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USGS Coastal and Marine Geology Program

Field Activity Details for field activity 09CDS02

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Other ID: none

Status: Completed

Organization(s): USGS, St. Petersburg Coastal and Marine Science Center

Funding Program(s):

Principal Investigator(s): Christina Kellogg

Affiliate Investigator(s):

Information Specialist(s): Christina Kellogg

Data Type(s): Imagery: Photo, Sampling: Biology

Scientific Purpose/Goals: To compare the shifts in coral-associated microbial communities between healthy and diseased corals, in multiple species, across two National Parks (VIIS and DRTO)

Vehicle(s):

Start Port/Location: Key West, FL

End Port/Location: Key West, FL

Start Date: 2009-08-02

End Date: 2009-08-15

Equipment Used: Camera, Digital, Hand Held, Sterile Hole Punch

Information to be Derived: Microbial community structure and taxonomic shifts

Summary of Activity and Data Gathered: Samples (2 x 2-cm hole punches) of coral tissue were collected from Montastraea annularis (five healthy colonies and three colonies affected by white plague) located at Little Africa reef near Loggerhead Key. Samples (2 x 2-cm hole punches) of coral tissue were collected from Siderastrea siderea (five healthy colonies and five colonies affected by dark spot) also located at Loggerhead Key. All tissue samples were flash frozen in liquid nitrogen and returned to the USGS St. Pete laboratory for DNA extraction and later microarray analysis.

Staff: Billy Reynolds, David Zawada

Affiliate Staff:

Notes: permit DRTO-2009-SCI-0018 (study DRTO-00074)

Location:

Dry Tortugas National Park, FL

Boundaries
North: 24.71043268 South: 24.53187618 West: -83.0755 East: -82.82158049

Platform(s):

photo of Fort Jefferson
Fort Jefferson
photo of Halimeda
Halimeda

Publications

Baker, E.J., Kellogg, C.A., 2014, Comparison of three DNA extraction kits to establish maximum yield and quality of coral-associated microbial DNA: U.S. Geological Survey Open-File Report 2014-1066, 17 p., https://doi.org/10.3133/ofr20141066.

Kellogg C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Andersen, G.L., 2014, Comparing Bacterial Community Composition of Healthy and Dark Spot-Affected Siderastrea siderea in Florida and the Caribbean: PLoS ONE, v. 9 no. 10, doi: 10.1371/journal.pone.0108767.

Kellogg, C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Gray, M.A., Zawada, D.G., Andersen, G.L., 2013, Comparing bacterial community composition between healthy and white plague-like disease states in Orbicella annularis using PhyloChipTM G3 microarrays: PLOS One, v. 8, e79801 p., doi: 10.1371/journal.pone.0079801.

Kellogg, C.A., Piceno, Y.M., Tom, L.M., DeSantis, T.Z., Zawada, D.G., Andersen, G.L., 2012, PhyloChip(TM) microarray comparison of sampling methods used for coral microbial ecology: Journal of Microbiological Methods, v. 88, pp. 103–109.

Ross, S.W., Demopoulos, A.W.J., Kellogg, C.A., Morrison, C.L., Nizinski, M.S., Ames, C.L., Casazza, T.L., Gualtieri, D., Kovacs, K., McClain, J.P., Quattrini, A.M., Roa-Varòn, A.Y., Thaler, A.D., 2012, Deepwater Program: Studies of Gulf of Mexico lower continental slope communities related to chemosynthetic and hard substrate habitats: U.S. Geological Survey Open-File Report 2012-1032, 301 p., https://doi.org/10.3133/ofr20121032.

Portals/Viewers

Data Acquired

Survey EquipmentSurvey InfoData Type(s)Data Collected
Camera, Digital, Hand Held --- Photo
Sterile Hole Punch --- Biology
09CDS02-SB-rd-1 (Raw data for each Montastraea/Orbicella sample collected from DRTO)
09CDS02-SB-rd-2 (Raw data for each Siderastrea siderea sample collected from DRTO)
09CDS02-SB-pd-1 (processed micro-array data on fluorescence of diseased and healthy Montastraea/Orbicella at DRTO)
09CDS02-SB-pd-2 (processed micro-array data on fluorescence of diseased and healthy Siderastrea siderea at DRTO)

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