Metadata: Identification_Information: Citation: Citation_Information: Originator: Caitlin E. Reynolds Publication_Date: 20250423 Title: Gulf of America non-spinose planktic foraminifera culture study from 2020-2022: LA-ICPMS Geospatial_Data_Presentation_Form: tabular digital data Larger_Work_Citation: Citation_Information: Originator: Caitlin E. Reynolds Originator: Jennifer S. Fehrenbacher Originator: Kaustubh Thirumalai Originator: Eric J. Tappa Originator: Julie N. Richey Publication_Date: 20250423 Title: Gulf of America Non-Spinose Planktic Foraminifera Culture Study From 2020-2022 Geospatial_Data_Presentation_Form: tabular digital data Series_Information: Series_Name: U.S. Geological Survey data release Issue_Identification: doi:10.5066/P147MDIE Publication_Information: Publication_Place: St. Petersburg, Florida Publisher: U.S. Geological Survey – St. Petersburg Coastal and Marine Science Center Online_Linkage: https://doi.org/10.5066/P147MDIE Description: Abstract: The methodologies and resources which were used to cultivate Globorotalia truncatulinoides and other non-spinose planktic foraminiferal species, such as Globorotalia menardii, are described in this release and in more detail in Reynolds and others (2025). Several specimens (n=69) were collected via plankton tows from the upper 200 meters (m) of the water column from the northern Gulf of America in February 2020, December 2021, and November 2022. Size measurements and growth, notes and observations, and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) data were recorded on a few selected foraminifera individuals. The foraminifera specimen survived between 8 and 76 days in a laboratory study (initially cut short by the abrupt closure of laboratory facilities due to the spread of the Coronavirus in 2019 (COVID-19 pandemic). Purpose: The growth and observation of foraminifera in laboratory culture has been an incredibly valuable tool in studying the biomineralization and partitioning of chemical constituents during foraminiferal calcification. These studies allow for the isolation of physical and chemical conditions that control the elemental and stable isotopic composition of foraminifera and are crucial to calibration and interpretation of paleoenvironmental proxies in foraminifera. Relatively few laboratory culture studies have been carried out in non-spinose species of planktic foraminifera. This study was intended to be a pilot study to test whether G. truncatulinoides and G. menardii specimens could be successfully harvested from plankton tow samples and kept alive in a laboratory at the U.S Geological Survey St. Petersburg Coastal and Marine Science Center (USGS SPCMSC). In addition to keeping the specimen alive in a lab, further testing included attempting to induce encrustation or gametogenesis under laboratory conditions. Supplemental_Information: Following SPCMSC data management protocols, the field collection activities were assigned USGS field activity numbers (FANs), 2020-302-FA, and 2022-335-FA. Additional field and data details are available for each activity by replacing the FAN (example: 2020-302-FA) in the following url: https://cmgds.marine.usgs.gov/services/activity.php?fan=2020-302-FA. Time_Period_of_Content: Time_Period_Information: Range_of_Dates/Times: Beginning_Date: 20200208 Ending_Date: 20231016 Currentness_Reference: ground condition Status: Progress: Complete Maintenance_and_Update_Frequency: None planned Spatial_Domain: Description_of_Geographic_Extent: Gulf of America Bounding_Coordinates: West_Bounding_Coordinate: -90.340433 East_Bounding_Coordinate: -89.099333 North_Bounding_Coordinate: 28.231933 South_Bounding_Coordinate: 27.533833 Keywords: Theme: Theme_Keyword_Thesaurus: USGS Metadata Identifier Theme_Keyword: USGS:cacdbc6b-7f50-41c2-88fd-46088245a738 Theme: Theme_Keyword_Thesaurus: ISO 19115 Topic Category Theme_Keyword: biota Theme: Theme_Keyword_Thesaurus: USGS Thesaurus Theme_Keyword: protists Theme_Keyword: laboratory methods Theme_Keyword: isotopic analysis Place: Place_Keyword_Thesaurus: Common geographic areas Place_Keyword: Gulf of America Place_Keyword: Louisiana Access_Constraints: No access constraints. Please see 'Distribution Information' for details. Use_Constraints: These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Point_of_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Caitlin E. Reynolds Contact_Organization: US Geological Survey Contact_Position: Geologist Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: Saint Petersburg State_or_Province: Florida Postal_Code: 33701 Country: USA Contact_Voice_Telephone: 727-502-8046 Contact_Electronic_Mail_Address: creynolds@usgs.gov Native_Data_Set_Environment: Environment as of Metadata Creation: Macintosh HD Ventura 13.6.4.; Microsoft Excel for Mac Version 16.82. Cross_Reference: Citation_Information: Originator: Caitlin E. Reynolds Originator: Jennifer S. Fehrenbacher Originator: Kaustubh Thirumalai Originator: Eric J. Tappa Originator: Julie N. Richey Publication_Date: 20250422 Title: Insights from growing Globorotalia truncatulinoides and Globorotalia menardii in the laboratory Geospatial_Data_Presentation_Form: publication Series_Information: Series_Name: Journal of Foraminiferal Research Issue_Identification: Volume 55, Issue 2 Publication_Information: Publication_Place: McLean, VA Publisher: GeoScienceWorld Other_Citation_Details: Pages 131-143 Online_Linkage: https://doi.org/10.61551/gsjfr.55.2.131 Cross_Reference: Citation_Information: Originator: Jennifer S. Fehrenbacher Originator: Ann D. Russell Originator: Catherine V. Davis Originator: Howard J. Spero Originator: Edward Chu Originator: Bärbel Hönisch Publication_Date: 20180901 Title: Ba/Ca ratios in the non-spinose planktic foraminifer Neogloboquadrina dutertrei: Evidence for an organic aggregate microhabitat Geospatial_Data_Presentation_Form: publication Series_Information: Series_Name: Geochimica et Cosmochimica Acta Issue_Identification: Volume 236 Publication_Information: Publication_Place: Online Publisher: Elsevier BV Other_Citation_Details: Pages 361-372 Online_Linkage: https://doi.org/10.1016/j.gca.2018.03.008 Cross_Reference: Citation_Information: Originator: Jennifer S. Fehrenbacher Originator: Ann D. Russell Originator: Catherine V. Davis Originator: Alexander C. Gagnon Originator: Howard J. Spero Originator: John B. Cliff Originator: Zihua Zhu Originator: Pamela Martin Publication_Date: 20170515 Title: Link between light-triggered Mg-banding and chamber formation in the planktic foraminifera Neogloboquadrina dutertrei Geospatial_Data_Presentation_Form: publication Series_Information: Series_Name: Nature Communications Issue_Identification: Volume 8, Issue 1, Article 15441 Publication_Information: Publication_Place: Online Publisher: Springer Science and Business Media LLC Online_Linkage: https://doi.org/10.1038/ncomms15441 Data_Quality_Information: Attribute_Accuracy: Attribute_Accuracy_Report: QA/QC was completed at the Oregon State University according to protocols outlined in Fehrenbacher and others (2018). Logical_Consistency_Report: All values fall within expected ranges for each species. Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. Lineage: Process_Step: Process_Description: Thirty-nine live individuals of Globorotalia truncatulinoides specimen were collected from a vertical plankton tow in the surface mixed layer (0–80 meters) of the northern Gulf of America on February 8, 2020. The plankton tow was taken at the site of our long-term sediment trap time series (27.533833 degrees North [°N], 90.340433 degrees West [°W]) during peak annual flux of G. truncatulinoides. The upper 80 m of the water column were fully mixed with an average temperature of 21.1 degrees Celsius (ºC) and salinity of 36.3 practical salinity units (PSU). The G. truncatulinoides specimens were immediately collected and identified under a dissecting microscope, picked out using small brushes, and rinsed with filtered seawater (0.45-micrometer [µm] nitrocellulose membrane filters). The foraminifera were individually added to 70 milliliter (ml) polystyrene Falcon® culture flasks containing ambient filtered seawater. The flasks were stored in the dark at ambient temperature (20 ºC). Upon returning to the laboratory at the USGS SPCMSC in St. Petersburg, Florida, the samples were stored in a VWR International Incubator (Model 1575R) set to 20 ºC. Process_Date: 20200208 Process_Step: Process_Description: Seventeen Globorotalia menardii, one Neogloboquadrina dutertrei, one Globorotalia crassaformis, and one Pulleniatina obliquiloculata live specimens were collected December 6, 2021, from a vertical plankton tow in the upper 200 m of the water column at 28.231933°N, 89.099333°W. Averages for temperature and salinity throughout the tow collection were 22.5°C and 36.5 PSU respectively. Ten additional G. menardii live specimen were collected the following year on November 30, 2022 (28.213617°N, 89.131600°W) in the same manner. The upper 200 m had an average temperature of 20.4°C and salinity of 36.4 PSU. Species were selected based on their availability in the tow material. Individual foraminifera from both collections were identified under a dissecting microscope, rinsed with filtered seawater (0.45-µm nitrocellulose membrane filters), and stored in Strontium-87 (87Sr)-labeled seawater (strontium carbonate [SrCO3], Series LH, Batch 136990, 91.26%) inside 70 ml polystyrene Falcon® culture flasks. The 87Sr label has an 87Sr/88Sr ratio four times greater than the natural isotopic abundance ratios and was chosen to geochemically observe growth in culture (Fehrenbacher and others, 2018). The flasks were stored in the dark at ambient temperature (20 ºC). Once back in the laboratory, the samples were stored in a VWR International Incubator (Model 1575R) set to 20 ºC. Process_Date: 20221130 Process_Step: Process_Description: Eighteen individual foraminifera were prepared for elemental analysis via LA-ICPMS (6 of those are included in this data release). Individual specimens were oxidatively cleaned in a solution of hydrogen peroxide (H2O2) and sodium hydroxide (NaOH) (1:1 solution 30% H2O2:0.1N NaOH) and rinsed 3 times with Milli-Q water to remove organic residue according to previously published methods for cultured foraminifera (Fehrenbacher and others, 2017). Once cleaned, each foraminifera specimen was weighed on a Mettler Toledo UMXZ Microbalance and sent to the University of Oregon for analysis. Process_Date: 2022 Process_Step: Process_Description: The University of Oregon completed the following analyses (as part of the LA-ICPMS) on the 18 foraminifera specimen: Barium/Calcium ratio (Ba/Ca), Magnesium/Calcium ratio (Mg/Ca), 88 Strontium/Calcium ratio (88Sr/Ca), 87 Strontium/Calcium (87Sr/Ca), Manganese/Calcium ratio (Mn/Ca), and 87 Strontium/88 Strontium (87Sr/88Sr). The results from these analyses for 6 specimen were compiled into Microsoft Excel (.xlsx) and comma-separated values (.csv) files and are included in this release (Foraminifera_LA-ICPMS_data.zip). Process_Date: 20231016 Source_Produced_Citation_Abbreviation: LA-ICPMS_data Process_Contact: Contact_Information: Contact_Person_Primary: Contact_Person: Jennifer Fehrenbacher Contact_Organization: Oregon State University - College of Earth, Ocean and Atmospheric Sciences Contact_Address: Address_Type: mailing and physical Address: Burt Hall 212, 2651 SW Orchard Avenue City: Corvallis State_or_Province: OR Postal_Code: 97331 Country: US Contact_Voice_Telephone: 541-737-6285 Contact_Electronic_Mail_Address: jennifer.fehrenbacher@oregonstate.edu Entity_and_Attribute_Information: Overview_Description: Entity_and_Attribute_Overview: This metadata has an associated data dictionary (Data_Dictionary_LA-ICPMS_data.docx), which describes the attributes (data columns) for the 6 foraminifera specimen included in the LA-ICPMS analyses. The metadata for the foraminifera data are not complete if they are not distributed with this document. Entity_and_Attribute_Detail_Citation: The entity and attribute information were generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information. Distribution_Information: Distributor: Contact_Information: Contact_Organization_Primary: Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center Contact_Person: USGS SPCMSC Data Management Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: Saint Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8000 Contact_Electronic_Mail_Address: gs-g-spcmsc_data_inquiries@usgs.gov Resource_Description: LA-ICPMS_data.csv, LA-ICPMS_data.xlsx Distribution_Liability: Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Standard_Order_Process: Digital_Form: Digital_Transfer_Information: Format_Name: Microsoft Excel format, comma-delimited text, Microsoft Word format Digital_Transfer_Option: Online_Option: Computer_Contact_Information: Network_Address: Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P147MDIE/data/Foraminifera_LA-ICPMS_data.zip Fees: None Metadata_Reference_Information: Metadata_Date: 20250422 Metadata_Contact: Contact_Information: Contact_Organization_Primary: Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center Contact_Person: USGS SPCMSC Data Management Contact_Address: Address_Type: mailing and physical Address: 600 4th Street South City: Saint Petersburg State_or_Province: FL Postal_Code: 33701 Country: United States Contact_Voice_Telephone: 727-502-8000 Contact_Electronic_Mail_Address: gs-g-spcmsc_data_inquiries@usgs.gov Metadata_Standard_Name: FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata Metadata_Standard_Version: FGDC-STD-001.1-1999