Other ID: none
Status: Completed
Organization(s): USGS, St. Petersburg Coastal and Marine Science Center
Funding Program(s):
Principal Investigator(s): Christina Kellogg
Affiliate Investigator(s):
Information Specialist(s): Christina Kellogg
Data Type(s): Imagery: Photo, Sampling: Biology
Scientific Purpose/Goals: To compare the shifts in coral-associated microbial communities between healthy and diseased corals, in multiple species, across two National Parks (VIIS and DRTO)
Vehicle(s):
Start Port/Location: Key West, FL
End Port/Location: Key West, FL
Start Date: 2009-08-02
End Date: 2009-08-15
Equipment Used: Camera, Digital, Hand Held, Sterile Hole Punch
Information to be Derived: Microbial community structure and taxonomic shifts
Summary of Activity and Data Gathered: Samples (2 x 2-cm hole punches) of coral tissue were collected from Montastraea annularis (five healthy colonies and three colonies affected by white plague) located at Little Africa reef near Loggerhead Key. Samples (2 x 2-cm hole punches) of coral tissue were collected from Siderastrea siderea (five healthy colonies and five colonies affected by dark spot) also located at Loggerhead Key. All tissue samples were flash frozen in liquid nitrogen and returned to the USGS St. Pete laboratory for DNA extraction and later microarray analysis.
Staff: Billy Reynolds, David Zawada
Affiliate Staff:
Notes: permit DRTO-2009-SCI-0018 (study DRTO-00074)
Location:
Dry Tortugas National Park, FL
Boundaries | |||
---|---|---|---|
North: 24.71043268 | South: 24.53187618 | West: -83.0755 | East: -82.82158049 |
Platform(s):
Fort Jefferson | Halimeda |
Baker, E.J., Kellogg, C.A., 2014, Comparison of three DNA extraction kits to establish maximum yield and quality of coral-associated microbial DNA: U.S. Geological Survey Open-File Report 2014-1066, 17 p., https://doi.org/10.3133/ofr20141066.
Kellogg C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Andersen, G.L., 2014, Comparing Bacterial Community Composition of Healthy and Dark Spot-Affected Siderastrea siderea in Florida and the Caribbean: PLoS ONE, v. 9 no. 10, doi: 10.1371/journal.pone.0108767.
Kellogg, C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Zawada, D.G., Andersen, G.L., 2013, Comparing bacterial community composition between healthy and white plague-like disease states in Orbicella annularis using PhyloChipTM G3 microarrays: PLOS One, v. 8, e79801 p., doi: 10.1371/journal.pone.0079801.
Kellogg, C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Zawada, D.G., Andersen, G.L., 2012, PhyloChip(TM) microarray comparison of sampling methods used for coral microbial ecology: Journal of Microbiological Methods, v. 88, pp. 103–109.
Ross, S.W., Demopoulos, A.W.J., Kellogg, C.A., Morrison, C.L., Nizinski, M.S., Ames, C.L., Casazza, T.L., Gualtieri, D., Kovacs, K., McClain, J.P., Quattrini, A.M., Roa-Varòn, A.Y., Thaler, A.D., 2012, Deepwater Program: Studies of Gulf of Mexico lower continental slope communities related to chemosynthetic and hard substrate habitats: U.S. Geological Survey Open-File Report 2012-1032, 301 p., https://doi.org/10.3133/ofr20121032.
Survey Equipment | Survey Info | Data Type(s) | Data Collected |
---|---|---|---|
Camera, Digital, Hand Held | --- | Photo | |
Sterile Hole Punch | --- | Biology | 09CDS02-SB-rd-1 (Raw data for each Montastraea/Orbicella sample collected from DRTO) 09CDS02-SB-rd-2 (Raw data for each Siderastrea siderea sample collected from DRTO) 09CDS02-SB-pd-1 (processed micro-array data on fluorescence of diseased and healthy Montastraea/Orbicella at DRTO) 09CDS02-SB-pd-2 (processed micro-array data on fluorescence of diseased and healthy Siderastrea siderea at DRTO) |