Field Activity 09CDS02

Identifier 09CDS02
Purpose To compare the shifts in coral-associated microbial communities between healthy and diseased corals, in multiple species, across two National Parks (VIIS and DRTO)
Location Dry Tortugas National Park, FL
Summary Samples (2 x 2-cm hole punches) of coral tissue were collected from Montastraea annularis (five healthy colonies and three colonies affected by white plague) located at Little Africa reef near Loggerhead Key. Samples (2 x 2-cm hole punches) of coral tissue were collected from Siderastrea siderea (five healthy colonies and five colonies affected by dark spot) also located at Loggerhead Key. All tissue samples were flash frozen in liquid nitrogen and returned to the USGS St. Pete laboratory for DNA extraction and later microarray analysis.
Info derived Microbial community structure and taxonomic shifts
Comments permit DRTO-2009-SCI-0018 (study DRTO-00074)
Platform
Fort Jefferson
Halimeda
Itinerary
Start Key West, FL 2009-08-02
End Key West, FL 2009-08-15
Bounds
West -83.0755
East -82.82158049
North 24.71043268
South 24.53187618

Personnel

Organization
600 Fourth Street South
St. Petersburg, FL33701-4846
(727) 502-8000
Principal investigators Christina A Kellogg
Crew members
Billy J Reynolds
Scientist, Staff
David G Zawada
Scientist, Staff
Information specialist(s)
Christina A Kellogg
Specialist, Information

Data types and categories

Data category: Imagery, Sampling
Data type: Photo, Biology

Equipment used

Equipment Usage description Data types Datasets
Camera, Digital, Hand Held Photo (no data reported)
Sterile Hole Punch Biology 4

Datasets

Datasets produced in this activity

Dataset name Equipment Description Dataset contact
09CDS02-SB-pd-1 Sterile Hole Punch processed micro-array data on fluorescence of diseased and healthy Montastraea/Orbicella at DRTO Christina A Kellogg
09CDS02-SB-pd-2 Sterile Hole Punch processed micro-array data on fluorescence of diseased and healthy Siderastrea siderea at DRTO Christina A Kellogg
09CDS02-SB-rd-1 Sterile Hole Punch Raw data for each Montastraea/Orbicella sample collected from DRTO Christina A Kellogg
09CDS02-SB-rd-2 Sterile Hole Punch Raw data for each Siderastrea siderea sample collected from DRTO Christina A Kellogg

Publications

Samples collected during this field activity