Metadata: Identification_Information: Citation: Citation_Information: Originator: John T. Lisle Publication_Date: 20210226 Title: Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: Uptake and Inactivation Data Edition: 2.0 Geospatial_Data_Presentation_Form: Tabular digital data Series_Information: Series_Name: U.S. Geological Survey Data Release Issue_Identification: doi:10.5066/P9EOM5RC Publication_Information: Publication_Place: St. Petersburg, FL Publisher: U.S. Geological Survey Online_Linkage: https://doi.org/10.5066/P9EOM5RC Description: Abstract: This metadata record describes the microbial uptake of nutrients by native planktonic and biofilm-associated bacteria and the inactivation of E. coli, MS2 bacteriophage, polio virus and Cryptosporidium parvum in the Upper Floridan Aquifer (approximately 1,000 feet below land surface) in the Okeechobee, Florida area. Groundwater samples were collected or accessed via the use of a mesocosm between 2018 and 2020 from a South Florida Water Management District (SFWMD) monitoring well installed at the Kissimmee River Aquifer Storage and Recovery (ASR) facility, which is located at the discharge point of the Kissimmee River into Lake Okeechobee. Purpose: Data were collected to quantify the nutrient uptake and microbial inactivation rates under native groundwater geochemical conditions. Time_Period_of_Content: Time_Period_Information: Range_of_Dates/Times: Beginning_Date: 20180101 Ending_Date: 20201106 Currentness_Reference: ground condition Status: Progress: In work Maintenance_and_Update_Frequency: As needed Spatial_Domain: Bounding_Coordinates: West_Bounding_Coordinate: -80.9280 East_Bounding_Coordinate: -80.8072 North_Bounding_Coordinate: 27.2009 South_Bounding_Coordinate: 27.0885 Keywords: Theme: Theme_Keyword_Thesaurus: USGS Metadata Identifier Theme_Keyword: USGS:2684e9d0-bc26-4933-b219-327e9cb38eea Theme: Theme_Keyword_Thesaurus: USGS Thesaurus Theme_Keyword: biogeochemical cycling Theme_Keyword: nutrient content (water) Theme: Theme_Keyword_Thesaurus: Marine Realms Information Bank (MRIB) keywords Theme_Keyword: nutrient cycle Theme_Keyword: groundwater Theme_Keyword: groundwater geochemistry Place: Place_Keyword_Thesaurus: Common geographic areas Place_Keyword: Southern Florida Access_Constraints: None Use_Constraints: Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey (USGS) requests to be acknowledged as originator of these data in future products or derivative research. Point_of_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: John T Lisle Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC Contact_Position: Research Microbiologist Contact_Address: Address_Type: mailing and physical Address: 600 4Th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8140 Contact_Electronic_Mail_Address: jlisle@usgs.gov Data_Set_Credit: USGS and South Florida Water Management District. Data_Quality_Information: Attribute_Accuracy: Attribute_Accuracy_Report: No formal attribute accuracy tests were conducted. Logical_Consistency_Report: No formal logical accuracy tests were conducted. Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. Positional_Accuracy: Horizontal_Positional_Accuracy: Horizontal_Positional_Accuracy_Report: No formal positional accuracy tests were conducted. Vertical_Positional_Accuracy: Vertical_Positional_Accuracy_Report: No formal positional accuracy tests were conducted. Lineage: Process_Step: Process_Description: Between 2018 and 2020 groundwater samples were collected from a SFWMD monitoring well located at a study site near Lake Okeechobee, Florida to conduct two types of experiments. The first was a nutrient uptake experiment where a standardized solution of nitrogen (KNO3), phosphorus (K2PO4) and carbon (Na-acetate) was added to each of the respective experimental solutions, then sampled over a period of time to quantify the rates at which the native planktonic and biofilm-associated bacteria removed these nutrients and either incorporated them into bacterial biomass and/or carbon dioxide. The data from the nitrogen removal experiments were generated on a Seal Analytical Auto Analyzer 3, while the data from the phosphorus and carbon uptake experiments were generated on a Perkin Elmer TriCarb liquid scintillation counter following the measurement of samples containing 32P (as phosphate) and 14C (as acetate). Process_Date: 20190801 Process_Step: Process_Description: Between January and October 2020 known concentrations of E. coli, bacteriophage MS2, human enterovirus polio, and Cryptosporidium parvum were added to filtered sterilized groundwater from the same SFWMD monitoring well used for the nutrient uptake experiments and described in the previous step. These solutions of microorganisms were then transferred to diffusion chambers that were suspended in a constant flow of groundwater within a mesocosm at the wellhead. Periodically, a diffusion chamber for each of the microorganisms was recovered from the mesocosm and processed for the recovery of each type of microorganism using methods specific to the recovery of that microorganism. Process_Date: 20201106 Entity_and_Attribute_Information: Detailed_Description: Entity_Type: Entity_Type_Label: Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.csv, Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.xlsx Entity_Type_Definition: These files include data associated with the analysis of microbial nutrient uptake and physiological inactivation observed in samples collected from the Upper Floridan Aquifer. Entity_Type_Definition_Source: USGS Attribute: Attribute_Label: Geochemical parameter Attribute_Definition: Groundwater geochemical parameters measured at the well head. Attribute_Definition_Source: USGS Attribute_Domain_Values: Unrepresentable_Domain: Temperature, pH, Oxidation Reduction Potential, Specific conductance, Turbidity, Color, Total dissolved solids, Total Alkalinity (as CaCO3), Aluminum, Barium, Boron, Bromide, Calcium, Chloride, Copper, Fluoride, Iron, Magnesium, Manganese, Potassium, Silica, Sodium, Sulfate, Sulfide, Zinc, NO2-N, NO3-N, NH3-N, Total PO4-P, Ortho PO4-P, Total organic carbon, and Dissolved organic carbon. Attribute: Attribute_Label: Geochemical parameter units Attribute_Definition: Geochemical parameter units Attribute_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: degrees Celsius Enumerated_Domain_Value_Definition: Temperature units Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: not applicable Enumerated_Domain_Value_Definition: There are no units for pH Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: millivolts Enumerated_Domain_Value_Definition: Oxidation reduction potential units Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: microsiemens/cm Enumerated_Domain_Value_Definition: Specific conductance units Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: nephelometric turbidity units (NTU) Enumerated_Domain_Value_Definition: Turbidity units Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: platinum cobalt units (PCU) Enumerated_Domain_Value_Definition: Color units Enumerated_Domain_Value_Definition_Source: USGS Attribute_Domain_Values: Enumerated_Domain: Enumerated_Domain_Value: mg/L Enumerated_Domain_Value_Definition: Concentration units as milligrams per liter Enumerated_Domain_Value_Definition_Source: USGS Attribute: Attribute_Label: Geochemical parameter mean and standard deviation Attribute_Definition: Arithmetic mean with 1 standard deviation value for each of the geochemical parameters. Attribute_Definition_Source: USGS Attribute_Domain_Values: Unrepresentable_Domain: The actual measured values for each of the respective geochemical parameters. Attribute: Attribute_Label: Time (hrs) Nitrogen Planktonic Attribute_Definition: Experimental sample collection times, in hours, for the nitrogen removal experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 169.75 Attribute: Attribute_Label: NO3-N (mg/L) Planktonic Attribute_Definition: The concentrations of nitrate-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0414 Range_Domain_Maximum: 0.4231 Attribute: Attribute_Label: NO2-N (mg/L) Planktonic Attribute_Definition: The concentrations of nitrite-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0003 Range_Domain_Maximum: 0.3678 Attribute: Attribute_Label: NH4-N (mg/L) Planktonic Attribute_Definition: The concentrations of ammonia-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.4267 Range_Domain_Maximum: 0.4342 Attribute: Attribute_Label: Time (hrs) Nitrogen Biofilm Attribute_Definition: Experimental sample collection times, in hours, for the nitrogen removal experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 169.25 Attribute: Attribute_Label: NO3-N (mg/L) Biofilm Attribute_Definition: The concentrations of nitrate-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0042 Range_Domain_Maximum: 0.3938 Attribute: Attribute_Label: NO2-N (mg/L) Biofilm Attribute_Definition: The concentrations of nitrite-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0006 Range_Domain_Maximum: 0.1241 Attribute: Attribute_Label: NH4-N (mg/L) Biofilm Attribute_Definition: The concentrations of ammonia-nitrogen observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.4186 Range_Domain_Maximum: 0.4489 Attribute: Attribute_Label: Time (hrs) Phosphorus Planktonic Attribute_Definition: Experimental sample collection times, in hours, for the phosphorus uptake experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 6.0 Attribute: Attribute_Label: PO4-P (mg/L) Planktonic Attribute_Definition: The concentrations of phosphorus observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 0.0032 Attribute: Attribute_Label: Time (hrs) Phosphorus Biofilm Attribute_Definition: Experimental sample collection times, in hours, for the phosphorus uptake experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 6.0 Attribute: Attribute_Label: PO4-P (mg/L) Biofilm Attribute_Definition: The concentrations of phosphorus observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 0.477 Attribute: Attribute_Label: Time (hrs) Carbon Planktonic Attribute_Definition: Experimental sample collection times, in hours, for the carbon uptake experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 123.0 Attribute: Attribute_Label: CO2 (mg/L) Planktonic Attribute_Definition: The concentrations of carbon dioxide observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 0.24479 Attribute: Attribute_Label: Biomass (mg/L) Planktonic Attribute_Definition: The concentrations of carbon observed in the bacterial biomass samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 0.40887 Attribute: Attribute_Label: Time (hrs) Carbon Biofilm Attribute_Definition: Experimental sample collection times, in hours, for the carbon uptake experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 122.0 Attribute: Attribute_Label: CO2 (mg/L) Biofilm Attribute_Definition: The concentrations of carbon dioxide observed in samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 0.38774 Attribute: Attribute_Label: Biomass (mg/L) Biofilm Attribute_Definition: The concentrations of carbon observed in the bacterial biomass samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.0 Range_Domain_Maximum: 1.9413 Attribute: Attribute_Label: Time (hrs) MS2_1 Attribute_Definition: Experimental sample collection times, in hours, for the MS2 bacteriophage in the first inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.00 Range_Domain_Maximum: 504.00 Attribute: Attribute_Label: MS2_1 (PFU/mL) Attribute_Definition: The number of plaque forming units (PFU) of MS2 in inactivation samples. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 1 Range_Domain_Maximum: 2085000001 Attribute: Attribute_Label: Time (hrs) MS2_2 Attribute_Definition: Experimental sample collection times, in hours, for the MS2 bacteriophage in the second inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.00 Range_Domain_Maximum: 886.17 Attribute: Attribute_Label: MS2_2 Mean (PFU/mL) Attribute_Definition: The mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0 Range_Domain_Maximum: 1585000 Attribute: Attribute_Label: MS2_2 Standard Deviation (PFU/mL) Attribute_Definition: The standard deviation on the mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0 Range_Domain_Maximum: 137961 Attribute: Attribute_Label: Time (hrs) Polio Attribute_Definition: Experimental sample collection times, in hours, for the polio enterovirus inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.00 Range_Domain_Maximum: 1390.17 Attribute: Attribute_Label: Polio (MPN/mL) Attribute_Definition: The median most probable number (MPN) of polio virus in each sample. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 35 Range_Domain_Maximum: 3500000 Attribute: Attribute_Label: Polio MPN Upper 95% Confidence Interval Attribute_Definition: The upper 95% confidence interval for the median MPN of polio virus. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 100 Range_Domain_Maximum: 10000000 Attribute: Attribute_Label: Polio MPN Lower 95% Confidence Interval Attribute_Definition: The lower 95% confidence interval for the median MPN of polio virus Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 12 Range_Domain_Maximum: 1200000 Attribute: Attribute_Label: Time (hrs) Cryptosporidium parvum Attribute_Definition: Experimental sample collection times, in hours, for the Cryptosporidium parvum inactivation experiment. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.00 Range_Domain_Maximum: 1390.17 Attribute: Attribute_Label: Cryptosporidium parvum (MPN/mL) Attribute_Definition: The median most probable number (MPN) of Cryptosporidium parvum in each sample. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 1.00 Range_Domain_Maximum: 28000 Attribute: Attribute_Label: Cryptosporidium parvum MPN Upper 95% Confidence Interval Attribute_Definition: The upper 95% confidence interval for the median MPN of Cryptosporidium parvum. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 7.20 Range_Domain_Maximum: 110000 Attribute: Attribute_Label: Cryptosporidium parvum MPN Lower 95% Confidence Interval Attribute_Definition: The lower 95% confidence interval for the median MPN of Cryptosporidium parvum. Attribute_Definition_Source: USGS Attribute_Domain_Values: Range_Domain: Range_Domain_Minimum: 0.14 Range_Domain_Maximum: 7300 Distribution_Information: Distributor: Contact_Information: Contact_Person_Primary: Contact_Person: John T Lisle Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC Contact_Position: Research Microbiologist Contact_Address: Address_Type: mailing and physical Address: 600 4Th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8140 Contact_Electronic_Mail_Address: jlisle@usgs.gov Distribution_Liability: Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Standard_Order_Process: Digital_Form: Digital_Transfer_Information: Format_Name: CSV, XLXS Digital_Transfer_Option: Online_Option: Computer_Contact_Information: Network_Address: Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.zip Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.zip Fees: None Metadata_Reference_Information: Metadata_Date: 20201207 Metadata_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: John T Lisle Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC Contact_Position: Research Microbiologist Contact_Address: Address_Type: mailing and physical Address: 600 4Th Street South City: St. Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8140 Contact_Electronic_Mail_Address: jlisle@usgs.gov Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata Metadata_Standard_Version: FGDC-STD-001-1998