Other ID: none
Status: Completed
Organization(s): USGS, St. Petersburg Coastal and Marine Science Center
Funding Program(s):
Principal Investigator(s): Christina Kellogg
Affiliate Investigator(s):
Information Specialist(s): Christina Kellogg
Data Type(s): Imagery: Photo, Sampling: Biology
Scientific Purpose/Goals: To compare the shifts in coral-associated microbial communities between healthy and diseased corals, in multiple species, across two National Parks (VIIS and DRTO)
Vehicle(s):
Start Port/Location: St. John, USVI
End Port/Location: St. John, USVI
Start Date: 2009-07-15
End Date: 2009-07-27
Equipment Used: Sterile Hole Punch, Camera, Digital, Hand Held
Information to be Derived: Microbial community structure and taxonomic shifts
Summary of Activity and Data Gathered: Samples (2 x 2-cm hole punches) of coral tissue were collected from Montastraea annularis (five healthy colonies and five colonies affected by white plague) located at Tektite reef in Greater Lameshur Bay. Samples (2 x 2-cm hole punches) of coral tissue were collected from Siderastrea siderea (five healthy colonies and five colonies affected by dark spot) located at Hawksnest Bay. All tissue samples were flash frozen in liquid nitrogen and returned to the USGS St. Pete laboratory for DNA extraction and later microarray analysis.
Staff: Virginia Garrison
Affiliate Staff:
Notes: Platform: shore diving or whalers, as needed.
permit VIIS-2008-SCI-0033 (study VIIS-08033)
Location:
U.S. Virgin Islands
| Boundaries | |||
|---|---|---|---|
| North: 18.0886405 | South: 17.44339007 | West: -65.1365 | East: -64.26857314 |
Platform(s):
 Whaler |
Baker, E.J., Kellogg, C.A., 2014, Comparison of three DNA extraction kits to establish maximum yield and quality of coral-associated microbial DNA: U.S. Geological Survey Open-File Report 2014-1066, 17 p., https://doi.org/10.3133/ofr20141066.
Kellogg C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Andersen, G.L., 2014, Comparing Bacterial Community Composition of Healthy and Dark Spot-Affected Siderastrea siderea in Florida and the Caribbean: PLoS ONE, v. 9 no. 10, doi: 10.1371/journal.pone.0108767.
Kellogg, C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Zawada, D.G., Andersen, G.L., 2013, Comparing bacterial community composition between healthy and white plague-like disease states in Orbicella annularis using PhyloChipTM G3 microarrays: PLOS One, v. 8, e79801 p., doi: 10.1371/journal.pone.0079801.
| Survey Equipment | Survey Info | Data Type(s) | Data Collected |
|---|---|---|---|
| Sterile Hole Punch | --- | Biology | 09CDS01-SB-rd-1 (Raw data for each Montastraea/Orbicella sample collected from USVI) 09CDS01-SB-rd-2 (Raw data for each Siderastrea siderea sample collected from USVI) 09CDS01-SB-pd-1 (processed micro-array data on fluorescence of diseased and healthy Montastraea/Orbicella at USVI) 09CDS01-SB-pd-2 (processed micro-array data on fluorescence of diseased and healthy Siderastrea siderea at USVI ) |
| Camera, Digital, Hand Held | --- | Photo |
