John T. Lisle
20210226
Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: Uptake and Inactivation Data
2.0
Tabular digital data
U.S. Geological Survey Data Release
doi:10.5066/P9EOM5RC
St. Petersburg, FL
U.S. Geological Survey
https://doi.org/10.5066/P9EOM5RC
This metadata record describes the microbial uptake of nutrients by native planktonic and biofilm-associated bacteria and the inactivation of E. coli, MS2 bacteriophage, polio virus and Cryptosporidium parvum in the Upper Floridan Aquifer (approximately 1,000 feet below land surface) in the Okeechobee, Florida area. Groundwater samples were collected or accessed via the use of a mesocosm between 2018 and 2020 from a South Florida Water Management District (SFWMD) monitoring well installed at the Kissimmee River Aquifer Storage and Recovery (ASR) facility, which is located at the discharge point of the Kissimmee River into Lake Okeechobee.
Data were collected to quantify the nutrient uptake and microbial inactivation rates under native groundwater geochemical conditions.
20180101
20201106
ground condition
As needed
-80.9280
-80.8072
27.2009
27.0885
USGS Metadata Identifier
USGS:2684e9d0-bc26-4933-b219-327e9cb38eea
USGS Thesaurus
biogeochemical cycling
nutrient content (water)
Marine Realms Information Bank (MRIB) keywords
nutrient cycle
groundwater
groundwater geochemistry
Common geographic areas
Southern Florida
None
Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey (USGS) requests to be acknowledged as originator of these data in future products or derivative research.
John T Lisle
Southeast Region: ST. PETE COASTAL & MARINE SC
Research Microbiologist
mailing and physical
600 4Th Street South
St. Petersburg
FL
33701
United States
727-502-8140
jlisle@usgs.gov
USGS and South Florida Water Management District.
No formal attribute accuracy tests were conducted.
No formal logical accuracy tests were conducted.
Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
No formal positional accuracy tests were conducted.
No formal positional accuracy tests were conducted.
Between 2018 and 2020 groundwater samples were collected from a SFWMD monitoring well located at a study site near Lake Okeechobee, Florida to conduct two types of experiments. The first was a nutrient uptake experiment where a standardized solution of nitrogen (KNO3), phosphorus (K2PO4) and carbon (Na-acetate) was added to each of the respective experimental solutions, then sampled over a period of time to quantify the rates at which the native planktonic and biofilm-associated bacteria removed these nutrients and either incorporated them into bacterial biomass and/or carbon dioxide. The data from the nitrogen removal experiments were generated on a Seal Analytical Auto Analyzer 3, while the data from the phosphorus and carbon uptake experiments were generated on a Perkin Elmer TriCarb liquid scintillation counter following the measurement of samples containing 32P (as phosphate) and 14C (as acetate).
20190801
Between January and October 2020 known concentrations of E. coli, bacteriophage MS2, human enterovirus polio, and Cryptosporidium parvum were added to filtered sterilized groundwater from the same SFWMD monitoring well used for the nutrient uptake experiments and described in the previous step. These solutions of microorganisms were then transferred to diffusion chambers that were suspended in a constant flow of groundwater within a mesocosm at the wellhead. Periodically, a diffusion chamber for each of the microorganisms was recovered from the mesocosm and processed for the recovery of each type of microorganism using methods specific to the recovery of that microorganism.
20201106
Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.csv, Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.xlsx
These files include data associated with the analysis of microbial nutrient uptake and physiological inactivation observed in samples collected from the Upper Floridan Aquifer.
USGS
Geochemical parameter
Groundwater geochemical parameters measured at the well head.
USGS
Temperature, pH, Oxidation Reduction Potential, Specific conductance, Turbidity, Color, Total dissolved solids, Total Alkalinity (as CaCO3), Aluminum, Barium, Boron, Bromide, Calcium, Chloride, Copper, Fluoride, Iron, Magnesium, Manganese, Potassium, Silica, Sodium, Sulfate, Sulfide, Zinc, NO2-N, NO3-N, NH3-N, Total PO4-P, Ortho PO4-P, Total organic carbon, and Dissolved organic carbon.
Geochemical parameter units
Geochemical parameter units
USGS
degrees Celsius
Temperature units
USGS
not applicable
There are no units for pH
USGS
millivolts
Oxidation reduction potential units
USGS
microsiemens/cm
Specific conductance units
USGS
nephelometric turbidity units (NTU)
Turbidity units
USGS
platinum cobalt units (PCU)
Color units
USGS
mg/L
Concentration units as milligrams per liter
USGS
Geochemical parameter mean and standard deviation
Arithmetic mean with 1 standard deviation value for each of the geochemical parameters.
USGS
The actual measured values for each of the respective geochemical parameters.
Time (hrs) Nitrogen Planktonic
Experimental sample collection times, in hours, for the nitrogen removal experiment.
USGS
0.0
169.75
NO3-N (mg/L) Planktonic
The concentrations of nitrate-nitrogen observed in samples.
USGS
0.0414
0.4231
NO2-N (mg/L) Planktonic
The concentrations of nitrite-nitrogen observed in samples.
USGS
0.0003
0.3678
NH4-N (mg/L) Planktonic
The concentrations of ammonia-nitrogen observed in samples.
USGS
0.4267
0.4342
Time (hrs) Nitrogen Biofilm
Experimental sample collection times, in hours, for the nitrogen removal experiment.
USGS
0.0
169.25
NO3-N (mg/L) Biofilm
The concentrations of nitrate-nitrogen observed in samples.
USGS
0.0042
0.3938
NO2-N (mg/L) Biofilm
The concentrations of nitrite-nitrogen observed in samples.
USGS
0.0006
0.1241
NH4-N (mg/L) Biofilm
The concentrations of ammonia-nitrogen observed in samples.
USGS
0.4186
0.4489
Time (hrs) Phosphorus Planktonic
Experimental sample collection times, in hours, for the phosphorus uptake experiment.
USGS
0.0
6.0
PO4-P (mg/L) Planktonic
The concentrations of phosphorus observed in samples.
USGS
0.0
0.0032
Time (hrs) Phosphorus Biofilm
Experimental sample collection times, in hours, for the phosphorus uptake experiment.
USGS
0.0
6.0
PO4-P (mg/L) Biofilm
The concentrations of phosphorus observed in samples.
USGS
0.0
0.477
Time (hrs) Carbon Planktonic
Experimental sample collection times, in hours, for the carbon uptake experiment.
USGS
0.0
123.0
CO2 (mg/L) Planktonic
The concentrations of carbon dioxide observed in samples.
USGS
0.0
0.24479
Biomass (mg/L) Planktonic
The concentrations of carbon observed in the bacterial biomass samples.
USGS
0.0
0.40887
Time (hrs) Carbon Biofilm
Experimental sample collection times, in hours, for the carbon uptake experiment.
USGS
0.0
122.0
CO2 (mg/L) Biofilm
The concentrations of carbon dioxide observed in samples.
USGS
0.0
0.38774
Biomass (mg/L) Biofilm
The concentrations of carbon observed in the bacterial biomass samples.
USGS
0.0
1.9413
Time (hrs) MS2_1
Experimental sample collection times, in hours, for the MS2 bacteriophage in the first inactivation experiment.
USGS
0.00
504.00
MS2_1 (PFU/mL)
The number of plaque forming units (PFU) of MS2 in inactivation samples.
USGS
1
2085000001
Time (hrs) MS2_2
Experimental sample collection times, in hours, for the MS2 bacteriophage in the second inactivation experiment.
USGS
0.00
886.17
MS2_2 Mean (PFU/mL)
The mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment.
USGS
0
1585000
MS2_2 Standard Deviation (PFU/mL)
The standard deviation on the mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment.
USGS
0
137961
Time (hrs) Polio
Experimental sample collection times, in hours, for the polio enterovirus inactivation experiment.
USGS
0.00
1390.17
Polio (MPN/mL)
The median most probable number (MPN) of polio virus in each sample.
USGS
35
3500000
Polio MPN Upper 95% Confidence Interval
The upper 95% confidence interval for the median MPN of polio virus.
USGS
100
10000000
Polio MPN Lower 95% Confidence Interval
The lower 95% confidence interval for the median MPN of polio virus
USGS
12
1200000
Time (hrs) Cryptosporidium parvum
Experimental sample collection times, in hours, for the Cryptosporidium parvum inactivation experiment.
USGS
0.00
1390.17
Cryptosporidium parvum (MPN/mL)
The median most probable number (MPN) of Cryptosporidium parvum in each sample.
USGS
1.00
28000
Cryptosporidium parvum MPN Upper 95% Confidence Interval
The upper 95% confidence interval for the median MPN of Cryptosporidium parvum.
USGS
7.20
110000
Cryptosporidium parvum MPN Lower 95% Confidence Interval
The lower 95% confidence interval for the median MPN of Cryptosporidium parvum.
USGS
0.14
7300
John T Lisle
Southeast Region: ST. PETE COASTAL & MARINE SC
Research Microbiologist
mailing and physical
600 4Th Street South
St. Petersburg
FL
33701
United States
727-502-8140
jlisle@usgs.gov
Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
CSV, XLXS
https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.zip
https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.zip
None
20201207
John T Lisle
Southeast Region: ST. PETE COASTAL & MARINE SC
Research Microbiologist
mailing and physical
600 4Th Street South
St. Petersburg
FL
33701
United States
727-502-8140
jlisle@usgs.gov
Content Standard for Digital Geospatial Metadata
FGDC-STD-001-1998