Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: Uptake and Inactivation Data

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What does this data set describe?
Who produced the data set?
Why was the data set created?
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What does this data set describe?

Title:

Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: Uptake and Inactivation Data

Abstract:

This metadata record describes the microbial uptake of nutrients by native planktonic and biofilm-associated bacteria and the inactivation of E. coli, MS2 bacteriophage, polio virus and Cryptosporidium parvum in the Upper Floridan Aquifer (approximately 1,000 feet below land surface) in the Okeechobee, Florida area. Groundwater samples were collected or accessed via the use of a mesocosm between 2018 and 2020 from a South Florida Water Management District (SFWMD) monitoring well installed at the Kissimmee River Aquifer Storage and Recovery (ASR) facility, which is located at the discharge point of the Kissimmee River into Lake Okeechobee.

How might this data set be cited?
Lisle, John T., 20210226, Microbial Ecology of the Floridan Aquifer near Okeechobee, FL: Uptake and Inactivation Data: U.S. Geological Survey Data Release doi:10.5066/P9EOM5RC, U.S. Geological Survey, St. Petersburg, FL.
Online Links:
- https://doi.org/10.5066/P9EOM5RC
What geographic area does the data set cover?
West_Bounding_Coordinate: -80.9280
East_Bounding_Coordinate: -80.8072
North_Bounding_Coordinate: 27.2009
South_Bounding_Coordinate: 27.0885
What does it look like?
Does the data set describe conditions during a particular time period?

Beginning_Date: 01-Jan-2018

Ending_Date: 06-Nov-2020

Currentness_Reference:

ground condition
What is the general form of this data set?
Geospatial_Data_Presentation_Form: Tabular digital data
How does the data set represent geographic features?
1. How are geographic features stored in the data set?
2. What coordinate system is used to represent geographic features?

How does the data set describe geographic features?

Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.csv, Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.xlsx

These files include data associated with the analysis of microbial nutrient uptake and physiological inactivation observed in samples collected from the Upper Floridan Aquifer. (Source: USGS)

Geochemical parameter

Groundwater geochemical parameters measured at the well head. (Source: USGS) Temperature, pH, Oxidation Reduction Potential, Specific conductance, Turbidity, Color, Total dissolved solids, Total Alkalinity (as CaCO3), Aluminum, Barium, Boron, Bromide, Calcium, Chloride, Copper, Fluoride, Iron, Magnesium, Manganese, Potassium, Silica, Sodium, Sulfate, Sulfide, Zinc, NO2-N, NO3-N, NH3-N, Total PO4-P, Ortho PO4-P, Total organic carbon, and Dissolved organic carbon.

Geochemical parameter units

Geochemical parameter units (Source: USGS)

Value	Definition
degrees Celsius	Temperature units
not applicable	There are no units for pH
millivolts	Oxidation reduction potential units
microsiemens/cm	Specific conductance units
nephelometric turbidity units (NTU)	Turbidity units
platinum cobalt units (PCU)	Color units
mg/L	Concentration units as milligrams per liter

Geochemical parameter mean and standard deviation

Arithmetic mean with 1 standard deviation value for each of the geochemical parameters. (Source: USGS) The actual measured values for each of the respective geochemical parameters.

Time (hrs) Nitrogen Planktonic

Experimental sample collection times, in hours, for the nitrogen removal experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	169.75

NO3-N (mg/L) Planktonic

The concentrations of nitrate-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.0414
Maximum:	0.4231

NO2-N (mg/L) Planktonic

The concentrations of nitrite-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.0003
Maximum:	0.3678

NH4-N (mg/L) Planktonic

The concentrations of ammonia-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.4267
Maximum:	0.4342

Time (hrs) Nitrogen Biofilm

Experimental sample collection times, in hours, for the nitrogen removal experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	169.25

NO3-N (mg/L) Biofilm

The concentrations of nitrate-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.0042
Maximum:	0.3938

NO2-N (mg/L) Biofilm

The concentrations of nitrite-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.0006
Maximum:	0.1241

NH4-N (mg/L) Biofilm

The concentrations of ammonia-nitrogen observed in samples. (Source: USGS)

Range of values
Minimum:	0.4186
Maximum:	0.4489

Time (hrs) Phosphorus Planktonic

Experimental sample collection times, in hours, for the phosphorus uptake experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	6.0

PO4-P (mg/L) Planktonic

The concentrations of phosphorus observed in samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	0.0032

Time (hrs) Phosphorus Biofilm

Experimental sample collection times, in hours, for the phosphorus uptake experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	6.0

PO4-P (mg/L) Biofilm

The concentrations of phosphorus observed in samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	0.477

Time (hrs) Carbon Planktonic

Experimental sample collection times, in hours, for the carbon uptake experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	123.0

CO2 (mg/L) Planktonic

The concentrations of carbon dioxide observed in samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	0.24479

Biomass (mg/L) Planktonic

The concentrations of carbon observed in the bacterial biomass samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	0.40887

Time (hrs) Carbon Biofilm

Experimental sample collection times, in hours, for the carbon uptake experiment. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	122.0

CO2 (mg/L) Biofilm

The concentrations of carbon dioxide observed in samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	0.38774

Biomass (mg/L) Biofilm

The concentrations of carbon observed in the bacterial biomass samples. (Source: USGS)

Range of values
Minimum:	0.0
Maximum:	1.9413

Time (hrs) MS2_1

Experimental sample collection times, in hours, for the MS2 bacteriophage in the first inactivation experiment. (Source: USGS)

Range of values
Minimum:	0.00
Maximum:	504.00

MS2_1 (PFU/mL)

The number of plaque forming units (PFU) of MS2 in inactivation samples. (Source: USGS)

Range of values
Minimum:	1
Maximum:	2085000001

Time (hrs) MS2_2

Experimental sample collection times, in hours, for the MS2 bacteriophage in the second inactivation experiment. (Source: USGS)

Range of values
Minimum:	0.00
Maximum:	886.17

MS2_2 Mean (PFU/mL)

The mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment. (Source: USGS)

Range of values
Minimum:	0
Maximum:	1585000

MS2_2 Standard Deviation (PFU/mL)

The standard deviation on the mean number of plaque forming units (PFU) of MS2 in inactivation samples in the second inactivation experiment. (Source: USGS)

Range of values
Minimum:	0
Maximum:	137961

Time (hrs) Polio

Experimental sample collection times, in hours, for the polio enterovirus inactivation experiment. (Source: USGS)

Range of values
Minimum:	0.00
Maximum:	1390.17

Polio (MPN/mL)

The median most probable number (MPN) of polio virus in each sample. (Source: USGS)

Range of values
Minimum:	35
Maximum:	3500000

Polio MPN Upper 95% Confidence Interval

The upper 95% confidence interval for the median MPN of polio virus. (Source: USGS)

Range of values
Minimum:	100
Maximum:	10000000

Polio MPN Lower 95% Confidence Interval

The lower 95% confidence interval for the median MPN of polio virus (Source: USGS)

Range of values
Minimum:	12
Maximum:	1200000

Time (hrs) Cryptosporidium parvum

Experimental sample collection times, in hours, for the Cryptosporidium parvum inactivation experiment. (Source: USGS)

Range of values
Minimum:	0.00
Maximum:	1390.17

Cryptosporidium parvum (MPN/mL)

The median most probable number (MPN) of Cryptosporidium parvum in each sample. (Source: USGS)

Range of values
Minimum:	1.00
Maximum:	28000

Cryptosporidium parvum MPN Upper 95% Confidence Interval

The upper 95% confidence interval for the median MPN of Cryptosporidium parvum. (Source: USGS)

Range of values
Minimum:	7.20
Maximum:	110000

Cryptosporidium parvum MPN Lower 95% Confidence Interval

The lower 95% confidence interval for the median MPN of Cryptosporidium parvum. (Source: USGS)

Range of values
Minimum:	0.14
Maximum:	7300

Who produced the data set?

Who are the originators of the data set? (may include formal authors, digital compilers, and editors)
- John T. Lisle
Who also contributed to the data set?
USGS and South Florida Water Management District.
To whom should users address questions about the data?

John T Lisle

Southeast Region: ST. PETE COASTAL & MARINE SC

Research Microbiologist

600 4Th Street South
St. Petersburg, FL

United States

727-502-8140 (voice)

jlisle@usgs.gov

Why was the data set created?

Data were collected to quantify the nutrient uptake and microbial inactivation rates under native groundwater geochemical conditions.

How was the data set created?

From what previous works were the data drawn?
How were the data generated, processed, and modified?

Date: 01-Aug-2019 (process 1 of 2)
Between 2018 and 2020 groundwater samples were collected from a SFWMD monitoring well located at a study site near Lake Okeechobee, Florida to conduct two types of experiments. The first was a nutrient uptake experiment where a standardized solution of nitrogen (KNO3), phosphorus (K2PO4) and carbon (Na-acetate) was added to each of the respective experimental solutions, then sampled over a period of time to quantify the rates at which the native planktonic and biofilm-associated bacteria removed these nutrients and either incorporated them into bacterial biomass and/or carbon dioxide. The data from the nitrogen removal experiments were generated on a Seal Analytical Auto Analyzer 3, while the data from the phosphorus and carbon uptake experiments were generated on a Perkin Elmer TriCarb liquid scintillation counter following the measurement of samples containing 32P (as phosphate) and 14C (as acetate).
Date: 06-Nov-2020 (process 2 of 2)
Between January and October 2020 known concentrations of E. coli, bacteriophage MS2, human enterovirus polio, and Cryptosporidium parvum were added to filtered sterilized groundwater from the same SFWMD monitoring well used for the nutrient uptake experiments and described in the previous step. These solutions of microorganisms were then transferred to diffusion chambers that were suspended in a constant flow of groundwater within a mesocosm at the wellhead. Periodically, a diffusion chamber for each of the microorganisms was recovered from the mesocosm and processed for the recovery of each type of microorganism using methods specific to the recovery of that microorganism.
What similar or related data should the user be aware of?

How reliable are the data; what problems remain in the data set?

How well have the observations been checked?
No formal attribute accuracy tests were conducted.
How accurate are the geographic locations?
No formal positional accuracy tests were conducted.
How accurate are the heights or depths?
No formal positional accuracy tests were conducted.
Where are the gaps in the data? What is missing?
Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
How consistent are the relationships among the observations, including topology?
No formal logical accuracy tests were conducted.

How can someone get a copy of the data set?

Are there legal restrictions on access or use of the data?

Access_Constraints	None
Use_Constraints	Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. The U.S. Geological Survey (USGS) requests to be acknowledged as originator of these data in future products or derivative research.

Who distributes the data set? (Distributor 1 of 1)

John T Lisle

Southeast Region: ST. PETE COASTAL & MARINE SC

Research Microbiologist

600 4Th Street South
St. Petersburg, FL

United States

727-502-8140 (voice)

jlisle@usgs.gov
What's the catalog number I need to order this data set?
What legal disclaimers am I supposed to read?
Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

How can I download or order the data?

Availability in digital form:

Data format:	CSV, XLXS
Network links:	https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_Data.zip https://coastal.er.usgs.gov/data-release/doi-P9EOM5RC/data/Upper_Floridan_Aquifer_Microbial_Nutrient_Uptake_And_Inactivation_Data.zip

Cost to order the data: None

Who wrote the metadata?

Dates:: Last modified: 07-Dec-2020
Metadata author:: John T Lisle

Southeast Region: ST. PETE COASTAL & MARINE SC

Research Microbiologist

600 4Th Street South
St. Petersburg, FL

United States

727-502-8140 (voice)

jlisle@usgs.gov
Metadata standard:: Content Standard for Digital Geospatial Metadata (FGDC-STD-001-1998)

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