Kimberly Yates Christopher Moore Nathan Goldstein 20171004 Tabular Digital Data U.S. Geological Survey Data Release doi:10.5066/F7Q23XSF St. Petersburg, FL U. S. Geological Survey https://doi.org/10.5066/F7Q23XSF This dataset contains carbonate system data collected by scientists from the U.S. Geological Survey (USGS) St. Petersburg Coastal and Marine Science Center to investigate the effects of carbon cycling, coastal and ocean acidification on the Tampa Bay estuary located in west central Florida. Discrete seawater samples were collected along spatial transects at one to four hour intervals over 24-hour time periods. Water samples were analyzed at the USGS Carbon Analytical Laboratory in St. Petersburg Florida. This data set contains time series measurements of carbonate system parameters including: water temperature (Celsius, C), salinity, dissolved oxygen (milligrams/L), total alkalinity (TA, micromoles/kg), dissolved inorganic carbon (DIC, micromoles/kg), pHT (pH on the total scale), nitrate + nitrite (NO3+NO2, micromols/L), nitrite (NO2, micromols/L), silicate (SIL, micromols/L), ammonium (NH4, micromols/L) and phosphate (PO4, micromols/L). This dataset was acquired to assist USGS staff with the quantification, analysis and interpretation of geochemical ​time series data that will ultimately be used in assessments ​and ​research ​of or related to​ carbon cycling, coastal and ocean acidification. 201505 201601 ground condition Complete As needed -82.6937 -82.55393 27.96125 27.63571 USGS Metadata Identifier USGS:e1e74366-5bd5-409f-99b4-c2704127c394 None Salinity Dissolved oxygen Water temperature pH Total alkalinity Dissolved inorganic carbon Nitrite Ammonium Phosphate Silicate Nitrate Carbonate Seafloor Coastal acidification Nutrients Ocean acidification Lower Tampa Bay Middle Tampa Bay Upper Tampa Bay ISO 19115 Topic Category GeoscientificInformation Environment USGS Thesaurus Geochemistry Time series analysis Oxygen content (water) Geographic Names Information System (GNIS) Florida Tampa Bay Old Tampa Bay None Seafloor None Anthropocene Diurnal Diel Seasonal Annual Interannual none Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey requests to be acknowledged as originators of the data in future products or derivative research. Measured TA in this dataset may not be suitable to use for calculating carbonate system parameters, due to the potential influence of organic alkalinity on these values. Kimberly Yates United States Geological Survey mailing and physical

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St. Petersburg FL 33701 USA 7275028059 kyates@usgs.gov Acknowledgment of the USGS St. Petersburg Coastal and Marine Science Center, as a data source would be appreciated in products developed from these data, and such acknowledgment as is standard for citation and legal practices for data source is expected. Sharing of new data layers developed directly from these data would also be appreciated by the USGS staff. Accuracy of TA and DIC measurements is determined by measuring certified reference materials (CRMs) supplied by SCRIPPS Institute of Oceanography (Andrew Dickson) and comparing measured to known values. CRM analyses are performed daily before and after analysis of samples. Sample values are corrected to CRMs using a correction factor based on the difference between the known and measured values of the CRMs. Precision is determined by repeat analysis of samples and CRMs. Spectrophotometric pH measurements require no calibration. Precision of pH analysis is determined by repeat measurements of replicate samples. Nutrient analyses were performed by University of Tampa (R. Masserini). YSI meters used to collect ancillary data were calibrated daily. Missing values are denoted with an entry of -999. The discrete data were generated using certified reference materials to correct measured values and validate the quality of the data reported. Missing data were the result of either technological failure, or lack of data collection due to sampling design or inclement weather. Quality flags indicating the status of individual data points are follows; Flag 0 = No quality control, Flag 1 = Good data, Flag 2= Probably good data, Flag 3 = Probably bad data, Flag 4 = Bad data, Flag 5 = Changed by analyst due to correction, Flag 9 = The value of the element is missing. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. Accuracy was checked against global positioning system (GPS) values provided by the manufacture, Garmin GPSMAP64st and Garmin GPSmpa60CSx FPSMAP60 accuracy < 10 meters Accuracy was checked against GPS values provided by the manufacture, Garmin GPSMAP64st and Garmin GPSmpa60CSx FPSMAP60 accuracy <10 meters Detailed information on sampling procedures and analyses is provided in Yates, K.K, Moyer, R.P., Moore, C., Tomasko, D., Smiley, N., Torres-Garcia, L., Powell, C.E., Chappel, A.R., Bociu, I. 2017. Ocean acidification buffering effects of seagrass in Tampa Bay. In, Burke, M. (ed.), Proceedings: The 6th Tampa Bay Area Scientific Information Symposium, BASIS 6, pp. 273-284. http://www.tbeptech.org/data/tbep-basis/213-6th-bay-area-scientific-information-symposium-proceedings. 2015 Kimberly Yates USGS mailing and physical

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St. Petersburg Florida 33701 USA 727-502-8059 kyates@usgs.gov Added keywords section with USGS persistent identifier as theme keyword. 20201013 U.S. Geological Survey VeeAnn A. Cross Marine Geologist Mailing and Physical

384 Woods Hole Road

Woods Hole MA 02543-1598 508-548-8700 x2251 508-457-2310 vatnipp@usgs.gov Point Tampa-Bay-Discrete-Data Time series measurements from discrete samples along spatial transects in Tampa Bay, FL, in May 2015 and January 2016. Spreadsheet contains: site description, latitude, longitude, datetag, timetag, water temperature, salinity, pH, dissolved oxygen, total alkalinity, dissolved inorganic carbon, nutrients, weather, tide, wind speed, wind direction, other notes USGS Site Description Description of the physical location and attributes of the location where the measurements were collected. USGS The name of the geographic location and a brief description of the seafloor characteristics at the sampling location. Latitude Latitude coordinates in decimal degrees, measured in the World Geodetic System of 1984. USGS 27.63571 27.96152 Decimal degrees Longitude Longitude coordinates in decimal degrees, measured in the World Geodetic System of 1984. USGS -82.6937 -82.55393 DATETAG A numeric identifier for calendar date, showing when each sample was collected. USGS 5/13/15 1/21/16 Units correspond to month, day and year in the format MM/DD/YY TIMETAG Timestamp showing the time of day each sample was collected. USGS 00:00:00 23:59:59 Hours, minutes, seconds in the format HH:MM:SS T(W) (C) Water temperature, measured in degrees Celsius. USGS 13.4 39.4 QF_T(W) A numeric value that indicates the quality of the reported temperature data. NOAA National Centers for Environmental Information (NCEI) https://www.nodc.noaa.gov/GTSPP/document/codetbls/gtsppcodes/gtspp_qual.html 1 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI SALINITY A numeric value for salinity. USGS 22.25 34.15 QF_SALINITY A numeric value that indicates the quality of the reported salinity data. NOAA NCEI 1, 3 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI pHT A numeric value for pH using the total scale. Missing data points are marked in data columns with the value -999. USGS 7.641 8.625 QF_pHT A numeric value that indicates the quality of pHT data reported. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI OXYGEN (mg/L) A numeric identifier for the concentration of dissolved oxygen measured in a liter of seawater. Note: Dissolved oxygen data reported for May 2015 was calculated using an Excel Macro provided by an approved YSI distributor that was then cross validated using USGS software. USGS 1.52 14.308 mg/L QF_OXYGEN A numeric value that indicates the quality of the reported oxygen data. NOAA NCEI 1, 5 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI TA (micromol/kg) A numeric identifier for the total alkalinity measured in micromoles per kilogram of seawater. Missing data points are marked in data columns with the value -999. USGS 2234.7 2575.9 micromol/kg QF_TA A numeric value that indicates the quality of the reported total alkalinity data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI DIC (micromol/kg) A numeric identifier for dissolved inorganic carbon measured in micromoles per kilogram of seawater. USGS 1821.1 2435.4 micromol/kg QF_DIC A numeric value that indicates the quality of the reported dissolved inorganic carbon data. NOAA NCEI 1 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI [NO3 + NO2] (micromol/L) A numeric identifier for the concentration of nitrate + nitrite measured in micromoles per liter of seawater. Missing data points are marked in the data columns with the value -999. USGS 0.0 0.745 micromol/L QF_[NO3+NO2] A numeric value that indicates the quality of the reported nitrate + nitrite data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI [NO2] (micromol/L) A numeric identifier for nitrite concentration measured in micromoles per liter of seawater. Missing data points are marked in data columns with the value -999. USGS 0.0 0.092 micromol/L QF_[NO2] A numeric value that indicates the quality of the reported nitrite data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI [SIL] (micromol/L) A numeric identifier for silicate concentration measured in micromoles per liter of seawater. Missing data points are marked in data columns with the value -999. USGS 0.0 18.177 micromol/L QF_[SIL] A numeric value that indicates the quality of the reported silicate data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI [NH4] (micromol/L) A numeric identifier for ammonium concentration measured in micromoles per liter of seawater. Missing data points are marked in data columns with the value -999. USGS 0.0 4.61 micromol/L QF_[NH4] A numeric value that indicates the quality of reported ammonium data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI [PO43-] (micromol/L) A numeric value for phosphate concentration measured in micromoles per liter of seawater. Missing data points are marked in data columns with the value -999. USGS 0.142 3.242 micromol/L QF_[PO43-) A numeric value that indicates the quality of the reported phosphate data. NOAA NCEI 1, 9 Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. NOAA NCEI Weather The weather conditions at the location where the sample was taken. Missing data points are marked in data columns with the value -999. USGS This field describes the general weather conditions at the location where the data were collected. Tide (m) A numeric value representing tide levels when sample was collected, measured in meters relative to mean lower low water. Missing data points are marked in data columns with -999. NOAA https://tidesandcurrents.noaa.gov/ -0.22 0.76 m Wind (m/s) A numeric value for the speed of the wind at the time of sample collection, measured in meters per second. USGS 0 7.4 m/s Wind Direction A numeric value for the heading at which the wind was moving. Missing data points are marked in data columns with the value -999. USGS 0 344 degrees Other Notes This field contains additional observed environmental conditions at time of sample collection. USGS This field contains additional notes about the conditions of the environment that may influence sample results. Kimberly Yates United States Geological Survey mailing and physical

600 4th Street South

St. Petersburg Florida 33701 USA 727-502-8059 kyates@usgs.gov Resource_Desciption: Tampa-Bay-Discrete-Data-May-2015.csv, Tampa-Bay-Discrete-Data-Jan-2016.csv Although these data have been processed successfully on a computer system at the USGS, no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described and (or) contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. CSV (Comma Separated Values) none https://coastal.er.usgs.gov/data-release/doi-F7Q23XSF/data/Tampa-Bay-Discrete-Data-May-2015.zip https://coastal.er.usgs.gov/data-release/doi-F7Q23XSF/data/Tampa-Bay-Discrete-Data-Jan-2016.zip None 20201013 Kimberly Yates United States Geological Survey Research Oceanographer mailing and physical

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St. Petersburg FL 33701 USA 727-502-8059 kyates@usgs.gov Content Standard for Digital Geospatial Metadata FGDC-STD-001-1998