0014, BISC-2019-SCI-0010, BISC-2020-SCI-0013, DRTO-2017-SCI-0001, DRTO-2019-SCI-0005, FKNMS-2016-085-A1 and FKNMS-2019-139-A1. To ensure that USGS-St. Petersburg data management protocols were followed, each survey when data were collected were assigned the following USGS field activity numbers (FANs): 2017-311-FA, 2017-352-FA, 2018-327-FA, 2018-359-FA, 2019-316-FA, 2019-367-FA, and 2020-307-FA. Additional survey and data details are available from the U.S. Geological Survey Coastal and Marine Geoscience Data System (CMGDS) at, https://cmgds.marine.usgs.gov/. Time_Period_of_Content: Time_Period_Information: Range_of_Dates/Times: Beginning_Date: 201704 Ending_Date: 202011 Currentness_Reference: ground condition Status: Progress: Complete Maintenance_and_Update_Frequency: None planned Spatial_Domain: Bounding_Coordinates: West_Bounding_Coordinate: -82.79883 East_Bounding_Coordinate: -80.09560 North_Bounding_Coordinate: 25.59047 South_Bounding_Coordinate: 24.62687 Keywords: Theme: Theme_Keyword_Thesaurus: USGS Metadata Identifier Theme_Keyword: USGS:3dad184c-78b3-4ccb-a1af-6eb437331345 Theme: Theme_Keyword_Thesaurus: USGS Thesaurus Theme_Keyword: aquatic biology Theme_Keyword: marine geology Theme_Keyword: coelenterates Theme_Keyword: ocean temperature Theme_Keyword: sea-floor characteristics Theme_Keyword: marine biology Theme_Keyword: biochemistry Theme_Keyword: physiological processes Theme_Keyword: algae Theme_Keyword: time series datasets Theme: Theme_Keyword_Thesaurus: ISO 19115 Topic Category Theme_Keyword: environment Theme_Keyword: geoscientificInformation Theme_Keyword: oceans Theme: Theme_Keyword_Thesaurus: None Theme_Keyword: coral reef Theme_Keyword: coral Theme_Keyword: Acropora Theme_Keyword: calcification Theme_Keyword: carbonate budget Theme_Keyword: heterotrophy Theme_Keyword: Symbiodiniaceae Theme_Keyword: coral physiology Theme_Keyword: lipid classes Theme_Keyword: trophic studies Place: Place_Keyword_Thesaurus: Geographic Names Information System Place_Keyword: Florida Keys Place_Keyword: Florida Place_Keyword: Monroe County Place_Keyword: Atlantic Ocean Place_Keyword: Islamorada Stratum: Stratum_Keyword_Thesaurus: None Stratum_Keyword: seafloor Temporal: Temporal_Keyword_Thesaurus: None Temporal_Keyword: Modern Access_Constraints: None Use_Constraints: Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey requests to be acknowledged as originator of these data in future products or derivative research. Point_of_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Ilsa B. Kuffner Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center Contact_Position: Research Marine Biologist Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: U.S.A. Contact_Voice_Telephone: 727-502-8048 Contact_Electronic_Mail_Address: ikuffner@usgs.gov Data_Set_Credit: Acknowledgment of Ilsa B. Kuffner and the U.S. Geological Survey as data sources would be appreciated in products developed from these data, and such acknowledgment as is standard for citation and legal practices for data source is expected. Native_Data_Set_Environment: Environment as of Metadata Creation: Microsoft Windows 10 Version 21H2 (Build 19044.1706) Windows Feature Experience Pack 120.2212.4170.0 Cross_Reference: Citation_Information: Originator: Chapron, L., Kuffner, I.B., Kemp, D.W., Hulver, A.M., Keister, E.F., Stathakopoulos, A., Bartlett, L.A., Lyons, E.O., and Grottoli, A.G. Publication_Date: 20230717 Title: Heterotrophy, microbiome, and location effects on restoration efficacy of the threatened coral Acropora palmata Publication_Information: Publication_Place: Online Publisher: Nature: Communications Earth and Environment Other_Citation_Details: 2023b Online_Linkage: https://doi.org/10.1038/s43247-023-00888-1 Cross_Reference: Citation_Information: Originator: Jokiel, P.L., Maragos, J.E., and Franzisket, L. Publication_Date: 1978 Title: Coral growth: buoyant weight technique Publication_Information: Publication_Place: Paris, France Publisher: Coral Reefs: Research Methods Other_Citation_Details: Editors Stoddart, D.R., and Johannes, R.E., pages 529-541 Cross_Reference: Citation_Information: Originator: Morrison, J.M., Kuffner, I.B., and Hickey, T.D. Publication_Date: 2013 Title: Methods for monitoring corals and crustose coralline algae to quantify in-situ calcification rates Series_Information: Series_Name: U.S. Geological Survey Open-File Report Issue_Identification: 2013-1159 Publication_Information: Publication_Place: Reston, Virginia Publisher: U.S. Geological Survey Other_Citation_Details: 11 pages Online_Linkage: https://doi.org/10.3133/ofr20131159 Cross_Reference: Citation_Information: Originator: Kuffner, I.B., Stathakopoulos, A., Toth, L.T., and Bartlett, L.A. Publication_Date: 20201217 Title: Reestablishing a stepping-stone population of the threatened elkhorn coral Acropora palmata to aid regional recovery Publication_Information: Publication_Place: Germany Publisher: Endangered Species Research Other_Citation_Details: 2020a, Volume 43, pages 461-473 Online_Linkage: https://doi.org/10.3354/esr01083 Cross_Reference: Citation_Information: Originator: Kuffner, I.B., Stathakopolous, A., Toth, L.T., and Bartlett L.A. Publication_Date: 20201013 Title: Experimental coral-growth data and time-series imagery for Acropora palmata in the Florida Keys, U.S.A. Series_Information: Series_Name: U.S. Geological Survey data release Issue_Identification: doi:10.5066/P9KZEGXY Publication_Information: Publication_Place: St. Petersburg, Florida Publisher: U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center Other_Citation_Details: 2020b Online_Linkage: https://doi.org/10.5066/P9KZEGXY Cross_Reference: Citation_Information: Originator: Marsh, J.A. Jr. Publication_Date: 19700301 Title: Primary productivity of reef-building calcareous red algae Publication_Information: Publication_Place: Washington, D.C. Publisher: Ecology Other_Citation_Details: Volume 51, Issue 2, pages 255-263 Online_Linkage: https://doi.org/10.2307/1933661 Cross_Reference: Citation_Information: Originator: Mclachlan, R., Dobson, K., and Grottoli, A.G. Publication_Date: 20200422 Title: Quantification of total biomass in ground coral samples v1 Publication_Information: Publication_Place: Online Publisher: protocols.io Other_Citation_Details: 2020a Online_Linkage: https://doi.org/10.17504/protocols.io.bdyai7se Cross_Reference: Citation_Information: Originator: Mclachlan, R., Juracka, C., and Grottoli, A.G. Publication_Date: 20200316 Title: Symbiodiniaceae enumeration in ground coral samples Using CountessTM II FL Automated Cell Counter v1 Publication_Information: Publication_Place: Online Publisher: protocols.io Other_Citation_Details: 2020b Online_Linkage: https://doi.org/10.17504/protocols.io.bdc5i2y6 Cross_Reference: Citation_Information: Originator: Mclachlan, R., Munoz-Garcia, A., and Grottoli, A.G. Publication_Date: 20200313 Title: Primary extraction of total soluble lipid from ground coral samples v1 Publication_Information: Publication_Place: Online Publisher: protocols.io Other_Citation_Details: 2020c Online_Linkage: https://doi.org/10.17504/protocols.io.bc4qiyvw Cross_Reference: Citation_Information: Originator: Jeffrey, S.T., and Humphrey, G.F. Publication_Date: 1975 Title: New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton Publication_Information: Publication_Place: ScienceDirect Publisher: Biochemie und Physiologie der Pflanzen Other_Citation_Details: Volume 167, Issue 2, pages 191-194 Online_Linkage: https://doi.org/10.1016/S0015-3796(17)30778-3 Cross_Reference: Citation_Information: Originator: Bradford, M.M. Publication_Date: 19760129 Title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Publication_Information: Publication_Place: Amsterdam, Netherlands Publisher: Analytical Biochemistry Other_Citation_Details: Volume 72, Issues 1-2, pages 248-254 Online_Linkage: https://doi.org/10.1016/0003-2697(76)90527-3 Cross_Reference: Citation_Information: Originator: Gnaiger, E., and Bitterlich, G. Publication_Date: 198406 Title: Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept Publication_Information: Publication_Place: Berlin, Germany Publisher: Oecologia Other_Citation_Details: Volume 62, pages 289-298 Online_Linkage: https://doi.org/10.1007/BF00384259 Cross_Reference: Citation_Information: Originator: Price, J., Smith, A., Dobson, K., and Grottoli, A.G. Publication_Date: 20200709 Title: Airbrushed coral sample preparation for organic stable carbon and nitrogen isotope analyses v1 Publication_Information: Publication_Place: Online Publisher: protocols.io Online_Linkage: https://doi.org/10.17504/protocols.io.bgi7juhn Cross_Reference: Citation_Information: Originator: Price, J.T., McLachlan, R.H., Jury, C.P., Toonen, R.J., and Grottoli, A.G. Publication_Date: 20210506 Title: Isotopic approaches to estimating the contribution of heterotrophic sources to Hawaiian corals Publication_Information: Publication_Place: Online Publisher: Limnology and Oceanography Other_Citation_Details: Volume 66, Issue 6, pages 2393–2407 Online_Linkage: https://doi.org/10.1002/lno.11760 Cross_Reference: Citation_Information: Originator: Rodrigues, L.J., and Grottoli, A.G. Publication_Date: 20060601 Title: Calcification rate and the stable carbon, oxygen, and nitrogen isotopes in the skeleton, host tissue, and zooxanthellae of bleached and recovering Hawaiian corals Publication_Information: Publication_Place: Amsterdam, Netherlands Publisher: Geochimica et Cosmochimica Acta Other_Citation_Details: Volume 70, Issue 11, pages 2781–2789 Online_Linkage: https://doi.org/10.1016/j.gca.2006.02.014 Cross_Reference: Citation_Information: Originator: Conlan, J.A., Jones, P.L., Turchini, G.M., Hall, M.R., and Francis, D.S. Publication_Date: 20141020 Title: Changes in the nutritional composition of captive early-mid stage Panulirus ornatus phyllosoma over ecdysis and larval development Publication_Information: Publication_Place: Amsterdam, Netherlands Publisher: Aquaculture Other_Citation_Details: Volume 434, pages 159-170 Online_Linkage: https://doi.org/10.1016/j.aquaculture.2014.07.030 Data_Quality_Information: Attribute_Accuracy: Attribute_Accuracy_Report: No formal attribute accuracy tests were conducted. Logical_Consistency_Report: No formal logical accuracy tests were conducted. Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Missing values are marked “NA” for “no analyses,” as measurements for these variables were not possible because of insufficient coral tissue available for analysis. Positional_Accuracy: Horizontal_Positional_Accuracy: Horizontal_Positional_Accuracy_Report: No formal positional accuracy tests were conducted. Vertical_Positional_Accuracy: Vertical_Positional_Accuracy_Report: No formal positional accuracy tests were conducted. Lineage: Process_Step: Process_Description: Coral collection and experimental design- Acropora palmata genets were originally sourced from different upper Florida Keys reefs by the Coral Restoration Foundation (CRF) prior to being cultivated in CRF’s offshore coral nurseries. The forty-one corals sampled in the study were replicates (ramets) from six unique genets (CF4, CN2, HS1, ML2, SN1, and DT1), which were mounted on labeled Polyvinyl chloride (PVC discs), buoyantly weighed (Jokiel and others, 1978), and deployed on fixed, concrete stations at five USGS calcification-assessment-network sites along the Florida Keys reef tract (Morrison and others, 2013). These sites, from west to east, were Pulaski West and Pulaski Light in Dry Tortugas National Park, Sombrero Reef and Crocker Reef in the Florida Keys National Marine Sanctuary, and Fowey Rocks in Biscayne National Park. Thirty-five surviving ramets from an assisted-migration experiment initiated in Spring of 2018 (Kuffner and others 2020b) were sampled after the end of that study in the Autumn of 2020. To increase sample size, six additional ramets were sampled at that time (from three of the same CRF-nursery genets) that had been deployed at Crocker Reef in 2017, for a discontinued experiment that was interrupted by Hurricane Irma. Generally, n = 2 ramets from each of the five upper Florida Keys genets were placed at each of the five sites, and an additional n = 2 ramets from the Dry Tortugas genet (DT1) were placed at the two Dry Tortugas sites. Ramets sourced from the Dry Tortugas genet were not reciprocally transplanted to the main Florida Keys sites because of permitting and logistical restrictions. As part of the assisted-migration study, calcification rates, linear extension, and planar area of the translocated corals were measured every six months from May 2018 to October 2019 and are reported elsewhere (Kuffner and others, 2020a; 2020b). Process_Date: 2020 Process_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Ilsa B. Kuffner Contact_Organization: Southeast Region Contact_Position: Research Marine Biologist Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8048 Contact_Electronic_Mail_Address: ikuffner@usgs.gov Process_Step: Process_Description: Tissue biomass, endosymbiont density, chlorophyll a, and energy reserves- The surface area of the ramet section designated for physiological analyses was determined using the aluminum foil method (Marsh, 1970). Then, ground to a homogeneous paste (skeleton, host, and endosymbiont) and divided into 5 sub-samples, each used for individual physiological analysis. Tissue biomass was measured using methods described by Mclachlan and others (2020a). Endosymbiont density was measured using 4 replicated counts on a Countesso II FL Automated Cell Counter (Mclachlan and others, 2020b). Chlorophyll a was extracted using a double 100% acetone extraction and quantified with a spectrophotometer using the equation from Jeffrey and Humphrey (1975). Tissue biomass, endosymbiont density, and chlorophyll a were standardized to surface area based on the proportional representation of each of their respective sub-samples. Total lipids were extracted using a chloroform:methanol solution and proteins were extracted using the Bio-Rad© protein assay solution following established methods of Mclachlan and others, (2020c) and Bradford (1976), respectively. The energetic value of total lipids and proteins concentrations were reported in Joules (Gnaiger and Bitterlich, 1984), and standardized to ash-free dry weight of the coral tissue. Process_Date: 2021 Process_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Andréa G. Grottoli Contact_Organization: The Ohio State University Contact_Position: Professor of Earth Sciences Contact_Address: Address_Type: mailing and physical Address: 275 Mendenhall Laboratory, 125 South Oval Mall City: Columbus State_or_Province: OH Postal_Code: 43210 Country: United States Contact_Voice_Telephone: 614-292-5782 Contact_Electronic_Mail_Address: grottoli.1@osu.edu Process_Step: Process_Description: Stable isotopic analysis- Isotopic analyses were assessed on separated animal host and algal endosymbiont fractions using methods described in Price and others, (2020). From the ramet section designated for isotopic analyses, between 4 to 8 square centimeters (cm2) of coral tissue was removed using an airbrush, and the host and endosymbiont fractions were separated by centrifugation and filtration. Each sample was individually combusted using a PDZ Europa ANCA-GSL element analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheschier, UK) at the University of California Davis Stable Isotope Facility. The carbon (C) and nitrogen (N) isotopic composition of the animal host (δ13Ch, δ15Nh) and algal endosymbiont (δ13Ce, δ15Ne) were reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna-Peedee Belemnite Limestone Standard (v-PDB) and 15N:14N relative to air, respectively. Repeated measurements of internal standards had a standard deviation of ±0.2‰ and ±0.23‰ for δ13C and δ15N, respectively. The difference between δ13Ch and δ13Ce (δ13C-e) and the δ15Nh and δ15Ne (δ15N-e) both represent the relative contribution of photoautotrophic versus heterotrophic carbon to coral tissues (Price and others, 2021; Rodrigues and Grottoli, 2006). Process_Date: 2021 Process_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Andréa G. Grottoli Contact_Organization: The Ohio State University Contact_Position: Professor of Earth Sciences Contact_Address: Address_Type: mailing and physical Address: 275 Mendenhall Laboratory, 125 South Oval Mall City: Columbus State_or_Province: OH Postal_Code: 43210 Country: United States Contact_Voice_Telephone: 614-292-5782 Contact_Electronic_Mail_Address: grottoli.1@osu.edu Process_Step: Process_Description: Lipid class analyses- The total lipids were dried under nitrogen gas over a water bath at 55 degrees Celsius (°C). The vials containing the dried total lipids were frozen at -80°C and transported on dry ice to the University of Alabama in Birmingham for lipid class analysis. The dried total lipids were resuspended in 100% chloroform at a concentration of 10 milligrams per milliliter (mg/mL). Lipid class composition was analyzed by an Iatroscan MK 6S thin layer flame ionization detector (NTS) according to the methods modified from Conlan and others, (2014). Briefly, 1 microliter (µL) of the solution, in duplicate, was spotted onto each thin layer quartz-impregnated rod (Chromarods Mitsubishi Kagaku Iatron, Inc.) of the Iatroscan rack using a metal and glass syringe. The rack, containing ten loaded rods was placed in the first closed development chamber containing 50:20:1 (v:v:v) chloroform:methanol:Milli-Q and developed to half height (~7 minutes). The rack was then dried for two minutes at room temperature and transferred to the second closed development chamber containing 57:14.2:1.4 (v:v:v) hexane:ethyl ether:formic acid and developed to full height (~17 minutes). The rack of rods was dried in the oven at 100°C for 10 minutes prior to being scanned, using flame ionization detection with an Iastroscan MK 6S detector (Air pressure at 2, Hydrogen flow at 165 mL/minute). The amount of each lipid class (wax esters, triacylglycerol, sterols, acetone mobile polar lipids, and phospholipids) on each rod was determined by comparison to a standard curve produced from 1 microliter (µL) standards of known concentrations for each lipid class, ranging from 0.078 mg/mL to 10 mg/mL. Each lipid class concentration was normalized to the amount of total lipid initially extracted and standardized to ash-free dry weight for each sample. Each sample was run in duplicate, and the results of the duplicate runs averaged to produce one lipid class value for each coral ramet sample. Process_Date: 2021 Process_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Andréa G. Grottoli Contact_Organization: The Ohio State University Contact_Position: Professor of Earth Sciences Contact_Address: Address_Type: mailing and physical Address: 275 Mendenhall Laboratory, 125 South Oval Mall City: Columbus State_or_Province: OH Postal_Code: 43210 Country: United States Contact_Voice_Telephone: 614-292-5782 Contact_Electronic_Mail_Address: grottoli.1@osu.edu Spatial_Data_Organization_Information: Direct_Spatial_Reference_Method: Point Point_and_Vector_Object_Information: SDTS_Terms_Description: SDTS_Point_and_Vector_Object_Type: Point Point_and_Vector_Object_Count: 41 Spatial_Reference_Information: Horizontal_Coordinate_System_Definition: Geographic: Latitude_Resolution: 0.000000001 Longitude_Resolution: 0.000000001 Geographic_Coordinate_Units: Decimal Degrees Geodetic_Model: Horizontal_Datum_Name: WGS_1984 Ellipsoid_Name: WGS_84 Semi-major_Axis: 6378137.0 Denominator_of_Flattening_Ratio: 298.257223563 Entity_and_Attribute_Information: Detailed_Description: Entity_Type: Entity_Type_Label: Palmata_physiology_FL_USA.csv, Palmata_physiology_FL_USA.xlsx Entity_Type_Definition: These files contain attribute information for data describing the physiology of corals of the species Acropora palmata, which were obtained from the Coral Restoration Foundation (Key Largo, Florida) and grown at five study sites on the Florida Keys reef tract. Corals were deployed in Spring of 2018 and samples extracted in Autumn 2020. Data include location information, sample identifiers, tissue biomass, endosymbiont density, chlorophyll a, isotopic analyses, and lipid analyses. The data is available as a comma-separated values (.csv) file and a Microsoft Excel (.xlsx) file. Missing values are marked “NA” for “no analyses,” as measurements for these variables were not possible because of insufficient coral tissue available for analysis. Entity_Type_Definition_Source: USGS Attribute: Attribute_Label: Location Attribute_Definition: A textual identifier of the site, assigned by locations where corals were deployed and named by USGS personnel. Attribute_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: Pulaski West Enumerated_Domain_Value_Definition: Corals were deployed at Pulaski West (12 feet of seawater [fsw]), in Dry Tortugas National Park, Florida. Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: Pulaski Light Enumerated_Domain_Value_Definition: Corals were deployed at Pulaski Light (16 fsw), in Dry Tortugas National Park, Florida. Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: Sombrero Reef Enumerated_Domain_Value_Definition: Corals were deployed at Sombrero Reef (14 fsw), in the Sanctuary Preservation Area of the FKNMS, Florida. Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: Crocker Reef Enumerated_Domain_Value_Definition: Corals were deployed at Crocker Reef (13 fsw), in the FKNMS, Florida. Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: Fowey Rocks Enumerated_Domain_Value_Definition: Corals were deployed at Fowey Rocks (13 fsw), in Biscayne National Park, Florida. Enumerated_Domain_Value_Definition_Source: USGS Attribute: Attribute_Label: Latitude Attribute_Definition: Latitudinal coordinate (in the World Geodetic System of 1984 [WGS 84] coordinate system) of the site locations where corals were deployed by USGS personnel. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 24.62687 Range_Domain_Maximum: 25.59047 Attribute_Units_of_Measure: decimal degrees Attribute: Attribute_Label: Longitude Attribute_Definition: Longitudinal coordinate (in the WGS 84 coordinate system) of the site locations where corals were deployed by USGS personnel. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: -82.79883 Range_Domain_Maximum: -80.09560 Attribute_Units_of_Measure: decimal degrees Attribute: Attribute_Label: Coral_Genet Attribute_Definition: A textual identifier for each genetic strain (genet) of elkhorn coral in the study, as assigned by Coral Reef Foundation (Key Largo, FL) personnel, or the USGS (Dry Tortugas genet only). This identifier is based upon the collection location of the original colony from which samples were taken and placed into nursery propagation. The two-letter site abbreviations are "DT" indicates Dry Tortugas, "CN" indicates Conch Reef, "SN" indicates Snapper Ledge, "CF" indicates Carysfort Reef, "HS" indicates Horseshoe Reef, and "ML" indicates Molasses Reef. The collection locations of these colonies are detailed in Figure 1 from Kuffner and others (2020a). Attribute_Definition_Source: USGS Attribute_Domain_Values: Unrepresentable_Domain: A textual description of the geographic site where the original coral genets were collected by the Coral Restoration Foundation (not the site where the fragments were deployed). Attribute: Attribute_Label: Coral_ID Attribute_Definition: A numeric identifier for each coral colony in the study, assigned by USGS personnel. Attribute_Definition_Source: USGS Attribute_Domain_Values: Unrepresentable_Domain: Non-sequential, increasing numerical identifiers ranging between 13 and 558. Attribute: Attribute_Label: Biomass_mg_cm2 Attribute_Definition: Tissue biomass was measured using methods described by Mclachlan and others (2020a). Attribute_Definition_Source: Ohio State University (OSU) Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 2.56 Range_Domain_Maximum: 13.76 Attribute_Units_of_Measure: milligrams per square centimeter Attribute: Attribute_Label: Total_lipids_kJ_gdw Attribute_Definition: Total lipids extracted using a chloroform:methanol solution following established methods of Bradford (1976). The energetic value of total lipids concentration was reported in kilojoules (kJ) (Gnaiger and Bitterlich, 1984) and standardized to ash-free dry weight of the coral tissue. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 2.25 Range_Domain_Maximum: 12.89 Attribute_Units_of_Measure: kilojoules per grams of ash-free dry weight of coral tissue Attribute: Attribute_Label: Total_Protein_J_gdw Attribute_Definition: Total proteins extracted using the Bio-Rad protein assay solution following established methods of Mclachlan and others, (2020c) and standardized to ash-free dry weight of the coral tissue. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 24.73 Range_Domain_Maximum: 69.81 Attribute_Units_of_Measure: joules per grams of ash-free dry weight of coral tissue Attribute: Attribute_Label: Symbiodiniaceae_cells_cm2 Attribute_Definition: Endosymbiont density measured using four replicated counts on a Countess II FL Automated Cell Counter (Mclachlan and others, 2020b) and standardized to surface area based on its proportional representation of the tissue biomass. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 593779 Range_Domain_Maximum: 4400303 Attribute_Units_of_Measure: cells per square centimeter of coral skeleton Attribute: Attribute_Label: Chl_a_ug_cm2 Attribute_Definition: Chlorophyll a was extracted using a double 100% acetone extraction and quantified with a spectrophotometer using the equation from Jeffrey and Humphrey (1975) and standardized to surface area based on its proportional representation of the tissue biomass. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 184.31 Range_Domain_Maximum: 2038.29 Attribute_Units_of_Measure: micrograms per square centimeter of coral skeleton Attribute: Attribute_Label: d13C_endo_ppt Attribute_Definition: The carbon isotopic composition of the algal endosymbiont (δ13Ce) reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna-Peedee Belemnite Limestone Standard (v-PDB). Repeated measurements of internal standards had a standard deviation of ±0.2‰ for δ13C. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: -23.25 Range_Domain_Maximum: -10.36 Attribute_Units_of_Measure: per mil deviation of the stable isotopes 13C:12C relative to Vienna-Peedee Belemnite Limestone Standard (v-PDB) Attribute: Attribute_Label: d15N_endo_ppt Attribute_Definition: The nitrogen isotopic composition of the algal endosymbiont (δ15Ne) reported as the per mil deviation of the stable isotopes 15N:14N relative to air. Repeated measurements of internal standards had a standard deviation of ±0.23‰ for δ15N. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 1.09 Range_Domain_Maximum: 3.73 Attribute_Units_of_Measure: per mil deviation of the stable isotopes 15N:14N relative to air Attribute: Attribute_Label: d13C_host_ppt Attribute_Definition: The carbon isotopic composition of the animal host (δ13Ch) reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna-Peedee Belemnite Limestone Standard (v-PDB). Repeated measurements of internal standards had a standard deviation of ±0.2‰ δ13C. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: -18.85 Range_Domain_Maximum: -14.63 Attribute_Units_of_Measure: per mil deviation of the stable isotopes 13C:12C relative to Vienna-Peedee Belemnite Limestone Standard (v-PDB) Attribute: Attribute_Label: d15N_host_ppt Attribute_Definition: The nitrogen isotopic composition of the animal host (δ15Nh) reported as the per mil deviation of the stable isotopes 15N:14N relative to air. Repeated measurements of internal standards had a standard deviation of ±0.23‰ for δ15N. Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 2.32 Range_Domain_Maximum: 4.16 Attribute_Units_of_Measure: per mil deviation of the stable isotopes 15N:14N relative to air Attribute: Attribute_Label: Wax_mg_gdw Attribute_Definition: Wax esters in milligrams per gram of ash-free dry weight of coral tissue Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 5.83 Range_Domain_Maximum: 590.09 Attribute_Units_of_Measure: milligrams per gram of ash-free dry weight of coral tissue Attribute: Attribute_Label: TAG_mg_gdw Attribute_Definition: Triacylglycerol in milligrams per gram of ash-free dry weight of coral tissue Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 1.76 Range_Domain_Maximum: 24.05 Attribute_Units_of_Measure: milligrams per gram of ash-free dry weight of coral tissue Attribute: Attribute_Label: Sterol_mg_gdw Attribute_Definition: Sterols in milligrams per gram of ash-free dry weight of coral tissue Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 2.57 Range_Domain_Maximum: 20.76 Attribute_Units_of_Measure: milligrams per gram of ash-free dry weight of coral tissue Attribute: Attribute_Label: AMPL_mg_gdw Attribute_Definition: Acetone mobile polar lipids (AMPL) in milligrams per gram of ash-free dry weight of coral tissue Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.17 Range_Domain_Maximum: 5.94 Attribute_Units_of_Measure: milligrams per gram of ash-free dry weight of coral tissue Attribute: Attribute_Label: Phospholip_mg_gdw Attribute_Definition: Phospholipids in milligrams per gram of ash-free dry weight of coral tissue Attribute_Definition_Source: OSU Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 14.83 Range_Domain_Maximum: 150.78 Attribute_Units_of_Measure: milligrams per gram of ash-free dry weight of coral tissue Distribution_Information: Distributor: Contact_Information: Contact_Person_Primary: Contact_Person: Ilsa B. Kuffner Contact_Organization: U.S. Geological Survey Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: Saint Petersburg State_or_Province: FL Postal_Code: 33701 Country: U.S.A. Contact_Voice_Telephone: 727-502-8048 Contact_Electronic_Mail_Address: ikuffner@usgs.gov Distribution_Liability: This publication was prepared by an agency of the United States Government. Although these data have been processed successfully on a computer system at the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data on any other system, nor shall the act of distribution imply any such warranty. The U.S. Geological Survey shall not be held liable for improper or incorrect use of the data described and (or) contained herein. 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Metadata_Reference_Information: Metadata_Date: 20230717 Metadata_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Ilsa B. Kuffner Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center Contact_Position: Research Marine Biologist Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: U.S.A. Contact_Voice_Telephone: 727-502-8048 Contact_Electronic_Mail_Address: ikuffner@usgs.gov Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata Metadata_Standard_Version: FGDC-STD-001-1998