Field Activity 09DCM02

Identifier 09DCM02
Purpose To characterize the microbial community associated with the deep-sea coral Lophelia pertusa
Location Gulf of Mexico
Summary Two colonies of the deep-sea coral Lophelia pertusa were sampled from the Ewing wreck and two colonies from the Gulf Penn wreck. Coral tissue was preserved in RNAlater(r) for microbial DNA extraction in St. Petersburg.
Info derived Bacterial-community species composition and presence of specific functional genes
Comments Shop Time: Kellogg used the shop to construct two milkcrate-based samplers (4 days, utilized saw, drill, hand tools). Dave Wegner drilled and shaped the container caps (~1 hour).
Platform
ROV Jason
Two-body ROV system. A 10-kilometer (6-mile) reinforced fiber-optic cable delivers electrical power and commands from the ship through Medea and down to Jason, which then returns data and live video imagery. Medea serves as a shock absorber, buffering Jason from the movements of the ship, while providing lighting and a bird’s eye view of the ROV during seafloor operations. Jason is equipped with sonars, video and still imaging systems, lighting, and numerous sampling systems.
Ronald H. Brown
Length: 274 feet; Beam: 53 feet
Itinerary
Start Key West, FL 2009-08-18
End Pensacola, FL 2009-09-12
Bounds
West -99.447
East -78.97827983
North 32.66815435
South 23.225394
Topic 26
Marine operations Yes

Personnel

Organization
600 Fourth Street South
St. Petersburg, FL33701-4846
(727) 502-8000
Principal investigators Christina A Kellogg
Crew members
Christina A Kellogg
Scientist, Staff
Information specialist(s)
Christina A Kellogg
Specialist, Information

Data types and categories

Data category: Biological Field Study, Sampling
Data type: Experiments (biological), Surveys (biological), Biology

Equipment and datasets

Equipment or measuring device
Kellogg Sampler
Experiments (biological), Surveys (biological), Biology
3
Lophelia_metagenomes_raw_data_1.zip(38938)
Description Zip file containing raw data for CK1-CK6, and MIMS sampling metadata
Data types
Surveys (biological)
Format comma-delimited text; FASTQ
Amount 17.5 GB
Digital location \\gs\StPetersburgFL-G\Ecosystem_and_Environmental_Studies\Archive\Publications\DR_P9AA0H3B
Public? Yes
Reviewed? Yes
Public URL https://doi.org/10.5066/P9AA0H3B
Metadata https://cmgds.marine.usgs.gov/metadata/spcmsc/Lophelia_metagenome_metadata.xml
Contact
Lophelia_metagenomes_raw_data_2.zip(38941)
Description Zip file containing raw data for CK7-CK12, PhiX control, and MIMS sampling metadata.
Data types
Surveys (biological)
Format comma-delimited text; FASTQ
Amount 10.3 GB
Digital location \\gs\StPetersburgFL-G\Ecosystem_and_Environmental_Studies\Archive\Publications\DR_P9AA0H3B
Public? Yes
Reviewed? Yes
Public URL https://doi.org/10.5066/P9AA0H3B
Metadata https://cmgds.marine.usgs.gov/metadata/spcmsc/Lophelia_metagenome_metadata.xml
Contact
Lophelia_raw_data.zip(14683)
Description Zip file containing raw data (.fna, .qual, .sff) and MIMARKS metadata.
Format Physical sample
Amount 2.6 GB
Digital location .fna, .qual, .sff
Supported? No
Public? Yes
Reviewed? Yes
Process? No
Public URL https://doi.org/10.5066/F7M32SXM
Metadata https://cmgds.marine.usgs.gov/metadata/spcmsc/Cold_water_coral_microbiomes_Lophelia_pertusa_from_Gulf_of_Mexico_and_Atlantic_Ocean_raw_data.xml
Contact

Description and usage history of this equipment

Publications

Samples collected during this field activity

Similar field activities carried out by this science center