Metadata:
  Identification_Information:
    Citation:
      Citation_Information:
        Originator: John T. Lisle
        Publication_Date: 20200720
        Title: Microbial Ecology of the Floridan Aquifer near Okeechobee, FL
        Geospatial_Data_Presentation_Form: Tabular digital data
        Series_Information:
          Series_Name: U.S. Geological Survey Data Release
          Issue_Identification: doi:10.5066/P9EOM5RC
        Publication_Information:
          Publication_Place: St. Petersburg, FL
          Publisher: U.S. Geological Survey
        Online_Linkage: https://doi.org/10.5066/P9EOM5RC
        Larger_Work_Citation:
          Citation_Information:
            Originator: John T. Lisle
            Publication_Date: 2020
            Title: Nutrient Removal and Uptake by Native Planktonic and Biofilm Bacterial Communities in An Anaerobic Aquifer
            Geospatial_Data_Presentation_Form: Publication
            Publication_Information:
              Publication_Place: Lausanne, Switzerland
              Publisher: Frontiers in Microbiology
            Online_Linkage: https://doi.org/10.3389/fmicb.2020.01765
    Description:
      Abstract: This metadata record describes the microbial uptake of nutrients by native planktonic and biofilm-associated bacteria within the Upper Floridan Aquifer (approximately 1,000 feet below land surface) in the Okeechobee, Florida area. Groundwater samples were collected between 2018 and 2019 from a South Florida Water Management District (SFWMD) monitoring well installed at the Kissimmee River Aquifer Storage and Recovery (ASR) facility, which is located at the discharge point of the Kissimmee River into Lake Okeechobee.
      Purpose: Data were collected to quantify the nutrient uptake rates under native groundwater geochemical conditions.
    Time_Period_of_Content:
      Time_Period_Information:
        Range_of_Dates/Times:
          Beginning_Date: 20180101
          Ending_Date: 20191001
      Currentness_Reference: ground condition
    Status:
      Progress: In work
      Maintenance_and_Update_Frequency: As needed
    Spatial_Domain:
      Bounding_Coordinates:
        West_Bounding_Coordinate: -80.9280
        East_Bounding_Coordinate: -80.8072
        North_Bounding_Coordinate: 27.2009
        South_Bounding_Coordinate: 27.0885
    Keywords:
      Theme:
        Theme_Keyword_Thesaurus: USGS Metadata Identifier
        Theme_Keyword: USGS:109553b0-9536-4bd3-a2af-f019fc7a3bc4
      Theme:
        Theme_Keyword_Thesaurus: ISO 19115 Topic Category
        Theme_Keyword: environment
        Theme_Keyword: biota
        Theme_Keyword: health
      Theme:
        Theme_Keyword_Thesaurus: USGS Thesaurus
        Theme_Keyword: biogeochemical cycling
        Theme_Keyword: nutrient content (water)
      Theme:
        Theme_Keyword_Thesaurus: Marine Realms Information Bank (MRIB) keywords
        Theme_Keyword: nutrient cycle
        Theme_Keyword: groundwater
        Theme_Keyword: groundwater geochemistry
      Place:
        Place_Keyword_Thesaurus: Common geographic areas
        Place_Keyword: Southern Florida
    Access_Constraints: None
    Use_Constraints: None
    Point_of_Contact:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: John T Lisle
          Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC
        Contact_Position: Research Microbiologist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: FL
          Postal_Code: 33701
          Country: United States
        Contact_Voice_Telephone: 727-502-8140
        Contact_Electronic_Mail_Address: jlisle@usgs.gov
    Data_Set_Credit: USGS and South Florida Water Management District.
  Data_Quality_Information:
    Attribute_Accuracy:
      Attribute_Accuracy_Report: No formal attribute accuracy tests were conducted.
    Logical_Consistency_Report: No formal logical accuracy tests were conducted.
    Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
    Positional_Accuracy:
      Horizontal_Positional_Accuracy:
        Horizontal_Positional_Accuracy_Report: No formal positional accuracy tests were conducted.
      Vertical_Positional_Accuracy:
        Vertical_Positional_Accuracy_Report: No formal positional accuracy tests were conducted.
    Lineage:
      Process_Step:
        Process_Description: Between 2018 and 2019 groundwater samples were collected from a SFWMD monitoring well located at a study site near Lake Okeechobee. Samples were dosed with standardized solutions of nitrogen (KNO3), phosphorus (K2PO4) and carbon (Na-acetate) then sampled over a period of time to quantify the rates at which the native planktonic and biofilm-associated bacteria removed these nutrients and either incorporated them into bacterial biomass and/or carbon dioxide. The data from the nitrogen removal experiments were generated on a Seal Analytical Auto Analyzer 3, while the data from the phosphorus and carbon uptake experiments were generated on a Perkin Elmer TriCarb liquid scintillation counter following the measurement of samples containing 32P (as phosphate) and 14C (as acetate).
        Process_Date: 20190801
      Process_Step:
        Process_Description: Added keywords section with USGS persistent identifier as theme keyword.
        Process_Date: 20201013
        Process_Contact:
          Contact_Information:
            Contact_Organization_Primary:
              Contact_Organization: U.S. Geological Survey
              Contact_Person: VeeAnn A. Cross
            Contact_Position: Marine Geologist
            Contact_Address:
              Address_Type: Mailing and Physical
              Address: 384 Woods Hole Road
              City: Woods Hole
              State_or_Province: MA
              Postal_Code: 02543-1598
            Contact_Voice_Telephone: 508-548-8700 x2251
            Contact_Facsimile_Telephone: 508-457-2310
            Contact_Electronic_Mail_Address: vatnipp@usgs.gov
  Entity_and_Attribute_Information:
    Detailed_Description:
      Entity_Type:
        Entity_Type_Label: Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.csv, Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.xlsx
        Entity_Type_Definition: These files include data associated with the analysis of microbial nutrient uptake observed in samples collected from the Upper Floridan Aquifer.
        Entity_Type_Definition_Source: USGS
      Attribute:
        Attribute_Label: Geochemical parameter
        Attribute_Definition: Groundwater geochemical parameters measured at the well head.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Unrepresentable_Domain: Temperature, pH, Oxidation Reduction Potential, Specific conductance, Turbidity, Color, Total dissolved solids, Total Alkalinity (as  CaCO3), Aluminum, Barium, Boron, Bromide, Calcium, Chloride, Copper, Fluoride, Iron, Magnesium, Manganese, Potassium, Silica, Sodium, Sulfate, Sulfide, Zinc, NO2-N, NO3-N, NH3-N, Total PO4-P, Ortho PO4-P, Total organic carbon, Dissolved organic carbon
      Attribute:
        Attribute_Label: Geochemical parameter units
        Attribute_Definition: Geochemical parameter units
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: degrees Celsius
            Enumerated_Domain_Value_Definition: Temperature units
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: not applicable
            Enumerated_Domain_Value_Definition: There are no units for pH
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: millivolts
            Enumerated_Domain_Value_Definition: Oxidation reduction potential units
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: microsiemens/cm
            Enumerated_Domain_Value_Definition: Specific conductance units
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: nephelometric turbidity units (NTU)
            Enumerated_Domain_Value_Definition: Turbidity units
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: platinum cobalt units (PCU)
            Enumerated_Domain_Value_Definition: Color units
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: mg/L
            Enumerated_Domain_Value_Definition: Concentration units as milligrams per liter
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Geochemical parameter mean and standard deviation
        Attribute_Definition: Arithmetic mean with 1 standard deviation value for each of the geochemical parameters.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Unrepresentable_Domain: The actual measured values for each of the respective geochemical parameters.
      Attribute:
        Attribute_Label: Time (hrs) Nitrogen Planktonic
        Attribute_Definition: Experimental sample collection times, in hours, for the nitrogen removal experiment.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 169.75
      Attribute:
        Attribute_Label: NO3-N (mg/L) Planktonic
        Attribute_Definition: The concentrations of nitrate-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0414
            Range_Domain_Maximum: 0.4231
      Attribute:
        Attribute_Label: NO2-N (mg/L) Planktonic
        Attribute_Definition: The concentrations of nitrite-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0003
            Range_Domain_Maximum: 0.3678
      Attribute:
        Attribute_Label: NH4-N (mg/L) Planktonic
        Attribute_Definition: The concentrations of ammonia-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.4267
            Range_Domain_Maximum: 0.4342
      Attribute:
        Attribute_Label: Time (hrs) Nitrogen Biofilm
        Attribute_Definition: Experimental sample collection times, in hours, for the nitrogen removal experiment.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 169.25
      Attribute:
        Attribute_Label: NO3-N (mg/L) Biofilm
        Attribute_Definition: The concentrations of nitrate-nitrogen observed in samples collected at the listed time points in Figure 2AB (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0042
            Range_Domain_Maximum: 0.3938
      Attribute:
        Attribute_Label: NO2-N (mg/L) Biofilm
        Attribute_Definition: The concentrations of nitrite-nitrogen observed in samples collected at the listed time points in Figure 2B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0006
            Range_Domain_Maximum: 0.1241
      Attribute:
        Attribute_Label: NH4-N (mg/L) Biofilm
        Attribute_Definition: The concentrations of ammonia-nitrogen observed in samples collected at the listed time points in Figure 2B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.4186
            Range_Domain_Maximum: 0.4489
      Attribute:
        Attribute_Label: Time (hrs) Phosphorus Planktonic
        Attribute_Definition: Experimental sample collection times, in hours, for the phosphorus uptake experiment graphed in Figure 3A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 6.0
      Attribute:
        Attribute_Label: PO4-P (mg/L) Planktonic
        Attribute_Definition: The concentrations of phosphorus observed in samples collected at the listed time points in Figure 3A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 0.0032
      Attribute:
        Attribute_Label: Time (hrs) Phosphorus Biofilm
        Attribute_Definition: Experimental sample collection times, in hours, for the phosphorus uptake experiment graphed in Figure 3B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 6.0
      Attribute:
        Attribute_Label: PO4-P (mg/L) Biofilm
        Attribute_Definition: The concentrations of phosphorus observed in samples collected at the listed time points in Figure 3B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 0.477
      Attribute:
        Attribute_Label: Time (hrs) Carbon Planktonic
        Attribute_Definition: Experimental sample collection times, in hours, for the carbon uptake experiment graphed in Figure 4A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 123.0
      Attribute:
        Attribute_Label: CO2 (mg/L) Planktonic
        Attribute_Definition: The concentrations of carbon dioxide observed in samples collected at the listed time points in Figure 4A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 0.24479
      Attribute:
        Attribute_Label: Biomass (mg/L) Planktonic
        Attribute_Definition: The concentrations of carbon observed in the bacterial biomass samples collected at the listed time points in Figure 4A (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 0.40887
      Attribute:
        Attribute_Label: Time (hrs) Carbon Biofilm
        Attribute_Definition: Experimental sample collection times, in hours, for the carbon uptake experiment graphed in Figure 4B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 122.0
      Attribute:
        Attribute_Label: CO2 (mg/L) Biofilm
        Attribute_Definition: The concentrations of carbon dioxide observed in samples collected at the listed time points in Figure 4B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 0.38774
      Attribute:
        Attribute_Label: Biomass (mg/L) Biofilm
        Attribute_Definition: The concentrations of carbon observed in the bacterial biomass samples collected at the listed time points in Figure 4B (Lisle, 2020).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0
            Range_Domain_Maximum: 1.9413
  Distribution_Information:
    Distributor:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: John T Lisle
          Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC
        Contact_Position: Research Microbiologist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: FL
          Postal_Code: 33701
          Country: United States
        Contact_Voice_Telephone: 727-502-8140
        Contact_Electronic_Mail_Address: jlisle@usgs.gov
    Distribution_Liability: Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
    Standard_Order_Process:
      Digital_Form:
        Digital_Transfer_Information:
          Format_Name: CSV, XLXS
        Digital_Transfer_Option:
          Online_Option:
            Computer_Contact_Information:
              Network_Address:
                Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.zip
      Fees: None
  Metadata_Reference_Information:
    Metadata_Date: 20201013
    Metadata_Contact:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: John T Lisle
          Contact_Organization: Southeast Region: ST. PETE COASTAL & MARINE SC
        Contact_Position: Research Microbiologist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: FL
          Postal_Code: 33701
          Country: United States
        Contact_Voice_Telephone: 727-502-8140
        Contact_Electronic_Mail_Address: jlisle@usgs.gov
    Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata
    Metadata_Standard_Version: FGDC-STD-001-1998