Compound specific isotope analysis of amino acids from Escanaba Trough sediments, off the coast of Northern California, USA, from May-June 2022.

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What does this data set describe?

Title:

Compound specific isotope analysis of amino acids from Escanaba Trough sediments, off the coast of Northern California, USA, from May-June 2022.

Abstract:

Stable carbon isotope ratios (d13C), stable nitrogen isotope ratios (d15N), and molar percentage data of amino acids were determined on one to five cm subsamples of sediment push cores collected from the Escanaba Trough during May to June 2022.

Supplemental_Information:

Additional information about the field activity or activities from which these data were derived is available online at:
https:// https://cmgds.marine.usgs.gov/fan_info.php?fan=2022-621-FA
Escanaba Trough: Exploring the Seafloor and Oceanic Footprints is an interagency effort involving the National Oceanic and Atmospheric Administration, the U.S. Geological Survey, and the Bureau of Ocean Energy Management. Funding is acknowledged from NOAA Ocean Exploration via its Ocean Exploration Fiscal Year 2019 Funding Opportunity and through the National Oceanographic Partnership Program (NOPP), from the USGS Coastal and Marine Hazards and Resources Enhanced Funding Opportunities, and from the Bureau of Ocean Energy Management through Interagency Agreement M19PG00021 with the United States Geological Survey.
Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

How might this data set be cited?
Ianiri, Hope L., and Prouty, Nancy G., 20250218, Compound specific isotope analysis of amino acids from Escanaba Trough sediments, off the coast of Northern California, USA, from May-June 2022.: data release DOI:10.5066/P13XVXQC, U.S. Geological Survey, Pacific Coastal and Marine Science Center, Santa Cruz, California.
Online Links:
- https://doi.org/10.5066/P13XVXQC
This is part of the following larger work.
Ianiri, Hope L., Prouty, Nancy G., Campbell, Pamela L., Gartman, Amy, Adamczyk, Katlin B., and Favela, Jaycee J., 2025, Sedimentary organic geochemistry data from Escanaba Trough, off the coast of northern California, collected May-June 2022.: data release DOI:10.5066/P13XVXQC, U.S. Geological Survey, Pacific Coastal and Marine Science Center, Santa Cruz, CA.
Online Links:
- https://doi.org/10.5066/P13XVXQC
Other_Citation_Details:
Suggested Citation: Ianiri, H.L., Prouty, N.G., Campbell, P.L., Gartman, A., Adamczyk, K.B., and Favela, J.J., 2025, Sedimentary organic geochemistry data from Escanaba Trough, off the coast of northern California, collected May-June 2022: U.S. Geological Survey data release, https://doi.org/10.5066/P13XVXQC.
What geographic area does the data set cover?
West_Bounding_Coordinate: -127.53618710
East_Bounding_Coordinate: -127.44714953
North_Bounding_Coordinate: 41.08222461
South_Bounding_Coordinate: 40.69569198
What does it look like?
Does the data set describe conditions during a particular time period?

Beginning_Date: 31-May-2022

Ending_Date: 11-Jun-2022

Currentness_Reference:

Ground condition at time data were collected.
What is the general form of this data set?
Geospatial_Data_Presentation_Form: comma-delimited text
How does the data set represent geographic features?
1. How are geographic features stored in the data set?
  This is a point data set. It contains the following vector data types (SDTS terminology):
  - point (12)
2. What coordinate system is used to represent geographic features?
  Horizontal positions are specified in geographic coordinates, that is, latitude and longitude. Latitudes are given to the nearest 0.00000001. Longitudes are given to the nearest 0.0000001. Latitude and longitude values are specified in Decimal degrees. The horizontal datum used is D_WGS_1984.
  The ellipsoid used is WGS84.
  The semi-major axis of the ellipsoid used is 6378137.0000.
  The flattening of the ellipsoid used is 1/298.2572.

How does the data set describe geographic features?

EscanabaTrough_CSIAA.csv

Table of d13C, d15N, and molar percentage data of amino acids data for subsamples of sediment push cores. (Source: producer defined)

Cruise_ID

Lists the alphanumeric cruise identifier for the expedition during which the sample was collected. (Source: producer defined)

Value	Definition
TN403	Cruise identification affiliated with Field Activity 2022-621-FA.

Sample_ID

Name of sample (Source: producer defined) The sample names include a dive number (J2, denoting Jason2, followed by a number), push core number (PC, denoting push core, followed by a number) and the sediment depth horizon (X-Y).

Latitude

Latitude of sample in decimal degrees (Source: producer defined)

Range of values
Minimum:	40.69572818
Maximum:	41.08224004
Units:	decimal degrees
Resolution:	0.00000001

Longitude

Longitude of sample in decimal degrees (Source: producer defined)

Range of values
Minimum:	-127.5282233
Maximum:	-127.4470967
Units:	decimal degrees
Resolution:	0.0000001

Water_depth_m

Water depth in meters at which the sample was collected. (Source: producer defined)

Range of values
Minimum:	3223
Maximum:	3235
Units:	meters
Resolution:	1

Location

Location where the sample was collected (Source: producer defined)

Value	Definition
Central Hill	Text descriptor of sampling site as defined in the literature
Edifice Rex East	Text descriptor of sampling site as defined in the literature
SESCA S3A	Text descriptor of sampling site as defined in the literature

sediment_depth_top_cm

top depth of core subsample. For example, a sediment core slice from 0-1 cm would have a top depth of 0 centimeters and a bottom depth of 1 centimeter. (Source: producer defined)

Range of values
Minimum:	0
Maximum:	18
Units:	centimeters
Resolution:	1

sediment_depth_bottom_cm

bottom depth of core subsample. For example, a sediment core slice from 0-1 cm would have a top depth of 0 centimeters and a bottom depth of 1 centimeter. (Source: producer defined)

Range of values
Minimum:	1
Maximum:	20
Units:	centimeters
Resolution:	1

Ala_d13C

ratio of stable carbon 13/12 isotopes of alanine (Source: producer defined)

Range of values
Minimum:	-28.9
Maximum:	-19.7
Units:	per mil
Resolution:	0.1

Gly_d13C

ratio of stable carbon 13/12 isotopes of glycine (Source: producer defined)

Range of values
Minimum:	-18.9
Maximum:	-10.3
Units:	per mil
Resolution:	0.1

Thr_d13C

ratio of stable carbon 13/12 isotopes of threonine (Source: producer defined)

Range of values
Minimum:	-25.2
Maximum:	-10.1
Units:	per mil
Resolution:	0.1

Ser_d13C

ratio of stable carbon 13/12 isotopes of serine. -9999 indicates not reported due to co-elution with an additional peak. (Source: producer defined)

Range of values
Minimum:	-9999
Maximum:	-9999
Units:	per mil

Val_d13C

ratio of stable carbon 13/12 isotopes of valine. (Source: producer defined)

Range of values
Minimum:	-41.6
Maximum:	-24.8
Units:	per mil
Resolution:	0.1

Leu_d13C

ratio of stable carbon 13/12 isotopes of leucine. (Source: producer defined)

Range of values
Minimum:	-44.9
Maximum:	-25.7
Units:	per mil
Resolution:	0.1

Ile_d13C

ratio of stable carbon 13/12 isotopes of isoleucine. (Source: producer defined)

Range of values
Minimum:	-40.0
Maximum:	-21.0
Units:	per mil
Resolution:	0.1

Pro_d13C

ratio of stable carbon 13/12 isotopes of proline. (Source: producer defined)

Range of values
Minimum:	-47.1
Maximum:	-16.2
Units:	per mil
Resolution:	0.1

Asp_d13C

ratio of stable carbon 13/12 isotopes of aspartic acid and asparagine. (Source: producer defined)

Range of values
Minimum:	-23.5
Maximum:	-10.0
Units:	per mil
Resolution:	0.1

Glu_d13C

ratio of stable carbon 13/12 isotopes of glutamic acid and glutamine. (Source: producer defined)

Range of values
Minimum:	-34.4
Maximum:	-15.7
Units:	per mil
Resolution:	0.1

Phe_d13C

ratio of stable carbon 13/12 isotopes of phenylalanine. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	-41.2
Maximum:	-24.3
Units:	per mil
Resolution:	0.1

Tyr_d13C

ratio of stable carbon 13/12 isotopes of tyrosine. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	-41.6
Maximum:	-28.2
Units:	per mil
Resolution:	0.1

Lys_d13C

ratio of stable carbon 13/12 isotopes of lysine. (Source: producer defined)

Range of values
Minimum:	-31.4
Maximum:	-13.1
Units:	per mil
Resolution:	0.1

Ala_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of alanine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.5
Units:	per mil
Resolution:	0.1

Gly_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of glycine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.6
Units:	per mil
Resolution:	0.1

Thr_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of threonine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.9
Units:	per mil
Resolution:	0.1

Ser_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of serine measured by triplicate injections -9999 indicates not reported due to co-elution with an additional peak. (Source: producer defined)

Range of values
Minimum:	-9999
Maximum:	-9999
Units:	per mil

Val_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of valine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.2
Maximum:	3.2
Units:	per mil
Resolution:	0.1

Leu_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of leucine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.3
Units:	per mil
Resolution:	0.1

Ile_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of isoleucine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.4
Units:	per mil
Resolution:	0.1

Pro_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of proline measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	3.8
Units:	per mil
Resolution:	0.1

Asp_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of aspartic acid and asparagine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.7
Units:	per mil
Resolution:	0.1

Glu_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of glutamic acid and glutamine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.2
Maximum:	0.9
Units:	per mil
Resolution:	0.1

Phe_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of phenylalanine measured by triplicate injections. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.2
Maximum:	2.2
Units:	per mil
Resolution:	0.1

Tyr_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of tyrosine measured by triplicate injections. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.3
Maximum:	4.0
Units:	per mil
Resolution:	0.1

Lys_SD_d13C

standard deviation of the ratio of stable carbon 13/12 isotopes of lysine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	4.3
Units:	per mil
Resolution:	0.1

Ala_molC

molar percentage of alanine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	10.0
Maximum:	18.2
Units:	percent
Resolution:	0.1

Gly_molC

molar percentage of glycine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	21.2
Maximum:	38.8
Units:	percent
Resolution:	0.1

Thr_molC

molar percentage of threonine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.2
Maximum:	7.4
Units:	percent
Resolution:	0.1

Ser_molC

molar percentage of serine as calculated from d13C isotope ratio mass spectrometry runs. -9999 indicates not reported due to co-elution with an additional peak. (Source: producer defined)

Range of values
Minimum:	-9999
Maximum:	-9999
Units:	percent

Val_molC

molar percentage of valine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.6
Maximum:	8.2
Units:	percent
Resolution:	0.1

Leu_molC

molar percentage of leucine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.9
Maximum:	9.0
Units:	percent
Resolution:	0.1

Ile_molC

molar percentage of isoleucine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	1.6
Maximum:	4.9
Units:	percent
Resolution:	0.1

Pro_molC

molar percentage of proline as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	6.2
Maximum:	12.3
Units:	percent
Resolution:	0.1

Asp_molC

molar percentage of aspartic acid and asparagine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	6.3
Maximum:	15.2
Units:	percent
Resolution:	0.1

Glu_molC

molar percentage of glutamic acid and glutamine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	4.5
Maximum:	9.2
Units:	percent
Resolution:	0.1

Phe_molC

molar percentage of phenylalanine as calculated from d13C isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	1.1
Maximum:	3.6
Units:	percent
Resolution:	0.1

Tyr_molC

molar percentage of tyrosine as calculated from d13C isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.6
Maximum:	1.6
Units:	percent
Resolution:	0.1

Lys_molC

molar percentage of lysine as calculated from d13C isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	2.9
Maximum:	7.3
Units:	percent
Resolution:	0.1

Ala_d15N

ratio of stable nitrogen 15/14 isotopes of alanine. (Source: producer defined)

Range of values
Minimum:	8.2
Maximum:	23.8
Units:	per mil
Resolution:	0.1

Gly_d15N

ratio of stable nitrogen 15/14 isotopes of glycine. (Source: producer defined)

Range of values
Minimum:	-1.0
Maximum:	11.3
Units:	per mil
Resolution:	0.1

Thr_d15N

ratio of stable nitrogen 15/14 isotopes of threonine. (Source: producer defined)

Range of values
Minimum:	-2.1
Maximum:	8.2
Units:	per mil
Resolution:	0.1

Ser_d15N

ratio of stable nitrogen 15/14 isotopes of serine. (Source: producer defined)

Range of values
Minimum:	-2.8
Maximum:	8.6
Units:	per mil
Resolution:	0.1

Val_d15N

ratio of stable nitrogen 15/14 isotopes of valine. (Source: producer defined)

Range of values
Minimum:	6.2
Maximum:	20.8
Units:	per mil
Resolution:	0.1

Leu_d15N

ratio of stable nitrogen 15/14 isotopes of leucine. (Source: producer defined)

Range of values
Minimum:	-2.4
Maximum:	14.4
Units:	per mil
Resolution:	0.1

Ile_d15N

ratio of stable nitrogen 15/14 isotopes of isoleucine. (Source: producer defined)

Range of values
Minimum:	-1.3
Maximum:	16.4
Units:	per mil
Resolution:	0.1

Pro_d15N

ratio of stable nitrogen 15/14 isotopes of proline. (Source: producer defined)

Range of values
Minimum:	4.0
Maximum:	16.6
Units:	per mil
Resolution:	0.1

Asp_d15N

ratio of stable nitrogen 15/14 isotopes of aspartic acid and asparagine. (Source: producer defined)

Range of values
Minimum:	5.2
Maximum:	17.7
Units:	per mil
Resolution:	0.1

Glu_d15N

ratio of stable nitrogen 15/14 isotopes of glutamic acid and glutamine. (Source: producer defined)

Range of values
Minimum:	7.0
Maximum:	19.7
Units:	per mil
Resolution:	0.1

Phe_d15N

ratio of stable nitrogen 15/14 isotopes of phenylalanine. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	-1.7
Maximum:	11.7
Units:	per mil
Resolution:	0.1

Tyr_d15N

ratio of stable nitrogen 15/14 isotopes of tyrosine. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	-6.1
Maximum:	3.4
Units:	per mil
Resolution:	0.1

Lys_d15N

ratio of stable nitrogen 15/14 isotopes of lysine. (Source: producer defined)

Range of values
Minimum:	-4.9
Maximum:	9.1
Units:	per mil
Resolution:	0.1

Ala_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of alanine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.3
Maximum:	1.0
Units:	per mil
Resolution:	0.1

Gly_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of glycine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.0
Maximum:	0.7
Units:	per mil
Resolution:	0.1

Thr_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of threonine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.4
Units:	per mil
Resolution:	0.1

Ser_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of serine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.6
Units:	per mil
Resolution:	0.1

Val_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of valine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.0
Maximum:	0.7
Units:	per mil
Resolution:	0.1

Leu_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of leucine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.8
Units:	per mil
Resolution:	0.1

Ile_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of isoleucine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.2
Maximum:	2.0
Units:	per mil
Resolution:	0.1

Pro_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of proline measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.5
Units:	per mil
Resolution:	0.1

Asp_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of aspartic acid and asparagine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.1
Units:	per mil
Resolution:	0.1

Glu_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of glutamic acid and glutamine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.5
Units:	per mil
Resolution:	0.1

Phe_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of phenylalanine measured by triplicate injections. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	1.1
Units:	per mil
Resolution:	0.1

Tyr_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of tyrosine measured by triplicate injections. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.4
Maximum:	1.9
Units:	per mil
Resolution:	0.1

Lys_SD_d15N

standard deviation of the ratio of stable nitrogen 15/14 isotopes of lysine measured by triplicate injections. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.6
Units:	per mil
Resolution:	0.1

Ala_molN

molar percentage of alanine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	11.4
Maximum:	18.9
Units:	percent
Resolution:	0.1

Gly_molN

molar percentage of glycine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	19.9
Maximum:	36.0
Units:	percent
Resolution:	0.1

Thr_molN

molar percentage of threonine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.7
Maximum:	6.3
Units:	percent
Resolution:	0.1

Ser_molN

molar percentage of serine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	2.5
Maximum:	8.9
Units:	percent
Resolution:	0.1

Val_molN

molar percentage of valine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	2.6
Maximum:	6.5
Units:	percent
Resolution:	0.1

Leu_molN

molar percentage of leucine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.4
Maximum:	8.7
Units:	percent
Resolution:	0.1

Ile_molN

molar percentage of isoleucine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	1.4
Maximum:	4.2
Units:	percent
Resolution:	0.1

Pro_molN

molar percentage of proline as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	5.8
Maximum:	11.8
Units:	percent
Resolution:	0.1

Asp_molN

molar percentage of aspartic acid and asparagine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	5.8
Maximum:	13.6
Units:	percent
Resolution:	0.1

Glu_molN

molar percentage of glutamic acid and glutamine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	6.0
Maximum:	9.5
Units:	percent
Resolution:	0.1

Phe_molN

molar percentage of phenylalanine as calculated from d15N isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.6
Maximum:	3.6
Units:	percent
Resolution:	0.1

Tyr_molN

molar percentage of tyrosine as calculated from d15N isotope ratio mass spectrometry runs. -8888 indicates peak was below detection limit. (Source: producer defined)

Range of values
Minimum:	0.6
Maximum:	1.4
Units:	percent
Resolution:	0.1

Lys_molN

molar percentage of lysine as calculated from d15N isotope ratio mass spectrometry runs. (Source: producer defined)

Range of values
Minimum:	3.2
Maximum:	9.2
Units:	percent
Resolution:	0.1

d13C_THAA

mole percent weighted average ratio of stable carbon 13/12 isotopes of all amino acids. d13C_THAA = Sum(d13C_AA x molar percent AA/100). (Source: producer defined)

Range of values
Minimum:	-27.9
Maximum:	-16.3
Units:	per mil
Resolution:	0.1

d13C_THAA_SD

propagated error of mole percent weighted average ratio of stable carbon 13/12 isotopes of all amino acids. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.5
Units:	per mil
Resolution:	0.1

d15N_THAA

mole percent weighted average ratio of stable carbon 15/14 isotopes of all amino acids. d15N_THAA = Sum(d15N_AA x molar percent AA). (Source: producer defined)

Range of values
Minimum:	1.7
Maximum:	15.0
Units:	per mil
Resolution:	0.1

d15N_THAA_SD

propagated error of mole percent weighted average ratio of stable nitrogen 15/14 isotopes of all amino acids. (Source: producer defined)

Range of values
Minimum:	0.1
Maximum:	0.4
Units:	per mil
Resolution:	0.1

OC-AA_percent

percentage of total sedimentary organic carbon which is amino acids. (Source: producer defined)

Range of values
Minimum:	1.3
Maximum:	8.1
Units:	percent
Resolution:	0.1

N-AA_percent

percentage of total sedimentary nitrogen which is amino acids. (Source: producer defined)

Range of values
Minimum:	4.1
Maximum:	20.4
Units:	percent
Resolution:	0.1

Entity_and_Attribute_Overview: The first line of the csv file is a header line.
Entity_and_Attribute_Detail_Citation: U.S. Geological Survey

Who produced the data set?

Who are the originators of the data set? (may include formal authors, digital compilers, and editors)
- Hope L. Ianiri
- Nancy G. Prouty
Who also contributed to the data set?
Stephanie Christensen of the University of California, Santa Cruz, assisted with isotope ratio mass spectrometry.
To whom should users address questions about the data?

U.S. Geological Survey, Pacific Coastal and Marine Science Center

Attn: PCMSC Science Data Coordinator

2885 Mission Street
Santa Cruz, CA

831-427-4747 (voice)

pcmsc_data@usgs.gov

Why was the data set created?

Data were obtained to assess organic carbon storage, reactivity, and cycling within sediments of the Escanaba Trough, a hydrothermal spreading center which contains critical minerals. This work aids our understanding of the geochemistry of marine mineral host sediments and hydrothermal vent processes throughout the global ocean.

How was the data set created?

From what previous works were the data drawn?
How were the data generated, processed, and modified?

Date: 11-Jun-2022 (process 1 of 2)
SEDIMENT COLLECTION. Samples were collected aboard the R/V Thomas Thompson via push coring using the ROV Jason. Once recovered shipboard, pore waters were extracted via rhizon samplers, cores were subsectioned into one to five cm slices, the exterior sediment which was in contact with the core liner was removed, and remaining sediment was stored frozen in pre-combusted glass jars.
Date: 11-Apr-2024 (process 2 of 2)
COMPOUND SPECIFIC ISOTOPE ANALYSIS OF AMINO ACIDS: Amino acids were purified prior to compound specific isotope analysis of amino acids (CSI-AA) according to protocols of the McCarthy lab at the University of California, Santa Cruz (McCarthy and others, 2004). Sediments were subject to acid hydrolysis by adding 6N HCl to each sample, evacuating the headspace with N2 gas, and heating at 110 degrees Celsius for 20 hours. Samples were then mixed, centrifuged at 2200 revolutions per minute for five minutes (min), and supernatant pipetted off to new vial. This process (mixing, centrifuging, and removal of supernatant) was repeated with 1 mL 6N HCl and four 1 milliliter rinses of MilliQ H2O. The supernatant from all HCl and MilliQ H2O rinses were combined and dried under N2 gas at 80 degrees Celsius. Samples were redissolved in 0.1N HCl and a known quantity of Norleucine internal standard was added to each sample. Samples were filtered with pre-combusted glass wool followed by 1.0 micrometer glass fiber filters to remove large particles and then AA were purified using cation-exchange chromatography with Bio-Rad AG50WX8 resin (200-400 mesh) according to Takano and others (2010). Samples were dried under N2 gas at 80 degrees Celsius, redissolved in 0.2N HCl, flushed with N2 gas, and then heated at 110 degrees Celsius for 5 min to re-protonate the amino acids. Trifluoroacetyl isopropyl ester derivatives were prepared after Silfer and others (1991). A final purification step was completed via liquid-liquid extraction using chloroform and a phosphate buffer following Ueda and others (1989). Samples were dried under N2 gas at room temperature, rederivatized, and then stored at 20 degrees Celsius until analysis. Derivatized samples were analyzed for d13C-amino acid and d15N-amino acid isotope values by a Thermo Trace 1310 gas chromatograph coupled to an IsolinkII (Thermo combustion reactor 1000 degrees Celsius)/ConfloIV and Thermo Delta V Plus isotope ratio mass spectrometer. Samples were dried under N2 gas, redissolved in ethyl acetate, and injected in triplicate unless otherwise noted. Amino acids were separated for d15N analyses using a BPX5 column (60 meter x 0.32 millimeter, 1 micrometer film thickness; SGE Analytical Science, Trajan, Austin, TX, USA) and for d13C analyses using a DB-5 column (50 meter x 0.32 millimeter, 0.52 micrometer film thickness; Agilent Technologies, Santa Clara, CA, USA). The injector temperature was 250 degrees Celsius with He column flow rate of 2 mL/min. The GC temperature program for nitrogen isotope analysis was: initial temp = 70 degrees Celsius hold for 1 min; ramp 1=10 degrees Celsius/min to 185 degrees Celsius, hold for 2 min; ramp 2 = 2 degrees Celsius/min to 200 degrees Celsius, hold for 10 min; ramp 3 = 30 degrees Celsius/min to 300 degrees Celsius, hold for 6 min. The GC temperature program for carbon isotope analysis was: initial temp = 75 degrees Celsius hold for 2 min; ramp 1 = 4 degrees Celsius/min to 90 degrees Celsius, hold for 4 min; ramp 2 = 4 degrees Celsius/min to 185 degrees Celsius, hold for 5 min; ramp 3 = 10 degrees Celsius/min to 250 degrees Celsius, hold for 2 min; ramp 4 = 20 degrees Celsius/min to 300 degrees Celsius, hold for 5 min.
What similar or related data should the user be aware of?
Ianiri, Hope L., Campbell, Pamela L., Gartman, Amy, and Prouty, Nancy G., 2025, Characterizing sedimentary organic carbon in a hydrothermal spreading center, the Escanaba Trough.
Online Links:
- https://doi.org/10.1016/j.chemgeo.2025.122679
Other_Citation_Details:
Ianiri, H.L., Campbell, P., Gartman, A., and Prouty, N.G., 2025, Characterizing sedimentary organic carbon in a hydrothermal spreading center, the Escanaba Trough: Chemical Geology, https://doi.org/10.1016/j.chemgeo.2025.122679.
Morton, J. L., Zierenberg, Robert A., and Reiss, Carol A., 1994, Geologic, hydrothermal, and biologic studies at Escanaba Trough, Gorda Ridge, offshore northern California.
Online Links:
Other_Citation_Details:
Morton, J.L., Zierenberg, R.A., Reiss, C.A., 1994. Geologic, hydrothermal, and biologic studies at Escanaba Trough: an introduction, in: Geologic, Hydrothermal, and Biologic Studies at Escanaba Trough, Gorda Ridge, Offshore Northern California. U.S. Geological Survey.
Clague, David. A., Zierenberg, Robert A., Paduan, Jennifer B., Caress, David W., Cousens, Brian L., Dreyer, Brian M., Davis, Alice S., McClain, James, and Ross, Stephanie L., 2022, Emplacement and impacts of lava flows and intrusions on the sediment-buried Escanaba Segment of the Gorda mid-ocean ridge.
Online Links:
- https://www.sciencedirect.com/science/article/pii/S0377027322002323
Other_Citation_Details:
Clague, D.A., Zierenberg, R.A., Paduan, J.B., Caress, D.W., Cousens, B.L., Dreyer, B.M., Davis, A.S., McClain, J., Ross, S.L., 2022. Emplacement and impacts of lava flows and intrusions on the sediment-buried Escanaba Segment of the Gorda mid-ocean ridge. Journal of Volcanology and Geothermal Research 432, 107701.
McCarthy, Matthew D., Benner, Ronald, Lee, Cindy, Hedges, John I., and Fogel, Marilyn L., 2004, Amino acid carbon isotopic fractionation patterns in oceanic dissolved organic matter: an unaltered photoautotrophic source for dissolved organic nitrogen in the ocean?.
Online Links:
- https://doi.org/10.1016/j.marchem.2004.06.021
Other_Citation_Details:
McCarthy, M.D., Benner, R., Lee, C., Hedges, J.I., Fogel, M.L., 2004. Amino acid carbon isotopic fractionation patterns in oceanic dissolved organic matter: an unaltered photoautotrophic source for dissolved organic nitrogen in the ocean? Mar. Chem. 92, 123-134.
McCarthy, Matthew D., Lehman, Jennifer, and Kudela, Raphael, 2013, Compound-specific amino acid d15N patterns in marine algae: Tracer potential for cyanobacterial vs. eukaryotic organic nitrogen sources in the ocean..
Online Links:
- https://doi.org/10.1016/j.gca.2012.10.037
Other_Citation_Details:
McCarthy, M.D., Lehman, J., Kudela, R., 2013. Compound-specific amino acid d 15N patterns in marine algae: Tracer potential for cyanobacterial vs. eukaryotic organic nitrogen sources in the ocean. Geochim. Cosmochim. Acta 103, 104-120.
Silfer, J. A., Engel, M. H., Macko, S. A., and Jumeau, E. J., 1991, Stable carbon isotope analysis of amino acid enantiomers by conventional isotope ratio mass spectrometry and combined gas chromatography/isotope ratio mass spectrometry.
Online Links:
- https://doi.org/10.1021/ac00004a014
Other_Citation_Details:
Silfer, J.A., Engel, M.H., Macko, S.A., Jumeau, E.J., 1991. Stable carbon isotope analysis of amino acid enantiomers by conventional isotope ratio mass spectrometry and combined gas chromatography/isotope ratio mass spectrometry. Anal. Chem. 63, 370-374.
Takano, Yoshinori, Kashiyama, Yuichiro, Ogawa, Nanako O., Chikaraishi, Yoshito, and Ohkouchi, Naohiko, 2010, Isolation and desalting with cation-exchange chromatography for compound-specific nitrogen isotope analysis of amino acids: application to biogeochemical samples.
Online Links:
- https://doi.org/10.1002/rcm.4651
Other_Citation_Details:
Takano, Y., Kashiyama, Y., Ogawa, N.O., Chikaraishi, Y., Ohkouchi, N., 2010. Isolation and desalting with cation-exchange chromatography for compound-specific nitrogen isotope analysis of amino acids: application to biogeochemical samples. Rapid Commun. Mass Spectrom. 24, 2317-2323.
Ueda, Kimio, Morgan, Stephen L., Fox, Alvin, Gilbart, James, Sonesson, Anders, Larsson, Lennart, and Odham, Goran, 1989, D-Alanine as a chemical marker for the determination of streptococcal cell wall levels in mammalian tissues by gas chromatography/negative ion chemical ionization mass spectrometry..
Online Links:
- https://doi.org/10.1021/ac00178a015
Other_Citation_Details:
Ueda, Kimio., Morgan, S.L., Fox, Alvin., Gilbart, James., Sonesson, Anders., Larsson, Lennart., Odham, Goran., 1989. D-Alanine as a chemical marker for the determination of streptococcal cell wall levels in mammalian tissues by gas chromatography/negative ion chemical ionization mass spectrometry. Anal. Chem. 61, 265-270.

How reliable are the data; what problems remain in the data set?

How well have the observations been checked?
Instrument performance was monitored using both external and internal amino acid standards and directly measured d15N-amino acid and d13C-amino acid values were corrected based on bracketing external standards (McCarthy and others, 2004). The amino acid d13C values were determined from the measured values of the amino acid derivatives following the approach of Silfer and others (1991) with corrections based on an amino acid mixture standard for which isotopic values had been independently determined by offline elemental analyzer analysis. The directly measured amino acid d15N values were also corrected based on bracketing external standards as described in McCarthy and others, 2013. -8888 indicates peak was below the detection limit of 300 mV for carbon and 60 mV for nitrogen. The standard deviation of each isotope and molar percentage value was calculated from triplicate injections.
How accurate are the geographic locations?
The horizontal accuracy for each sample was obtained from the ROV's Ultra Short Baseline Positioning System (USBL) navigation after calibration to the CASIUS (Calibration of Attitude Sensors In USBL Systems) calibration system. Accuracy for this depth is approximately 10 to 20 m.
How accurate are the heights or depths?
The vertical accuracy was determined using multiple DVLs (Doppler Velocity Loggers) on the ROV, which are accurate to less than 1 m.
Where are the gaps in the data? What is missing?
Dataset is considered complete for the information presented as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
How consistent are the relationships among the observations, including topology?
Molar percentage data was checked to ensure the sum of the percentage of all individual amino acids was equivalent to one hundred percent.

How can someone get a copy of the data set?

Are there legal restrictions on access or use of the data?

Access_Constraints	No access constraints
Use_Constraints	USGS-authored or produced data and information are in the public domain from the U.S. Government and are freely redistributable with proper metadata and source attribution. Please recognize and acknowledge the U.S. Geological Survey as the originator(s) of the dataset and in products derived from these data.

Who distributes the data set? (Distributor 1 of 1)

U.S. Geological Survey - ScienceBase

Denver Federal Center, Building 810, Mail Stop 302
Denver, CO

1-888-275-8747 (voice)

sciencebase@usgs.gov
What's the catalog number I need to order this data set? These data are available in a single csv file (EscanabaTrough_organicgeochemistry_CSIAA.csv).
What legal disclaimers am I supposed to read?
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty.

How can I download or order the data?

Availability in digital form:

Data format:	csv file in format comma-delimited text (version Microsoft Excel Version 2308 Build 16731.20716 Click-to-Run) Size: 0.0714
Network links:	https://www.sciencebase.gov/catalog/file/get/6696d767d34ecb78f609f700 https://www.sciencebase.gov/catalog/item/6696d767d34ecb78f609f700 https://doi.org/10.5066/P13XVXQC

Cost to order the data: None.

What hardware or software do I need in order to use the data set?
These data can be viewed with Microsoft Excel or any text reading software.

Who wrote the metadata?

Dates:: Last modified: 18-Feb-2025
Metadata author:: U.S. Geological Survey, Pacific Coastal and Marine Science Center

Attn: PCMSC Science Data Coordinator

2885 Mission Street
Santa Cruz, CA

831-427-4747 (voice)

pcmsc_data@usgs.gov
Metadata standard:: Content Standard for Digital Geospatial Metadata (FGDC-STD-001-1998)

This page is <https://cmgds.marine.usgs.gov/catalog/pcmsc/DataReleases/ScienceBase/DR_P13XVXQC/EscanabaTrough_CSIAA.faq.html>