Microbial Ecology of the Floridan Aquifer near Okeechobee, FL

Frequently anticipated questions:

• What does this data set describe?
  1. How might this data set be cited?
  2. What geographic area does the data set cover?
  3. What does it look like?
  4. Does the data set describe conditions during a particular time period?
  5. What is the general form of this data set?
  6. How does the data set represent geographic features?
  7. How does the data set describe geographic features?
• Who produced the data set?
  1. Who are the originators of the data set?
  2. Who also contributed to the data set?
  3. To whom should users address questions about the data?
• Why was the data set created?
• How was the data set created?
  1. From what previous works were the data drawn?
  2. How were the data generated, processed, and modified?
  3. What similar or related data should the user be aware of?
• How reliable are the data; what problems remain in the data set?
  1. How well have the observations been checked?
  2. How accurate are the geographic locations?
  3. How accurate are the heights or depths?
  4. Where are the gaps in the data? What is missing?
  5. How consistent are the relationships among the data, including topology?
• How can someone get a copy of the data set?
  1. Are there legal restrictions on access or use of the data?
  2. Who distributes the data?
  3. What's the catalog number I need to order this data set?
  4. What legal disclaimers am I supposed to read?
  5. How can I download or order the data?
• Who wrote the metadata?

What does this data set describe?

1. How might this data set be cited?
   Lisle, John T., 20200720, Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: U.S. Geological Survey Data Release doi:10.5066/P9EOM5RC, U.S. Geological Survey, St. Petersburg, FL.

   Online Links:

   • https://doi.org/10.5066/P9EOM5RC

   This is part of the following larger work.
   Lisle, John T., 2020, Nutrient Removal and Uptake by Native Planktonic and Biofilm Bacterial Communities in An Anaerobic Aquifer: Frontiers in Microbiology, Lausanne, Switzerland.

   Online Links:

   • https://doi.org/10.3389/fmicb.2020.01765

2. What geographic area does the data set cover?

   West_Bounding_Coordinate: -80.9280
   East_Bounding_Coordinate: -80.8072
   North_Bounding_Coordinate: 27.2009
   South_Bounding_Coordinate: 27.0885
   

3. What does it look like?
4. Does the data set describe conditions during a particular time period?

   Beginning_Date: 01-Jan-2018

   Ending_Date: 01-Oct-2019

   Currentness_Reference:

   ground condition

5. What is the general form of this data set?

   Geospatial_Data_Presentation_Form: Tabular digital data
   

6. How does the data set represent geographic features?
   1. How are geographic features stored in the data set?
      
   2. What coordinate system is used to represent geographic features?
      
7. How does the data set describe geographic features?

   Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.csv, Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.xlsx

   These files include data associated with the analysis of microbial nutrient uptake observed in samples collected from the Upper Floridan Aquifer. (Source: USGS)

   Geochemical parameter

   Groundwater geochemical parameters measured at the well head. (Source: USGS) Temperature, pH, Oxidation Reduction Potential, Specific conductance, Turbidity, Color, Total dissolved solids, Total Alkalinity (as CaCO3), Aluminum, Barium, Boron, Bromide, Calcium, Chloride, Copper, Fluoride, Iron, Magnesium, Manganese, Potassium, Silica, Sodium, Sulfate, Sulfide, Zinc, NO2-N, NO3-N, NH3-N, Total PO4-P, Ortho PO4-P, Total organic carbon, Dissolved organic carbon

   Geochemical parameter units

   Geochemical parameter units (Source: USGS)

   Value	Definition
   degrees Celsius	Temperature units
   not applicable	There are no units for pH
   millivolts	Oxidation reduction potential units
   microsiemens/cm	Specific conductance units
   nephelometric turbidity units (NTU)	Turbidity units
   platinum cobalt units (PCU)	Color units
   mg/L	Concentration units as milligrams per liter

   Geochemical parameter mean and standard deviation

   Arithmetic mean with 1 standard deviation value for each of the geochemical parameters. (Source: USGS) The actual measured values for each of the respective geochemical parameters.

   Time (hrs) Nitrogen Planktonic

   Experimental sample collection times, in hours, for the nitrogen removal experiment. (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	169.75

   NO3-N (mg/L) Planktonic

   The concentrations of nitrate-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0414
   Maximum:	0.4231

   NO2-N (mg/L) Planktonic

   The concentrations of nitrite-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0003
   Maximum:	0.3678

   NH4-N (mg/L) Planktonic

   The concentrations of ammonia-nitrogen observed in samples collected at the listed time points in Figure 2A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.4267
   Maximum:	0.4342

   Time (hrs) Nitrogen Biofilm

   Experimental sample collection times, in hours, for the nitrogen removal experiment. (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	169.25

   NO3-N (mg/L) Biofilm

   The concentrations of nitrate-nitrogen observed in samples collected at the listed time points in Figure 2AB (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0042
   Maximum:	0.3938

   NO2-N (mg/L) Biofilm

   The concentrations of nitrite-nitrogen observed in samples collected at the listed time points in Figure 2B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0006
   Maximum:	0.1241

   NH4-N (mg/L) Biofilm

   The concentrations of ammonia-nitrogen observed in samples collected at the listed time points in Figure 2B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.4186
   Maximum:	0.4489

   Time (hrs) Phosphorus Planktonic

   Experimental sample collection times, in hours, for the phosphorus uptake experiment graphed in Figure 3A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	6.0

   PO4-P (mg/L) Planktonic

   The concentrations of phosphorus observed in samples collected at the listed time points in Figure 3A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	0.0032

   Time (hrs) Phosphorus Biofilm

   Experimental sample collection times, in hours, for the phosphorus uptake experiment graphed in Figure 3B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	6.0

   PO4-P (mg/L) Biofilm

   The concentrations of phosphorus observed in samples collected at the listed time points in Figure 3B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	0.477

   Time (hrs) Carbon Planktonic

   Experimental sample collection times, in hours, for the carbon uptake experiment graphed in Figure 4A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	123.0

   CO2 (mg/L) Planktonic

   The concentrations of carbon dioxide observed in samples collected at the listed time points in Figure 4A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	0.24479

   Biomass (mg/L) Planktonic

   The concentrations of carbon observed in the bacterial biomass samples collected at the listed time points in Figure 4A (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	0.40887

   Time (hrs) Carbon Biofilm

   Experimental sample collection times, in hours, for the carbon uptake experiment graphed in Figure 4B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	122.0

   CO2 (mg/L) Biofilm

   The concentrations of carbon dioxide observed in samples collected at the listed time points in Figure 4B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	0.38774

   Biomass (mg/L) Biofilm

   The concentrations of carbon observed in the bacterial biomass samples collected at the listed time points in Figure 4B (Lisle, 2020). (Source: USGS)

   Range of values
   Minimum:	0.0
   Maximum:	1.9413

Who produced the data set?

1. Who are the originators of the data set? (may include formal authors, digital compilers, and editors)
   • John T. Lisle
2. Who also contributed to the data set?

   USGS and South Florida Water Management District.

3. To whom should users address questions about the data?
   John T Lisle

   Southeast Region: ST. PETE COASTAL & MARINE SC

   Research Microbiologist

   600 4Th Street South

   St. Petersburg, FL
   

   United States

   727-502-8140 (voice)

   jlisle@usgs.gov

Why was the data set created?

Data were collected to quantify the nutrient uptake rates under native groundwater geochemical conditions.

How was the data set created?

1. From what previous works were the data drawn?
2. How were the data generated, processed, and modified?

   Date: 01-Aug-2019 (process 1 of 2)

   Between 2018 and 2019 groundwater samples were collected from a SFWMD monitoring well located at a study site near Lake Okeechobee. Samples were dosed with standardized solutions of nitrogen (KNO3), phosphorus (K2PO4) and carbon (Na-acetate) then sampled over a period of time to quantify the rates at which the native planktonic and biofilm-associated bacteria removed these nutrients and either incorporated them into bacterial biomass and/or carbon dioxide. The data from the nitrogen removal experiments were generated on a Seal Analytical Auto Analyzer 3, while the data from the phosphorus and carbon uptake experiments were generated on a Perkin Elmer TriCarb liquid scintillation counter following the measurement of samples containing 32P (as phosphate) and 14C (as acetate).

   Date: 13-Oct-2020 (process 2 of 2)

   Added keywords section with USGS persistent identifier as theme keyword. Person who carried out this activity:
   

   U.S. Geological Survey

   Attn: VeeAnn A. Cross

   Marine Geologist

   384 Woods Hole Road

   Woods Hole, MA
   

   508-548-8700 x2251 (voice)

   508-457-2310 (FAX)

   vatnipp@usgs.gov

3. What similar or related data should the user be aware of?

How reliable are the data; what problems remain in the data set?

1. How well have the observations been checked?
   No formal attribute accuracy tests were conducted.
2. How accurate are the geographic locations?
   No formal positional accuracy tests were conducted.
3. How accurate are the heights or depths?
   No formal positional accuracy tests were conducted.
4. Where are the gaps in the data? What is missing?
   Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
5. How consistent are the relationships among the observations, including topology?
   No formal logical accuracy tests were conducted.

How can someone get a copy of the data set?

Are there legal restrictions on access or use of the data?

Access_Constraints: None
Use_Constraints: None

1. Who distributes the data set? (Distributor 1 of 1)
   
   John T Lisle

   Southeast Region: ST. PETE COASTAL & MARINE SC

   Research Microbiologist

   600 4Th Street South

   St. Petersburg, FL
   

   United States

   727-502-8140 (voice)

   jlisle@usgs.gov
2. What's the catalog number I need to order this data set?
3. What legal disclaimers am I supposed to read?

   Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

4. How can I download or order the data?
   • Availability in digital form:

     Data format:	CSV, XLXS
     Network links:	https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.zip

   • Cost to order the data: None

Who wrote the metadata?

Dates:

Last modified: 13-Oct-2020

Metadata author:

John T Lisle

Southeast Region: ST. PETE COASTAL & MARINE SC

Research Microbiologist

600 4Th Street South

St. Petersburg, FL

United States

727-502-8140 (voice)

jlisle@usgs.gov

Metadata standard:

Content Standard for Digital Geospatial Metadata (FGDC-STD-001-1998)